Number of CD34+ cells before freezing and after thawing and lyophilization.

<p>Results are from three different experiments (i.e. different donors); each individual number represents a different sample.</p

Viability percentage of MNC derived from HUCB after freeze thawing with one of the following solutions: IMT-2, EGCG solution, Trehalose solution.

<p>The samples were either immediately evaluated or underwent a washing process and then evaluated again. The data is presented as the mean±SE, different letters represent statistical differences between groups (p<0.05). The means were calculated as the number of viable cells after thawing divided by the number of viable cells before freezing. (N = 48).</p

Viability percentage of MNC derived from HUCB after freeze drying with one of the following solutions: IMT-2, EGCG solution, Trehalose solution.

<p>The samples were either immediately evaluated or underwent a washing process and then evaluated again. The data is presented as the mean±SE, different letters represent statistical differences between groups (p<0.05). The means were calculated as the number of viable cells after thawing divided by the number of viable cells before freezing. (N = 48).</p

Percentage membrane integrity after freeze-thawing and freeze-drying with different concentrations of EGCG.

<p>This experiment was performed on HUCB units from three different donors, in duplicate (n = 60). Data are presented as mean±SE.</p

Percentage membrane integrity after freezing at different ice interface velocities.

<p>The solution used in this experiment was 12.5% (w/v) HSA and 0.1 M trehalose in PBS (Ca²⁺- and Mg²⁺-free). This experiment was performed on HUCB units from three different donors, in triplicate (n = 54). Data are presented as mean±SE.</p

Photographs showing colonies formed after lyophilization and rehydration with DDW of samples frozen in IMT-2 solution.

<p>Photographs showing colonies formed after lyophilization and rehydration with DDW of samples frozen in IMT-2 solution.</p

The number of colonies grown, before freezing and after thawing and rehydration.

<p>Results are from three different experiments (i.e. different donors); each individual number represents a different sample and the colony type is given in brackets (erythrocytic- E/BFU; granulocytic – GM).</p

Percentage membrane integrity after freeze-thawing and freeze-drying in different solutions.

<div><p>All solutions are based on PBS (Ca²⁺- and Mg²⁺-free). This experiment was performed on HUCB units from three different donors, in duplicate (n = 60). Data are presented as mean±SE.</p> <p>donors, in duplicate (n = 60). Data are presented as mean±SE.</p></div

A photo of dry MNCs derived from HUCB after freeze-drying, before (right) and after (left) rehydration with DDW.

<p>A photo of dry MNCs derived from HUCB after freeze-drying, before (right) and after (left) rehydration with DDW.</p