Assessing the social cohesion of a translocated pride of white lions integrated with wild tawny lions in South Africa, using social network analysis

In South Africa, lions are protected in national parks and smaller fenced reserves. Translocating lions between fenced reserves, whilst necessary to maintain genetic diversity, is disruptive and can impact survivorship and pride cohesion. Critical to translocation success is pride cohesion. White lions are a natural colour variant occurring in the Greater Kruger Park Region, where anthropogenic threats eliminated this population until reintroduction in 2006. Through social network analysis (SNA), the sociality of a released pride of captive-origin white and wild tawny lions was compared to two captive-origin and wild prides of tawny lions. Social interactions and pride dynamics were recorded for each pride. For all prides, cubs and subadults were central to the play network, while adults received the most social interactions. White and wild tawny adult males initiated more social interactions than captive-origin tawny males, whilst a keystone adult female was identified in each pride. For the constructed pride, social interactions were more evenly distributed, suggesting a high level of connectedness and cohesion. This is the first study to demonstrate that captive-origin white and wild tawny lions can form a socially functional pride, suggesting that white lions would survive in the wild in the absence of anthropogenic threats

Home range and movement patterns of reintroduced white lions (Panthera leo melanochaita) in the Kruger to Canyons Biosphere Reserve, South Africa

White lions are a colour variant of the African lion Panthera leo melanochaita and disappeared from the wild due to anthropogenic factors until their reintroduction to the Greater Kruger Park Region of South Africa in 2006. Natural home range behaviour is an index of reintroduction success. Therefore, the home range and movement of a pride of reintroduced white lions and a constructed pride consisting of reintroduced white lions and translocated wild tawny lionesses in small, fenced reserves was assessed. GPS data from collared adults were collected for the white lion pride between 2010–2011 and 2018–2020 for the constructed pride. Home ranges were estimated using kernel density estimation and minimum convex polygon, with minimum daily distance tested for differences between sex, season, and pride. Home ranges were small and average daily movements restricted for both prides (white lion pride: 5.41 km2 and 10.44 ± 4.82 km; constructed pride: 5.50 km2, 11.37 ± 4.72 km) due to the small reserve size of 7 km2. There was no difference between prides for annual and seasonal home range size, male and female home ranges, minimum daily distance travelled, or habitat selection. White lions from both prides established territories and displayed natural home ranging behaviour, suggesting that their reintroduction was successful, in the absence of anthropogenic threats

Australian livestock export industry workers’ attitudes toward animal welfare

Understanding live export industry workers’ attitudes and beliefs toward animal welfare can provide insight into their decision-making processes and likely behavior. Industry workers (n = 265) with various roles within the supply chain were surveyed from different global regions. Participants were divided into ten categories according to their industry roles and compared using ordinal regression. Respondents were highly likely to have a positive attitude toward animal welfare; the majority of workers enjoyed working with livestock (95.8%) and agreed that livestock should be treated with respect (97.7%). Workers demonstrated a strong understanding of animal welfare concepts, 168 respondents (63.4%) provided examples of ways they had improved animal welfare in their workplace, and 164 workers (61.9%) suggested ways that animal welfare could be improved further. Most workers (95.8%) agreed that animal welfare was satisfactory in their workplace. Five out of the 24 multiple-choice responses differed significantly by the participant’s industry role, but no particular group displayed consistently divergent beliefs or attitudes. Given the community concern regarding animals in the livestock export supply chain, it is imperative to understand the attitudes of industry personnel who are responsible for the daily management of the animals. This knowledge assists in the development of animal welfare policy and can inform strategies to manage public perception

Assessing the social cohesion of a translocated pride of white lions integrated with wild tawny lions in South Africa, using social network analysis

In South Africa, lions are protected in national parks and smaller fenced reserves. Translocating lions between fenced reserves, whilst necessary to maintain genetic diversity, is disruptive and can impact survivorship and pride cohesion. Critical to translocation success is pride cohesion. White lions are a natural colour variant occurring in the Greater Kruger Park Region, where anthropogenic threats eliminated this population until reintroduction in 2006. Through social network analysis (SNA), the sociality of a released pride of captive-origin white and wild tawny lions was compared to two captive-origin and wild prides of tawny lions. Social interactions and pride dynamics were recorded for each pride. For all prides, cubs and subadults were central to the play network, while adults received the most social interactions. White and wild tawny adult males initiated more social interactions than captive-origin tawny males, whilst a keystone adult female was identified in each pride. For the constructed pride, social interactions were more evenly distributed, suggesting a high level of connectedness and cohesion. This is the first study to demonstrate that captive-origin white and wild tawny lions can form a socially functional pride, suggesting that white lions would survive in the wild in the absence of anthropogenic threats.Conservation Biolog

