The Role of the Prodomain in the Folding of Carboxypeptidase A1

Carboxypeptidase A1 (CPA1) is an enzyme utilized in digestion. CPA1 contains a prodomain region, which must be cleaved for enzymatic function. The prodomain region has been hypothesized to be necessary for structural stability, as previous studies were unable to produce a functional CPA1 protein without the prodomain region. However, the recent discovery of a related enzyme, Carboxypeptidase 0 (CP0), which does not have a prodomain yet functions enzymatically, challenges this assertion. To determine if the prodomain is essential to the folding of CPA1, an HA-tag was added for detection, and the prodomain was deleted via PCR. A Western blot was performed to determine expression and enzyme assays were conducted to determine function. CPA1 without a prodomain was detected on the Western blot, but did not display activity in the enzyme assays. This indicates that CPA1 without a prodomain folds adequately enough to be expressed but is nonfunctional. Therefore, the prodomain region appears to be necessary for CPA1 structure and functionality

The Student Movement Volume 105 Issue 9: Zooming Away: COVID Alert Sends AU Remote

NEWS AUSA Hosts AU\u27s Got Talent, Caralynn Chan AU Says an Early Goodbye to Face-to-Face Semester, Joelle Kim Tenth Annual Honors Church Service Reflects on Christian Stewardship, Elianna Srikureja PULSE Election Results, Interviewed by Masy Domecillo Turkey is Overrated: A Korean Variation on Thanksgiving Dinner, Jessica Rim HUMANS Home for the Holidays: How AU Students Celebrate Christmans, Interviewed by Celeste Richardson Reflecting on Fall Semester with Vice President Frances Faehner, Alyssa Henriquez ARTS & ENTERTAINMENT Tribute to Alex Trebek, Hannah Cruse AUdio Christmas EP is TBD, Interviewed by Megan Napod IEDAS Election Aftermath, Lyle Goulbourne On Healing, Adoniah Simonhttps://digitalcommons.andrews.edu/sm-105/1009/thumbnail.jp

The Student Movement Volume 105 Issue 15: SASA\u27s Saris Shine at Cultural Celebration

NEWS SASA Hosts Once Upon a Time in Bollywood , Caralynn Chan Vaccinated: Andrews Students Receive Fist Dose of COVID-19 Vaccine, Taylor Uphus PULSE AAPI Issues on Campus: A Dialogue, Jessica Rim Student Features: The Story Behind the Car, Interviews by Wambui Karanja Summer Plans: STEM Majors, Interviews by Masy Domecillo HUMANS A Quarter Century of Research, Interviewed by Alyssa Henriquez An Interview with Taylor Biek: Next Year\u27s AUSA Social VP, Interviewed by Abigail Lee Makarios Easter Passion Play, Interviewed by Ben Lee ARTS & ENTERTAINMENT April Current Favorite Songs, Hannah Cruse Celebrating National Poetry Month, Alannah Tjhatra Creative Spotlight: Karen Garcia, Interviewed by Megan Napod Signal Boost: Rookie Historian Goo Hae-ryung, Hannah Cruse IDEAS A Birthday Boy\u27s Reflections: What I\u27m Learning Now, Adoniah Simon Biden\u27s Job Plan: The Latest Example of Government Investment in Our Future, Lyle Goulbourne THE LAST WORD My (Last) Last Word, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1019/thumbnail.jp

The Student Movement Volume 105 Issue 7: Putting the Finishing Touches on Fall Semester

NEWS Andrews Releases Spring 2021 Opening Plan, Amanda Cho Creating Visions for the Future, Joelle Kim Semester Reflections: Living During a Global Pandemic, Taylor Uphus PULSE The Best Drive-Through Meals, Masy Domecillo Reviewing Leaving the Shadowland of Stress, Depression, and Anxiety , Jessica Rim Study Tips: Re-Examining Routine, Wambui Karanja HUMANS Christmas vs. Thanksgiving, Interviewed by Abigail Lee Interview with Dongchan Kim, AUSA Executive Vice President, Interviewd by Ben Lee Studying Abroad in Argentina: An Interview with Lisiane Umuhire, Interviewed by TJ Hunter ARTS & ENTERTAINMENT Creative Spotlight: Ivan Rachath, Interviewed by Megan Napod Elementary, Hannah Cruse It\u27s Beginning to Sound A Lot Like Christmas!, Megan Napod IDEAS The Good, Kyara Samuels The Power of Resource, Evin N. Musgrove LAST WORD Fallen Titans: Remembering Alex Trebek and Rabbi Jonathan Sacks, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1008/thumbnail.jp

