Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry

In the aviculture industry, the use of Lactobacillus spp. as a probiotic has been shown to be frequent and satisfactory, both in improving bird production indexes and in protecting intestine against colonization by pathogenic bacteria. Adhesion is an important characteristic in selecting Lactobacillus probiotic strains since it impedes its immediate elimination to enable its beneficial action in the host. This study aimed to isolate, identify and characterize the in vitro and in vivo adhesion of Lactobacillus strains isolated from birds. The Lactobacillus spp. was identified by PCR and sequencing and the strains and its adhesion evaluated in vitro via BMM cell matrix and in vivo by inoculation in one-day-old birds. Duodenum, jejunum, ileum and cecum were collected one, four, 12 and 24 h after inoculation. The findings demonstrate greater adhesion of strains in the cecum and an important correlation between in vitro and in vivo results. It was concluded that BMM utilization represents an important technique for triage of Lactobacillus for subsequent in vivo evaluation, which was shown to be efficient in identifying bacterial adhesion to the enteric tract.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Eradication of Salmonella Typhimurium in broiler chicks by combined use of P22 bacteriophage and probiotic

<span style="line-height: 150%;" lang="EN-US">It has been reported that the phage therapy is effective in controlling the number of colony-forming unit (CFU) of <em style="mso-bidi-font-style: normal;">Salmonella</em> spp. in chicken gut. This paper describes the protective effect of phage and <em style="mso-bidi-font-style: normal;">Lactobacilli</em> administration on <em style="mso-bidi-font-style: normal;">Salmonella</em> infection in 1-day-old chicks. We administered the bacteriophage P22 in a single dose and a probiotic mixture of four species of bacteriocin-producing <em style="mso-bidi-font-style: normal;">Lactobacillus</em> once a day for one week. Samples were analyzed every 48 hours, and intestinal eradication of <em style="mso-bidi-font-style: normal;">S.</em> Typhimurium was confirmed after treatments. We observed an increase in the size of duodenal villi and cecal crypts, as well as an increase in body weight in groups that received daily doses of <em style="mso-bidi-font-style: normal;">Lactobacilli</em>. </span><span style="line-height: 150%;" lang="EN-US">This study confirms the efficiency of bacteriophage therapy in controlling salmonellosis in chicks and the beneficial effect of <em style="mso-bidi-font-style: normal;">Lactobacilli</em> mixtures in the weight gain of the birds.<span style="mso-spacerun: yes;"> </span></span

Bacteriophage- induced reduction in Salmonella Enteritidis counts in the crop of broiler chickens undergoing preslaughter feed withdrawal

Salmonella food poisoning is a public health problem. Feed withdrawal from broiler chickens before slaughter can favor the multiplication of Salmonella in the cecum and crop of contaminated animals and subsequently lead to contamination of carcasses in the processing plant. In the present study, a cocktail of lytic bacteriophages isolated from sewage water was orally administered to 45-d-old broiler chickens 1 h after they received an oral dose of 107 cfu/mL Salmonella enterica subspecies enterica serotype Enteritidis. Immediately after phage administration and 30 min, 1, 3, 6, and 12 h thereafter, groups of chicken were killed. Ceca and crops were analyzed for the presence of Salmonella. At 3 h posttreatment, there were 103 cfu/g and 101 cfu/g of cecal and crop suspension, respectively. At 6 h after treatment, the number of Salmonella was 103 cfu/g in the cecal suspension, but below the detection limit in the crops. our results suggest that bacteriophage therapy may be able to reduce the contamination of chicken carcasses by reducing the preslaughter load of Salmonella in the birds