The study of essential oil composition of Matricaria chamomilla in Khouzestan

Background and aims: Matricaria chamomilla is an important medicinal plant that widely is used in pharmaceuticals and food industries. The present study was aimed to evaluate the essential oil composition of samples of Matricaria chamomilla collected from different regions of Khouzestan. Methods: In this experimental study, samples of Matricaria chamomilla were collected from different regions of Khuzestan at full flowering stage. The component identification was achieved by the Gas chromatography–mass spectrometry (GC-MS) analysis. Total GC running time was 75 min. The components of essential oil were identified on the basis of comparison of their retention time and mass spectra with published data. Moreover, they were identified by computer matching with Wiley 7n, National Institute of Standards and Technology (NIST) libraries provided with computer controlling the GC-MS system, and Adams book in Centrallab of Agriculture and Natural Resources University of Khuzestan. Results: The main oil compounds of samples of Matricaria chamomilla were α-Pinene, Sabinene, β-Pinene, 1,8-Cineole, Citronellol, Aromadendrone, (E).β-farnesene, Spathulenol, Caryophyllene oxide, α-Bisabolone oxide A, α-Bisabolole oxide B, Chamazolene and α-Bisabolole oxide A, collected from different regions of Khouzestan. Also, α-Bisabolone oxide A, Caryophyllene oxide, Chamazolene and α-Bisabolole oxide A were the highest percentage of the main oil compounds of these samples. Conclusions: The Caryophyllene oxide, α-Bisabolone oxide A, Chamazolene, α-Bisabolole oxide A are dominant components in the essential oil of Matricaria chamomilla in weather and geographical conditions of studied samples and different climates are effective on plant essential oil composition

The measurement of the quercetin of different parts of Tribulus terrestris by HPLC

Background and aims: Tribulus terrestris fruit, leaf, and root have medical effects in the treatment of cancer, viral infections and prevention of cardiovascular diseases. The present study was aimed to evaluate the quercetin flavonoid levels from different parts of the Tribulus terrestris collected from different regions of Khuzestan in 2014. Methods: In this experimental study, four parts of the Tribulus terrestris including; fruits, leaves, stems and roots were collected from different regions of Khuzestan including Shushtar, Mollasani and Andimeshk. The analysis was carried out to compare the chemical profile of the different extracts of Tribulus terrorists using reverse phase HPLC with UV detector. The mobile phase that consisted of phosphoric acid buffer with pH=3 and acetonitrile was used for isocratic elution. The flow rate was adjusted to 1.0 ml/min. The detection wavelength was at 203 nm. All separations were performed at ambient temperature. Results: The results reported that the quercetin flavonoid level were highest in the Andimeshk leaves samples (69.57427 ppm). However, the Andimeshk fruits samples (4.141953 ppm) have the lowest levels of the quercetin flavonoid. Conclusion: Considering the cost effectiveness in extracting compounds from medicinal plants, it is recommended to identify the highest level of the quercetin flavonoid in each region and in each part of the plant

Extraction and measurement of the Quercetin flavonoid of Prosopis farcta in Khouzestan climatic condition

Background and aims: Medicinal plants are a valuable resource for flavonoids extraction. Prosopis fracta is one of the plants with medicinal properties. Prosopis fracta was found in abundance in southern regions in Iran. Coersetin is medicinal substance found in the fruit of this plant. Quercetin is used in treatment of cancer and viral infections. This study was conducted to determine the Quercetin flavonoid in Prosopis fracta samples in different regions of Khuzestan. Methods: The Prosopis fracta fruit collected from different regions of Khuzestan (Susangerd, Ahvaz, Abadan, Mollasani, Behbahan and Ramhormoz). The beads were isolated from the fruit. Shell and the flesh were dried in an Oven. The dried materials were mixed and flavonoids extracted with a suitable solvent. The extract was injected into the High Performance Liquid Chromatography (HPLC) system. Then, the compound, Quercetin quantity and standard peak in each sample have been determined. Results: Based on the results, the Susangerd samples (0.0033 mg/ml) and Abadan (0.0008 mg/ml) have maximum and minimum levels of Quercetin flavonoid, respectively. Conclusions: Quercetin flavonoid extracted from Prosopis farcta fruits of Susangerd is richer than other regions of Khuzestan province. Therefore, it is recommended to use the Prosopis fracta fruit grown in Susangerd for extraction of the Quercetin flavonoid