The Student Movement Volume 105 Issue 13: AU Students Speak out Against Anti-AAPI Violence

NEWS James White Library Offers Useful Resources to Students, Amanda Cho The Office of Diversity and Inclusion and the Center for Faith Engagement Host Asian American and Pacific Islander Forum, Jenae Rogers The Office of Research and Creative Scholarship Hosts the Undergraduate Research and Honors Scholar Symposium, Caralynn Chan PULSE My Experience with Robinhood, Matthew Jarrard Nature Spots on Campus, Gloria Oh Pilipino Culture Night, Interviews by Masy Domecillo HUMANS Interview with T Bruggemann, Terika Williams Interviewing Andrews University\u27s AAPI Students on Allyship, Interviewed by Abigail Lee Senior Spotlight: Hailey Kim, Interviewed by Celeste Richardson ARTS & ENTERTAINMENT Creative Spotlight: Marcel Mattox, Interviewed by Joshua Deonarine AAPI Documentaries You Need to Watch, Megan Napod Asian Women, Courtesy of Hollywood, Hannah Cruse IDEAS Flying Sparks: The Implications of the Rise in AAPI Hate, Alannah Tjhatra The Three P\u27s of Perspective, Kyara Samuels THE LAST WORD Respecting Apoliticism, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1017/thumbnail.jp

The Student Movement Volume 105 Issue 12: Driving-In to the Spider-Verse: AUSA Hosts Arachnid Movie Night

NEWS Andrews University Wind Symphony Holds Vespers, Jenae Rogers One Year Later: Reflecting on the Changes at Andrews University Since COVID-19, Taylor Uphus The Center for Faith Engagement Hosts World Changers Week, Terika Williams PULSE COVID-safe Adventures, Interview by HeeYun Oh The Vaccine, Masy Domecillo What is St. Patrick\u27s Day?, Interviews by Wambui Karanja HUMANS AUSA Art Director Interview, Interviewed by Brandi Seawood Examining the Myanmar Crisis with Rebecca Fanai, Interviewed by Fitz-Ear McKenzie II The Day Our World Shut Down, Interviews by Terika Williams ARTS & ENTERTAINMENT Creative Spotlight: Karenna Lee, Interviewed by Megan Napod Current Favorite Women in the Arts, Kaela McFadden Cuties: Social Value vs. Social Harm, Hannah Cruse IDEAS Allow the Good Things, Evin N. Musgrove What You Can Do: Allyship for the Everyday, Abigail Lee THE LAST WORD Reaching for the Impossible, Kurt Kuhlmanhttps://digitalcommons.andrews.edu/sm-105/1016/thumbnail.jp

The Student Movement Volume 105 Issue 6: AU Infused with New Cultural Perspectives

NEWS Andrews University Wind Symphony Performs Songs of the Sea Concert, Jenae Rogers Dr. Marilyn McEntyre Speaks at John O. Walker Lecture, Taylor Uphus Students Worship Together at Infusion Vespers, Caralynn Chan PULSE Interest and Apathy Towards Politics, Wambui Karanja Memories of Chuseok: Celebrating Community, Jessica Rim Pumpkin Spice: Deconstructed, Masy Domecillo HUMANS Explaining the Letter: Talking with Kelli Coffen, Interviewed by Abigail Lee Holly Sharp, AFIA Cultural VP, on Filipino American History Month, Interviewed by Terika Williams Thoughts on the Last Presidential Debate, Interviewed by Alyssa Henriquez ARTS & ENTERTAINMENT Another Fall Playlist!, Created by Evin N. Musgrove Artist Spotlight: Courtney Saunders, Interviewed by Pearl Parker Unorthodox, Hannah Cruse IDEAS Get Your Flu Shot! Now More Than Ever, Sung Been Han Just This Once , Evin N. Musgrove LAST WORD Apoliticism in Election Season, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1006/thumbnail.jp

