Интенсивность хемилюминесценции, состояние антиоксидантной системы и окислительная модификация белков плазмы крови при развитии рецидива рака яичников

The state of antioxidant system (the activity of catalase and ceruloplasmine), the intensity of chemiluminescence, and oxidative modification of proteins were studied in blood plasma of 61 patients with ovarian cancer (cystadenocarcinoma) III–IV stage in state of remission and ones with a local recurrent tumor. The dynamics of these indices was analyzed in dependence of recurrent tumor vascularization. The statistically significant changes of the activity of some links of antioxidant system were found, as well as chemiluminescence intensity increase in blood plasma. The level of oxidative modificated protein molecules was raised, the most expressed for products of the main character (530 nm). The dynamics of level of carbonyl derivatives of the neutral and main character (370 and 530 nm) was opposite directed during intensification of recurrent tumour vascularization and increase in speed of blood-groove in a recurrent tumorУ 61 больной раком яичников (цистаденокарцинома) III–IV стадий в состоянии ремиссии и с локальным рецидивом ис- следованы в плазме крови интенсивность хемилюминесценции, состояние ряда звеньев антиоксидантной системы (активность каталазы, церулоплазмина) и уровень окислительной модификации белков общепринятыми спектрофотометрическими методами. Проанализирована динамика изученных показателей в крови больных с рецидивом в зависимости от интенсивности кровотока в рецидивной опухоли. Обнаружено достоверное изменение активности некоторых звеньев антиоксидантной системы, усиление хемилюминесценции плазмы крови. Показано увеличение степени окисленности белковых молекул, наиболее выраженное для продуктов основного характера (530 нм). По мере прогрессирования онкологического процесса при формировании рецидивной опухоли, усилении ее васкуляризации и увеличении скорости кровотока наблюдается противоположно направленная динамика уровня карбонильных производных нейтрального и основного характера (370 и 530 нм

CHEMILUMINESCENCE INTENSITY, ANTIOXIDANT SYSTEM STATE AND OXIDATIVE MODIFICATION OF PROTEINS IN BLOOD IN OVARIAN CANCER PATIENTS WITH A LOCAL RECURRENT TUMOR

The state of antioxidant system (the activity of catalase and ceruloplasmine), the intensity of chemiluminescence, and oxidative modification of proteins were studied in blood plasma of 61 patients with ovarian cancer (cystadenocarcinoma) III–IV stage in state of remission and ones with a local recurrent tumor. The dynamics of these indices was analyzed in dependence of recurrent tumor vascularization. The statistically significant changes of the activity of some links of antioxidant system were found, as well as chemiluminescence intensity increase in blood plasma. The level of oxidative modificated protein molecules was raised, the most expressed for products of the main character (530 nm). The dynamics of level of carbonyl derivatives of the neutral and main character (370 and 530 nm) was opposite directed during intensification of recurrent tumour vascularization and increase in speed of blood-groove in a recurrent tumo

Activation of different growth factor systems in tissues of adenocarcinoma and signet-ring cell carcinoma of the stomach

Purpose of the study. The purpose of the study was to find the difference between local levels of VEGF, EGF, IGFI and IG- FII in tumor and intact tissues in adenocarcinoma of the stomach (AC) and signet-ring cell carcinoma of the stomach (SRCC).Materials and methods. Levels of VEGF, EGF, EGFR, IGF-I and IGFII were determined by ELISA in tumor and intact gastric tissues of 15 AC patients and 10 SRCC patients (T2-3N0-2M0, G2-3), mean age 62.8±2.38 years.Results. Levels of IGF-I, IGF-II and VEGF in intact gastric tissues were similar at AC and SRCC, but EGF and EGFR levels at AC were higher than at SRCC. Level of VEGF in AC tumor tissue, compared to intact gastric tissue, increased by 2.3 times and IGFI and IGFII decreased by 2.1 and 1.8 times, respectively. Tumor tissue of SRCC showed the increased level of EGF (by 2.9 times) and EGFR (by 1.8 times), tissue of SRCC was characterized by the activation of system of EGF and IGF, but not VEGF. Tissue of AC demonstrated the stimulation of VEGFA neoangiogenesis.Conclusion. A significant difference was observed in the levels of VEGF-A, IGF-I and IGF-II and components of the EGF system in gastric tumors and visually intact tissues in G2-G3 AC and SRCC. The higher activity of the EGF and IGF systems in SRCC is probably associated with diffuse growth characteristics and the origin from neuroendocrine cells, which serves as an autocrine/paracrine regulation of invasive growth. AC with solid structure is more in need of neoangiogenesis carried out mainly by the VEGF system