1 research outputs found
Probing Cell-Surface Carbohydrate Binding Proteins with Dual-Modal Glycan-Conjugated Nanoparticles
Dual-modal
fluorescent magnetic glyconanoparticles have been prepared and shown
to be powerful in probing lectins displayed on pathogenic and mammalian
cell surfaces. Blood group H1- and Le<sup>b</sup>-conjugated nanoparticles
were found to bind to BabA displaying <i>Helicobacter pylori</i>, and Le<sup>a</sup>- and Le<sup>b</sup>-modified nanoparticles are
both recognized by and internalized into DC-SIGN and SIGN-R1 expressing
mammalian cells via lectin-mediated endocytosis. In addition, glyconanoparticles
block adhesion of <i>H. pylori</i> to mammalian cells, suggesting
that they can serve as inhibitors of infection of host cells by this
pathogen. It has been also shown that owing to their magnetic properties,
glyconanoparticles are useful tools to enrich lectin expressing cells.
The combined results indicate that dual-modal glyconanoparticles are
biocompatible and that they can be employed in lectin-associated biological
studies and biomedical applications