Animal welfare indicators for sheep during sea transport: The effect of voyage day and time of day

Ensuring the well-being of animals during transport is becoming an increasingly important societal concern. The Australian livestock export industry recognises the need for comprehensive monitoring and reporting on animal welfare during sea transport. It is predicted that pen-side assessments of sheep can be used to monitor environmental conditions, resource access, and animal health and behavioural outcomes throughout a sea voyage. Pen-side assessments by observation are non-invasive and practical to apply in an industry setting. This study monitored sheep using a pilot list of welfare indicators during two sea voyages from Australia to the Middle East, in contrasting seasons. Sheep behaviour, environment and resources were recorded three times daily via pen-side observations of six pens of Merino wethers (castrated males), repeated over three decks for each voyage. Behavioural outcomes were examined for the effect of sampling frequency on group assessments. The number of behavioural measures were reduced via Principal Component (PC) analysis. The primary three PC factors were tested against the time of sampling and pen location after accounting for the effect of environmental- and resource-based predictor variables. PC 1 (24.0 % of the total variance) described activity levels, with sheep on Voyage B being more active in the morning and resting or recumbent in the middle of the afternoon and evening. PC 2 (14.7 %) reflected heat responses with the majority of the variation in these data accounted for by changes in Wet Bulb Globe Temperature (WBGT) and manure pad moisture. The heat responses described by PC 2 also varied by voyage day (p < 0.001) and time point (p < 0.001). PC 3 scores (9.5 %) reflected flight distances and feeding behaviour and strongly correlated to WBGT and pellet consumption per head per day. Feeding behaviour generally became more competitive, and flight distances reduced as both voyages progressed. Results indicate that a comprehensive welfare monitoring protocol requires repeated daily sampling throughout a voyage. The findings of this study are pertinent for developing a sampling strategy to assess sheep welfare during sea transport

Review of livestock welfare indicators relevant for the Australian live export industry

Animal welfare is an important issue for the live export industry (LEI), in terms of economic returns, community attitudes and international socio-political relations. Mortality has traditionally been the main welfare measure recorded within the LEI; however, high mortality incidents are usually acted upon after adverse events occur, reducing the scope for proactive welfare enhancement. We reviewed 71 potential animal welfare measures, identifying those measures that would be appropriate for use throughout the LEI for feeder and slaughter livestock species, and categorised these as animal-, environment- and resource-based. We divided the live export supply chain into three sectors: (1) Australian facilities, (2) vessel and (3) destination country facilities. After reviewing the relevant regulations for each sector of the industry, we identified 38 (sector 1), 35 (sector 2) and 26 (sector 3) measures already being collected under current practice. These could be used to form a ‘welfare information dashboard’: a LEI-specific online interface for collecting data that could contribute towards standardised industry reporting. We identified another 20, 25 and 28 measures that are relevant to each LEI sector (sectors 1, 2, 3, respectively), and that could be developed and integrated into a benchmarking system in the future

Home range and movement patterns of reintroduced white lions (Panthera leo melanochaita) in the Kruger to Canyons Biosphere Reserve, South Africa

White lions are a colour variant of the African lion Panthera leo melanochaita and disappeared from the wild due to anthropogenic factors until their reintroduction to the Greater Kruger Park Region of South Africa in 2006. Natural home range behaviour is an index of reintroduction success. Therefore, the home range and movement of a pride of reintroduced white lions and a constructed pride consisting of reintroduced white lions and translocated wild tawny lionesses in small, fenced reserves was assessed. GPS data from collared adults were collected for the white lion pride between 2010-2011 and 2018-2020 for the constructed pride. Home ranges were estimated using kernel density estimation and minimum convex polygon, with minimum daily distance tested for differences between sex, season, and pride. Home ranges were small and average daily movements restricted for both prides (white lion pride: 5.41 km2 and 10.44 ± 4.82 km; constructed pride: 5.50 km2, 11.37 ± 4.72 km) due to the small reserve size of 7 km2. There was no difference between prides for annual and seasonal home range size, male and female home ranges, minimum daily distance travelled, or habitat selection. White lions from both prides established territories and displayed natural home ranging behaviour, suggesting that their reintroduction was successful, in the absence of anthropogenic threats.Environmental Biolog

