49 research outputs found
The OPFOS microscopy family: High-resolution optical-sectioning of biomedical specimens
We report on the recently emerging (Laser) Light Sheet based Fluorescence
Microscopy field (LSFM). The techniques used in this field allow to study and
visualize biomedical objects non-destructively in high-resolution through
virtual optical sectioning with sheets of laser light. Fluorescence originating
in the cross section of the sheet and sample is recorded orthogonally with a
camera. In this paper, the first implementation of LSFM to image biomedical
tissue in three dimensions - Orthogonal-Plane Fluorescence Optical Sectioning
microscopy (OPFOS) - is discussed. Since then many similar and derived methods
have surfaced (SPIM, Ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM...) which we
all briefly discuss. All these optical sectioning methods create images showing
histological detail. We illustrate the applicability of LSFM on several
specimen types with application in biomedical and life sciences.Comment: 19 pages, 10 figures, to be published in Anatomical Research
International (Hindawi