The Student Movement Volume 105 Issue 10: One Dose Down, One to Go!: President Luxton Vaccinated

NEWS Andrews University Holds Employee Awards Virtual Celebration, Jenae Rogers New Life Hosts Black Like Me: Growing Up Black, Caralynn Chan Texas Winter Storm Impacts Families of Students at Andrews, Taylor Uphus The Red Cross Blood Drive Reveals Growing Shortage in Donor Participation, Amanda Cho PULSE Favorite Meals: Café Edition, Interviewed by Alec Bofetiado The Wellness Column: Decluttering your Room and Mind, HeeYun Oh The Wellness Column: Decluttering Your Room and Mind, Jessica Rim HUMANS AUSA Candidate Interviews, Interviewed by Abigail Lee Freshman Spotlight: Rekha Isaac, Interviewed by Celeste Richardson Trusting the Science, Interviewed by Pearl Parker ARTS & ENTERTAINMENT Creative Spotlight: Karla Torres, Interviewed by Megan Napod Current Favorites: March, Megan Napod Signal Boost: Canvas, Hannah Cruse IDEAS A Woman and Her God, Evin N. Musgrove COVID-19 Updates: The Variants, the Vaccines, and Other News, Sung Been Han Parler, QAnon, and the Freedom of Speech: What Should be Done?, Abigail Lee THE LAST WORD Taking Stock, Masy Domecillohttps://digitalcommons.andrews.edu/sm-105/1013/thumbnail.jp

The Student Movement Volume 105 Issue 2: Clubs Fare Well: Clubs Preserve Community During COVID

NEWS Clubs, Clubs, Clubs Galore, Taylor Uphus The Andreasen Center for Wellness Reopening, Amanda Cho Danielle Pilgrim Delivers Inspiring Message, Terika Williams PULSE Planning University Club Events Amidst a Pandemic, Masy Domecillo Research at the Science Complex During COVID-19, Jessica Rim Socializing During a Pandemic: Freshmen Perspectives, Interviewed by Wambui Karanja HUMANS BSCF Club Spotlight, Interviewed by TJ Hunter Distance Learning Student Interview, Sydney Saint-Jean Meet Lamson Hall\u27s Newest Dean, Interviewed by Pearl Parker Spiritual Leadership During COVID-19, Interviewed by Terika Williams ARTS & ENTERTAINMENT More Apples to Byte, Hannah Cruse Playing Out the Pandemic: Our Orchestras Dealing with COVID, Megan Napod Signal Boost: The Domino Effect, Pearl Parker Virtual Game Night: A Virtual Reality, Joshua Deonarine IDEAS How Climate Change is Fueling the West Coast Fires, Lyle Goulbourne Ideas That Heal Us: So You Think You\u27re Self-Aware, Evin N. Musgrove THE LAST WORD What am I Applying for & Who am I?, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1002/thumbnail.jp

The Student Movement Volume 105 Issue 1: Back with a Bang

NEWS Suicide Awareness Month in the Chaos of 2020, Kavya Mohanram AUSA Lighthouse Vespers: The Gospel in Motion, Caralynn Chan IDEAS A Summer Recap, Alannah Tjhatra How to Survive a Pandemic, Solana Campbell Writing Cards to Senior Citizens, Sung Been Han PULSE Change Day at Michiana Fil-Am: Dual Perspectives from a Student and an Organizer, Masy Domecillo Honors Agape Feast, Jessica Rim HUMANS Chef Linda Brinegar, Interviewed by Alyssa Henriquez Summer During a Global Pandemic, Interviewed by Ben Lee Teaching During COVID-19, Interviewed by Celeste Richardson The Wellness Center Reopening, Interviewed by Terika Williams ARTS & ENTERTAINMENT A Tribute to Chadwick Boseman, Megan Napod Corn and Watermelon for Everyone!, Megan Napod Local Hotspots: (but not the coronavirus kind), Hannah Cruse Radiant Recitals, Isabella Koh THE LAST WORD Where is Home?, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1001/thumbnail.jp