314 research outputs found
Requirements for contractility in disordered cytoskeletal bundles
Actomyosin contractility is essential for biological force generation, and is
well understood in highly organized structures such as striated muscle.
Additionally, actomyosin bundles devoid of this organization are known to
contract both in vivo and in vitro, which cannot be described by standard
muscle models. To narrow down the search for possible contraction mechanisms in
these systems, we investigate their microscopic symmetries. We show that
contractile behavior requires non-identical motors that generate large enough
forces to probe the nonlinear elastic behavior of F-actin. This suggests a role
for filament buckling in the contraction of these bundles, consistent with
recent experimental results on reconstituted actomyosin bundles.Comment: 10 pages, 6 figures; text shortene
Depairing critical current achieved in superconducting thin films with through-thickness arrays of artificial pinning centers
Large area arrays of through-thickness nanoscale pores have been milled into
superconducting Nb thin films via a process utilizing anodized aluminum oxide
thin film templates. These pores act as artificial flux pinning centers,
increasing the superconducting critical current, Jc, of the Nb films. By
optimizing the process conditions including anodization time, pore size and
milling time, Jc values approaching and in some cases matching the
Ginzburg-Landau depairing current of 30 MA/cm^2 at 5 K have been achieved - a
Jc enhancement over as-deposited films of more than 50 times. In the field
dependence of Jc, a matching field corresponding to the areal pore density has
also been clearly observed. The effect of back-filling the pores with magnetic
material has then been investigated. While back-filling with Co has been
successfully achieved, the effect of the magnetic material on Jc has been found
to be largely detrimental compared to voids, although a distinct influence of
the magnetic material in producing a hysteretic Jc versus applied field
behavior has been observed. This behavior has been tested for compatibility
with currently proposed models of magnetic pinning and found to be most closely
explained by a model describing the magnetic attraction between the flux
vortices and the magnetic inclusions.Comment: 9 pages, 10 figure
Cochlear Implants in the Workplace: A Nationwide Survey
A nation wide survey of cochlear implant recipients was conducted to study how implants may impact people at work. Using a self-reporting questionnaire, recipients using four cochlear implant designs were surveyed about spoken communication on the job, overall job performance, job satisfaction, confidence in job retention and in seeking new employment, job promotion, and income. Of the implant recipients using their implants at work (106 people), the majority used their implants during all work hours and reported positive changes in their job situations. The survey results suggest that cochlear implants may help in mitigating functional limitations in the workplace resulting from profound hearing loss
Folding of small proteins: A matter of geometry?
We review some of our recent results obtained within the scope of simple
lattice models and Monte Carlo simulations that illustrate the role of native
geometry in the folding kinetics of two state folders.Comment: To appear in Molecular Physic
The interpretation of the field angle dependence of the critical current in defect-engineered superconductors
We apply the vortex path model of critical currents to a comprehensive
analysis of contemporary data on defect-engineered superconductors, showing
that it provides a consistent and detailed interpretation of the experimental
data for a diverse range of materials. We address the question of whether
electron mass anisotropy plays a role of any consequence in determining the
form of this data and conclude that it does not. By abandoning this false
interpretation of the data, we are able to make significant progress in
understanding the real origin of the observed behavior. In particular, we are
able to explain a number of common features in the data including shoulders at
intermediate angles, a uniform response over a wide angular range and the
greater discrimination between individual defect populations at higher fields.
We also correct several misconceptions including the idea that a peak in the
angular dependence of the critical current is a necessary signature of strong
correlated pinning, and conversely that the existence of such a peak implies
the existence of correlated pinning aligned to the particular direction. The
consistency of the vortex path model with the principle of maximum entropy is
introduced.Comment: 14 pages, 7 figure
Exact Solution of the Munoz-Eaton Model for Protein Folding
A transfer-matrix formalism is introduced to evaluate exactly the partition
function of the Munoz-Eaton model, relating the folding kinetics of proteins of
known structure to their thermodynamics and topology. This technique can be
used for a generic protein, for any choice of the energy and entropy
parameters, and in principle allows the model to be used as a first tool to
characterize the dynamics of a protein of known native state and equilibrium
population. Applications to a -hairpin and to protein CI-2, with
comparisons to previous results, are also shown.Comment: 4 pages, 5 figures, RevTeX 4. To be published in Phys. Rev. Let
Conformations of Randomly Linked Polymers
We consider polymers in which M randomly selected pairs of monomers are
restricted to be in contact. Analytical arguments and numerical simulations
show that an ideal (Gaussian) chain of N monomers remains expanded as long as
M<<N; its mean squared end to end distance growing as r^2 ~ M/N. A possible
collapse transition (to a region of order unity) is related to percolation in a
one dimensional model with long--ranged connections. A directed version of the
model is also solved exactly. Based on these results, we conjecture that the
typical size of a self-avoiding polymer is reduced by the links to R >
(N/M)^(nu). The number of links needed to collapse a polymer in three
dimensions thus scales as N^(phi), with (phi) > 0.43.Comment: 6 pages, 3 Postscript figures, LaTe
Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture
The proteins that make up the actin cytoskeleton can self-assemble into a variety of structures. In vitro experiments and coarse-grained simulations have shown that the actin crosslinking proteins α-actinin and fascin segregate into distinct domains in single actin bundles with a molecular size-dependent competition-based mechanism. Here, by encapsulating actin, α-actinin, and fascin in giant unilamellar vesicles (GUVs), we show that physical confinement can cause these proteins to form much more complex structures, including rings and asters at GUV peripheries and centers; the prevalence of different structures depends on GUV size. Strikingly, we found that α-actinin and fascin self-sort into separate domains in the aster structures with actin bundles whose apparent stiffness depends on the ratio of the relative concentrations of α-actinin and fascin. The observed boundary-imposed effect on protein sorting may be a general mechanism for creating emergent structures in biopolymer networks with multiple crosslinkers
Two State Behavior in a Solvable Model of -hairpin folding
Understanding the mechanism of protein secondary structure formation is an
essential part of protein-folding puzzle. Here we describe a simple model for
the formation of the -hairpin, motivated by the fact that folding of a
-hairpin captures much of the basic physics of protein folding. We argue
that the coupling of ``primary'' backbone stiffness and ``secondary'' contact
formation (similar to the coupling between the ``secondary'' and ``tertiary''
structure in globular proteins), caused for example by side-chain packing
regularities, is responsible for producing an all-or-none 2-state
-hairpin formation. We also develop a recursive relation to compute the
phase diagram and single exponential folding/unfolding rate arising via a
dominant transition state.Comment: Revised versio
- …