4 research outputs found
Reaction media of 44I12 cytoplasmic extract incubated with Impranil at 30°C and pH 7.0 during 24h.
<p>Negative control: <i>E</i>. <i>coli</i> host strain Epi100 transformed with the empty pCC1FOS fosmid. For 44I12, the reaction medium, in and out of the tube, is showed, since a solid aggregate could be obtained.</p
Graphical representation of the metagenomic sequence of clone 44I12, and RAST annotation of the identified ORFs.
<p>Red arrow: CE_Ubrb encoding gene.</p
ORFs of interest from the sequenced clones, annotated as putative esterases or proteases.
<p>ORFs of interest from the sequenced clones, annotated as putative esterases or proteases.</p
Phylogenetic tree of sequences characteristic of the first 8 lipolytic families described by Arpigny et Jaeger [51], along with the characterized CE_Ubrb blast hits against the NCBI-NR database and the first blast hits against the ESTHER database.
<p>In red: biochemically characterized enzymes.</p