Animal welfare indicators for sheep during sea transport: Monitoring health and behaviour

The welfare of sheep transported by sea is a pertinent issue for Australia’s agriculture industry and a subject of concern amongst the general public. Evaluating the effect of sea transport on the welfare of livestock requires an accurate system of reporting. This study piloted an animal welfare monitoring protocol during two sea voyages from Australia to the Middle East. Sheep health and behaviour were analysed to determine if the protocol could detect animal responses to voyage conditions such as feeding management and changes to the pen environment. Data were recorded for target pens of five different commercial lines of Merino sheep. Principle Components (PC) analysis on behavioural data identified three principal components which were compared with voyage day, management and environmental conditions using general linear mixed modelling (GLMM). PC factor 1 scores (23.35 % of total variance) showed that resting and recumbency increased as the voyage progressed. PC factor 2 scores (17.09 % variance) demonstrated that behavioural responses to heat fluctuated by voyage day. PC factor 3 scores (9.48 % variance) showed reduced flight distances and increasingly competitive feeding behaviour as the voyage progressed. Environment and management conditions were associated with behavioural changes, with Wet Bulb Globe Temperature (WBGT) being the most influential measure affecting all three PC factors (p < 0.001). There were few diseases or symptoms of ill-health (including veterinary treatments) or mortalities recorded for sample pens; however, associations between ill-health and nutrition, environmental conditions and behaviour, were identified using GLMM. Increases in WBGT were linked to increased nasal discharge (p < 0.001), ocular discharge (p < 0.05), pink eye (p < 0.05) and Panting Score 4 (p < 0.01). With few recordings of ill-health, and a low mortality incidence, it was difficult to interpret the results of comparisons between health and behaviour. It is likely that associations between health indicators and behavioural variations will be more meaningful if a welfare monitoring protocol were applied to a larger sample size. The pen-side assessments presented in this study provided a practicable and comprehensive system for monitoring the behaviour and health of sheep during sea voyages. The application of such assessments on an industry basis could better inform all stakeholders about the welfare of sheep during sea transport

Impact of shearing sheep on feeding and behaviour during the pre-embarkment feedlot phase of live export

Context: Shearing is a management procedure that can induce stress, and is one that sheep often undergo on-farm and/or at registered premises during the pre-embarkation phase of live export. Aim: As inanition is one of the largest causes of death during sea transport and can result from sheep experiencing stress, whether shearing results in behavioural changes and stress before boarding a vessel needs to be determined. Methods: A total of 600 merino wethers sourced from one farm were housed at registered pre-embarkment premises, where they were split between two adjacent pens. The wethers were further split into the six treatment groups (unshorn, and sheep shorn on Days 1, 2, 3, 4 or 5) with 50 sheep per treatment, per pen. All wethers were fitted with radio frequency identification tags that were used to record animal presence at feed and water troughs, and coloured ear tags to visually identify their treatment group. Temperature and humidity sensors were placed on the outside of each pen to record environmental conditions throughout the 13-day study. Body condition scores for each wether were recorded at the beginning and end of the study. Four portable cameras were placed at a corner of each pen, allowing behaviour states (standing, lying or locomotion) of the wethers to be recorded on Days 1, 2, 3, 4, 5 and 13 of the study. Key results: Shorn treatment groups were observed to spend more time standing (F5,5 = 6.63, P = 0.029) and less time lying (F23,23 = 2.48, P = 0.017). The control group spent more time at water troughs from Day 4 to 12 (F5,55 = 3.98, P < 0.01), whereas all groups were recorded as spending more time at the feed trough on Day 5 (F5,55 = 2.78, P < 0.001). Conclusions: A lack of variation in behavioural states between the treatments and no change in time spent feeding throughout the study suggest that the procedure of shearing within the first 5 days at the pre-embarkation facility had no impact on behaviour of these sheep. Implications: These results assist the industry in ensuring best management practices are implemented