Collagen P4H isoenzyme gene expression in articular chondrocytes aggregates

<p><b>Copyright information:</b></p><p>Taken from "Human meniscus cells express hypoxia inducible factor-1α and increased SOX9 in response to low oxygen tension in cell aggregate culture"</p><p>http://arthritis-research.com/content/9/4/R69</p><p>Arthritis Research & Therapy 2007;9(4):R69-R69.</p><p>Published online 18 Jul 2007</p><p>PMCID:PMC2206369.</p><p></p> Prolyl 4-hydroxylase (P4H)α isoenzyme gene expression in cell aggregates of articular chondrocytes in 20% oxygen (white bars) and 5% oxygen (black bars). Data are expressed as mean ± standard deviation (= 3)

Gene expression of HIF-1α and target genes in the outer and inner meniscus

<p><b>Copyright information:</b></p><p>Taken from "Human meniscus cells express hypoxia inducible factor-1α and increased SOX9 in response to low oxygen tension in cell aggregate culture"</p><p>http://arthritis-research.com/content/9/4/R69</p><p>Arthritis Research & Therapy 2007;9(4):R69-R69.</p><p>Published online 18 Jul 2007</p><p>PMCID:PMC2206369.</p><p></p> Hypoxia inducible factor (HIF)-1α gene expression in the outer (O; white bars) and inner (I; black bars) region of meniscus tissue from three donors (= 3). Prolyl 4-hydroxylase (P4H)α(I) and HIF prolyl-hydroxylase (PHD)2 gene expression in the outer (O; white bars) and inner (I; black bars) region of meniscus tissue (= 3). Values are expressed as mean ± standard deviation. *< 0.05, **< 0.01 (by Student's unpaired -test) in inner versus outer region of the meniscus matched against the same donor

Collagen types I and II, and SOX9 gene expression in human meniscus cells

<p><b>Copyright information:</b></p><p>Taken from "Human meniscus cells express hypoxia inducible factor-1α and increased SOX9 in response to low oxygen tension in cell aggregate culture"</p><p>http://arthritis-research.com/content/9/4/R69</p><p>Arthritis Research & Therapy 2007;9(4):R69-R69.</p><p>Published online 18 Jul 2007</p><p>PMCID:PMC2206369.</p><p></p> Collagen type I alpha 2 (COL1A2) gene expression levels of cell aggregate cultures of (panel a) outer and (panel b) inner meniscus cells (= 6) in 5% oxygen versus 20% oxygen. Collagen type II alpha 1 (COL2A1) gene expression in aggregate cultures of (panel c) outer and (panel d) inner meniscus cells (= 6) in 5% oxygen versus 20% oxygen. SOX9 (Sry-related HMG box-9) gene expression levels of aggregate cultures of (panel e) outer and (panel f) inner meniscus cells (= 6) in 5% oxygen versus 20% oxygen. *< 0.05, **< 0.01 (by Student's unpaired -test)

YC-1 mediated inhibition of hypoxic induction of P4H in meniscus cell aggregates

<p><b>Copyright information:</b></p><p>Taken from "Human meniscus cells express hypoxia inducible factor-1α and increased SOX9 in response to low oxygen tension in cell aggregate culture"</p><p>http://arthritis-research.com/content/9/4/R69</p><p>Arthritis Research & Therapy 2007;9(4):R69-R69.</p><p>Published online 18 Jul 2007</p><p>PMCID:PMC2206369.</p><p></p> Ratio of hypoxia (5% oxygen) induced prolyl 4-hydroxylase (P4H)α(I) gene expression (post-normalization to β-actin) to P4Hα(I) gene expression (post-normalization to β-actin) in 20% oxygen in monolayer culture of meniscus cells in the presence and absence of the hypoxia inducible factor-1α inhibitor YC-1. Data are expressed as mean ± standard deviation (= 3)

Expression of thrombospondin-1 (TSP-1) and signal-regulatory protein-alpha (SIRPα) by human articular chondrocytes and molecular associations of CD47/IAP

Protein extracts from primary cultures of human articular chondrocytes were separated by SDS-PAGE and immunoblotted with antibodies against TSP-1 and SIRPα. For TSP-1, a 6% gel under reducing conditions was used, whereas for SIRPα, an 8% gel under non-reducing conditions was used. Protein extracts from primary cultures of human articular chondrocytes were immunoprecipitated with antibodies against CD47/IAP and immunoblotted for TSP-1, SIRPα, or α5 integrin. TSP-1, SIRPα, and α5 integrin are identified in non-immunoprecipitated extracts, but only TSP-1 and α5 integrin co-immunoprecipitate with CD47/IAP. IP, immunoprecipitated protein extracts; MW, molecular weight; N, normal human articular chondrocytes; Non IP, non-immunoprecipitated protein extracts; OA, osteoarthritic human articular chondrocytes.<p><b>Copyright information:</b></p><p>Taken from "CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an model"</p><p>http://arthritis-research.com/content/10/1/R4</p><p>Arthritis Research & Therapy 2008;10(1):R4-R4.</p><p>Published online 10 Jan 2008</p><p>PMCID:PMC2374443.</p><p></p