6 research outputs found
Effect of Solvent on Drug Release and a Spray-Coated Matrix of a Sirolimus-Eluting Stent Coated with Poly(lactic-<i>co</i>-glycolic acid)
Sirolimus
(SRL) release from the biodegradable polyÂ(l-lactic-<i>co</i>-glycolic acid) (PLGA) matrix was investigated for the
application of drug-eluting stents (DES). In particular, this study
focused on whether various
organic solvents affect the interaction between SRL and PLGA and the
formation of microstructures during ultrasonic coating. The SRL-loaded
PLGA coated by tetrahydrofuran or acetone showed a significant initial
burst, whereas that from acetonitrile was constantly released during
a period of 21 days. On the basis of these results, the interactions
at the molecular level of SRL with the polymer matrix were estimated
according to various organic solvents. Although the topographies of
the coated surface were obviously different, the correlation between
surface roughness and SRL release was very poor. Irrespective of organic
solvents, FT-IR data showed significantly weak SRL-PLGA interactions.
From the result of wide-angle X-ray diffraction, it was confirmed
that SRL was dispersed in an amorphous state in the polymer matrix
after ultrasonic coating. The glass-transition temperature was also
influenced by organic solvents, resulting in a plasticizing effect.
The particle size of SRL appeared to determine the release profile
from the PLGA matrix, which was the combination of diffusion and polymer
degradation at an SRL size of more than 800 nm and the Fickian release
at that of less than 300 nm. Therefore, organic solvents can lead
to a heterogeneous microstructure in the SRL-loaded PLGA matrix, which
is at or near the surface, consisting of aggregated drug- and polymer-rich
regions. It is expected that the drug release can be controlled by
physicochemical properties of organic solvents, and this study can
be used effectively for localized drug release in biomedical devices
such as drug-eluting stents
Additional file 1 of Multiple treatments with human embryonic stem cell-derived mesenchymal progenitor cells preserved the fertility and ovarian function of perimenopausal mice undergoing natural aging
Supplementary Material 1: Fig. 1. Impact of hESC-MPCs on MDSCs expansion in an in vitro system. (A) Representative FACS plot of MDSCs isolated from the spleen. (B) MDSC cell numbers after co-culture with hESC-MPCs. (*p < 0.05)
Mussel-Mimetic Protein-Based Adhesive Hydrogel
Hydrogel systems based on cross-linked
polymeric materials which
could provide both adhesion and cohesion in wet environment have been
considered as a promising formulation of tissue adhesives. Inspired
by marine mussel adhesion, many researchers have tried to exploit
the 3,4-dihydroxyphenylalanine (DOPA) molecule as a cross-linking
mediator of synthetic polymer-based hydrogels which is known to be
able to achieve cohesive hardening as well as adhesive bonding with
diverse surfaces. Beside DOPA residue, composition of other amino
acid residues and structure of mussel adhesive proteins (MAPs) have
also been considered important elements for mussel adhesion. Herein,
we represent a novel protein-based hydrogel system using DOPA-containing
recombinant MAP. Gelation can be achieved using both oxdiation-induced
DOPA quinone-mediated covalent and Fe<sup>3+</sup>-mediated coordinative
noncovalent cross-linking. Fe<sup>3+</sup>-mediated hydrogels show
deformable and self-healing viscoelastic behavior in rheological analysis,
which is also well-reflected in bulk adhesion strength measurement.
Quinone-mediated hydrogel has higher cohesive strength and can provide
sufficient gelation time for easier handling. Collectively, our newly
developed MAP hydrogel can potentially be used as tissue adhesive
and sealant for future applications
Biomimetic Porous PLGA Scaffolds Incorporating Decellularized Extracellular Matrix for Kidney Tissue Regeneration
Chronic kidney disease is now recognized
as a major health problem, but current therapies including dialysis
and renal replacement have many limitations. Consequently, biodegradable
scaffolds to help repairing injured tissue are emerging as a promising
approach in the field of kidney tissue engineering. PolyÂ(lactic-<i>co</i>-glycolic acid) (PLGA) is a useful biomedical material,
but its insufficient biocompatibility caused a reduction in cell behavior
and function. In this work, we developed the kidney-derived extracellular
matrix (ECM) incorporated PLGA scaffolds as a cell supporting material
for kidney tissue regeneration. Biomimetic PLGA scaffolds (PLGA/ECM)
with different ECM concentrations were prepared by an ice particle
leaching method, and their physicochemical and mechanical properties
were characterized through various analyses. The proliferation of
renal cortical epithelial cells on the PLGA/ECM scaffolds increased
with an increase in ECM concentrations (0.2, 1, 5, and 10%) in scaffolds.
The PLGA scaffold containing 10% of ECM has been shown to be an effective
matrix for the repair and reconstitution of glomerulus and blood vessels
in partially nephrectomized mice in vivo, compared with only PLGA
control. These results suggest that not only can the tissue-engineering
techniques be an effective alternative method for treatment of kidney
diseases, but also the ECM incorporated PLGA scaffolds could be promising
materials for biomedical applications including tissue engineered
scaffolds and biodegradable implants
sj-tif-2-tej-10.1177_20417314231226105 – Supplemental material for Therapeutic potential of luteolin-loaded poly(lactic-co-glycolic acid)/modified magnesium hydroxide microsphere in functional thermosensitive hydrogel for treating neuropathic pain
Supplemental material, sj-tif-2-tej-10.1177_20417314231226105 for Therapeutic potential of luteolin-loaded poly(lactic-co-glycolic acid)/modified magnesium hydroxide microsphere in functional thermosensitive hydrogel for treating neuropathic pain by So-Yeon Park, Joon Hyuk Jung, Da-Seul Kim, Jun-Kyu Lee, Byeong Gwan Song, Hae Eun Shin, Ji-Won Jung, Seung-Woon Baek, Seungkwon You, Inbo Han and Dong Keun Han in Journal of Tissue Engineering</p
sj-docx-1-tej-10.1177_20417314231226105 – Supplemental material for Therapeutic potential of luteolin-loaded poly(lactic-co-glycolic acid)/modified magnesium hydroxide microsphere in functional thermosensitive hydrogel for treating neuropathic pain
Supplemental material, sj-docx-1-tej-10.1177_20417314231226105 for Therapeutic potential of luteolin-loaded poly(lactic-co-glycolic acid)/modified magnesium hydroxide microsphere in functional thermosensitive hydrogel for treating neuropathic pain by So-Yeon Park, Joon Hyuk Jung, Da-Seul Kim, Jun-Kyu Lee, Byeong Gwan Song, Hae Eun Shin, Ji-Won Jung, Seung-Woon Baek, Seungkwon You, Inbo Han and Dong Keun Han in Journal of Tissue Engineering</p