Investigating the Role of Protein Phosphatase 2A in the Salt Stress Response in Arabidopsis thaliana

Protein Phosphatase 2A (PP2A) is a ubiquitous enzyme in eukaryotes that regulates a large array of cellular signaling processes. PP2A is composed of three subunits: catalytic C subunit, regulatory B subunit, and scaffolding/regulatory A subunit. In Arabidopsis thaliana, the A subunit has three isoforms - A1, A2, and A3 - that are highly conserved at the protein level, indicating that these proteins may be functionally interchangeable. In comparison to wildtype plants, seedlings with a mutation in the A1 gene have roots with obvious root cell file rotation or twisting under conditions of moderate salt stress. Twisted root cells result in a characteristic root curling phenotype when seedlings are grown on the surface of vertically-oriented agar plates. Mutations in the A2 and A3 genes do not result in any observable root phenotype, even though all three A subunit isoforms are expressed in roots. To test the hypothesis that differences in expression may be responsible for mutant phenotype variation, hybrid genes were constructed containing promoters from one subunit and coding regions of a different subunit, then transformed into a1mutant Arabidopsis to test for complementation. These tests showed that a1 mutants transformed with transgenes containing A1 or A3 promoters, regardless of coding region, had their mutant phenotype corrected, while the phenotypes of a1 mutants transformed with a2 promoters were only partially corrected. This supports the hypothesis that a deficit in the total A subunit pool, rather than subunit specificity, is a major contributor to the a1 mutant phenotype

Lineage-tracing methods and the kidney

The kidney is a complex organ with over 30 different cell types, and understanding the lineage relationships between these cells is challenging. During nephrogenesis, a central question is how the coordinated morphogenesis, growth, and differentiation of distinct cell types leads to development of a functional organ. In mature kidney, understanding cell division and fate during injury, regeneration and aging are critical topics for understanding disease. Genetic lineage tracing offers a powerful tool to decipher cellular hierarchies in both development and disease because it allows the progeny of a single cell, or group of cells, to be tracked unambiguously. Recent advances in this field include the use of inducible recombinases, multicolor reporters, and mosaic analysis. In this review, we discuss lineage-tracing methods focusing on the mouse model system and consider the impact of these methods on our understanding of kidney biology and prospects for future application

Achieving product quality comparability while making cell culture process changes

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Achieving product quality targets while maintaining high titer in CHO cell culture processes

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The Relationship Between Intensity of Fitness Tracker Usage and Motivation

Many college students engage in unhealthy behaviors which may contribute to diseases. Additionally, some students may lack motivation to rectify these behaviors to improve their long-term health. This study assessed the relationship between intensity of fitness tracker usage and motivation among health professions students at a large, public Midwestern university. This non-experimental, correlational study used convenience sampling and a theoretical framework based on Bandura’s Social Cognitive Theory. Online recruitment announcements were emailed and included links to the consent form and survey. Motivation was measured with Deci and Ryan’s modified self-determination tool. Intensity of tracker usage was measured using a scale of questions regarding various tracker functions. The data was analyzed with Pearson’s coefficient correlations and no significant relationships between intensity of tracker usage and (a) intrinsic motivation, (b) extrinsic motivation, and (c) prosocial behaviors were found

Evaluation of anti-Erysipelothrix rhusiopathiae IgG response in bottlenose dolphins (Tursiops truncatus) to a commercial pig vaccine

Erysipelothrix rhusiopathiae is the causative agent of erysipeloid in humans and of erysipelas in various animals, including bottlenose dolphins Tursiops truncatus, in which an infection has the potential to cause peracute septicemia and death. The purpose of this study was to evaluate the efficacy of using an off-label porcine (ER BAC PLUS®, Zoetis) E. rhusiopathiae bactrin in a bottlenose dolphin vaccination program by determining the anti-E. rhusiopathiae antibody levels in vaccinated dolphins over a 10 yr period. Serum samples (n = 88) were analyzed using a modified fluorescent microbead immunoassay from 54 dolphins, including 3 individuals with no history of vaccination and 51 dolphins with an average of 5 vaccinations, 3 of which had previously recovered from a natural E. rhusiopathiae infection. A mean 311-fold increase in the immunoglobulin G (IgG) antibody index was measured in a subsample of 10 dolphins 14 d after the first booster vaccination. Serum IgG antibody titers were influenced by number of vaccines received (r2 = 0.47, p < 0.05) but not by age, gender, history of natural infection, adverse vaccine reaction, vaccination interval or time since last vaccination. The commercial pig bacterin was deemed effective in generating humoral immunity against E. rhusiopathiae in dolphins. However, since the probability of an adverse reaction toward the vaccine was moderately correlated (p = 0.07, r2 = 0.1) with number of vaccines administered, more research is needed to determine the optimal vaccination interval.This article is published as Nollens, Hendrik H., Luis G. Giménez-Lirola, Todd R. Robeck, Todd L. Schmitt, Stacy DiRocco, and Tanja Opriessnig. "Evaluation of anti-Erysipelothrix rhusiopathiae IgG response in bottlenose dolphins Tursiops truncatus to a commercial pig vaccine." Diseases of Aquatic Organisms 121, no. 3 (2016): 249-256. DOI: 10.3354/dao03061. Copyright 2016 Inter-Research. Posted with permission