2 research outputs found
Highly Selective Excited State Intramolecular Proton Transfer (ESIPT)-Based Superoxide Probing
Two novel fluorescent probe conjugates of 2-(benzothiazol-2-yl)-phenol (HBT) enable ratiometric and selective superoxide detection by an established <i>excited state intramolecular proton transfer</i> (ESIPT) mechanism giving a 60-fold intensity increase
Highly Fluorescent and Specific Molecular Probing of (Homo)Cysteine or Superoxide: Biothiol Detection Confirmed in Living Neuronal Cells
Chemodosimetric action in detection of cysteine and homocysteine (310- and 290-fold) and superoxide inputs (336-fold increase) gives significant fluorescence intensity increases. Detection limits of 2.13 Γ 10<sup>β5</sup> M, 1.37 Γ 10<sup>β5</sup> M, and 2.71 Γ 10<sup>β5</sup> M, respectively, are biorelevant and are consistent with βORβ logic gating, demonstrated in intracellular biothiol detection in neuronal cells by way of novel fluorescein derivatization. As per our knowledge, this is the first example of a novel fluorescent probe based on the nucleophilic substitution reaction of biothiols and superoxide through ester cleavage