88 research outputs found
Covalent modification of engineered cysteines in the nicotinic acetylcholine receptor agonist-binding domain inhibits receptor activation
Clinical characteristics of Polish patients with ANCA-associated vasculitides-retrospective analysis of POLVAS registry
Objective Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV) are rare small to medium-size
vessel systemic diseases. As their clinical picture, organ involvement, and factors influencing outcome may differ
between countries and geographical areas, we decided to describe a large cohort of Polish AAV patients coming from
several referral centers-members of the Scientific Consortium of the Polish Vasculitis Registry (POLVAS).
Methods We conducted a systematic multicenter retrospective study of adult patients diagnosed with AAV between
Jan 1990 and Dec 2016 to analyze their clinical picture, organ involvement, and factors influencing outcome. Patients
were enrolled to the study by nine centers (14 clinical wards) from seven Voivodeships populated by 22.3 mln inhabitants (58.2% of the Polish population).
Results Participating centers included 625 AAV patients into the registry. Their distribution was as follows: 417 patients
(66.7%) with GPA, 106 (17.0%) with MPA, and 102 (16.3%) with EGPA. Male-to-female ratios were almost 1:1 for
GPA (210/207) and MPA (54/52), but EGPA was twice more frequent among women (34/68). Clinical manifestations
and organ involvement were analyzed by clinical phenotype. Their clinical manifestations seem very similar to other
European countries, but interestingly, men with GPA appeared to follow a more severe course than the women. Fifty five
patients died. In GPA, two variables were significantly associated with death: permanent renal replacement therapy
(PRRT) and respiratory involvement (univariate analysis). In multivariate analysis, PRRT (OR = 5.3; 95% confidence interval (CI) = 2.3–12.2), respiratory involvement (OR = 3.2; 95% CI = 1.06–9.7), and, in addition, age > 65 (OR = 2.6;
95% CI = 1.05–6.6) were independently associated with death. In MPA, also three variables were observed to be
independent predictors of death: PRRT (OR = 5.7; 95% CI = 1.3–25.5), skin involvement (OR = 4.4; 95% CI = 1.02–
19.6), and age > 65 (OR = 6.3; 95% CI = 1.18–33.7).
Conclusions In this first multicenter retrospective study of the Polish AAV patients, we have shown that their demographic
characteristics, disease manifestations, and predictors of fatal outcome follow the same pattern as those from other European
countries, with men possibly suffering from more severe course of the disease
Binding of alpha-bungarotoxin to isolated alpha subunit of the acetylcholine receptor of Torpedo californica: quantitative analysis with protein blots.
Mutations affecting agonist sensitivity of the nicotinic acetylcholine receptor
The nicotinic acetylcholine receptor (AChR) is a pentameric transmembrane protein (alpha 2 beta gamma delta) that binds the neurotransmitter acetylcholine (ACh) and transduces this binding into the opening of a cation selective channel. The agonist, competitive antagonist, and snake toxin binding functions of the AChR are associated with the alpha subunit (Kao et al., 1984; Tzartos and Changeux, 1984; Wilson et al., 1985; Kao and Karlin, 1986; Pederson et al., 1986). We used site-directed mutagenesis and expression of AChR in Xenopus oocytes to identify amino acid residues critical for ligand binding and channel activation. Several mutations in the alpha subunit sequence were constructed based on information from sequence homology and from previous biochemical (Barkas et al., 1987; Dennis et al., 1988; Middleton and Cohen, 1990) and spectroscopic (Pearce and Hawrot, 1990; Pearce et al., 1990) studies. We have identified one mutation, Tyr190 to Phe (Y190F), that had a dramatic effect on ligand binding and channel activation. These mutant channels required more than 50-fold higher concentrations of ACh for channel activation than did wild type channels. This functional change is largely accounted for by a comparable shift in the agonist binding affinity, as assessed by the ability of ACh to compete with alpha-bungarotoxin binding. Other mutations at nearby conserved positions of the alpha subunit (H186F, P194S, Y198F) produce less dramatic changes in channel properties. Our results demonstrate that ligand binding and channel gating are separable properties of the receptor protein, and that Tyr190 appears to play a specific role in the receptor site for acetylcholine
Determination of the primary amino acid sequence specifying the alpha-bungarotoxin binding site on the alpha subunit of the acetylcholine receptor from Torpedo californica.
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