MOESM7 of Translation efficiency of heterologous proteins is significantly affected by the genetic context of RBS sequences in engineered cyanobacterium Synechocystis sp. PCC 6803

Additional file 7. Expression levels predicted by UTR Designer for sYFP2 and GFPmut3b in combination with the 13 target RBS sequences used in the study

MOESM1 of Translation efficiency of heterologous proteins is significantly affected by the genetic context of RBS sequences in engineered cyanobacterium Synechocystis sp. PCC 6803

Additional file 1. Nucleotide sequence of the synthetic GFPmut3b gene codon optimized for Synechosystis sp. PCC 6803

MOESM4 of Translation efficiency of heterologous proteins is significantly affected by the genetic context of RBS sequences in engineered cyanobacterium Synechocystis sp. PCC 6803

Additional file 4. The full fluorescence activity profiles of the generated Synechocystis sp. PCC 6803 strains expressing sYFP2 and GFPmut3b under the control of the 13 selected RBSs (0–6 h)

MOESM3 of Translation efficiency of heterologous proteins is significantly affected by the genetic context of RBS sequences in engineered cyanobacterium Synechocystis sp. PCC 6803

Additional file 3. Colony PCR verification of the Synechocystis sp. PCC 6803 strains generated in this study as visualized by agarose gel electrophoresis