India: tempered down

On temperaments, communities and conflicts in the river fisheries of Bihar, amidst rigidly persistent caste and class discrimination

Revitalization Of Plant Growth Promoting Rhizobacteria As An Effective Bioinoculant To Enhance The Growth, Production, And Stress Tolerance Of Vegetable Crops. A Short Review

Now environmental pollution is a serious issue. The use of chemical fertilizers continuously can cause soil and water pollution. We know that the soil is a source of indigenous microorganisms.  Among them, the plant-growth-promoting rhizobacteria (PGPR) are promising bioinoculants for vegetable crops that provide sustainable, environmentally friendly ways to increase growth, production, and stress tolerance due to the production of plant-growth-promoting properties (Phosphate solubilization, Indole acetic acid production, etc.). These advantageous bacteria penetrate the rhizosphere, form symbiotic associations, and support nutrient uptake, root growth, and general plant health. By enhancing water and nutrient uptake, controlling osmotic balance, and inducing the plant's immunological response, they also boost vegetable crop stress tolerance. The use of PGPR-based bioinoculants reduces the need for chemical pesticides and fertilizers while minimizing environmental contamination. &nbsp

Numerical simulation of the response of single gap timing RPCs with the space charge effects and Garfield++

In this article, we report the simulated response of timing RPCs of different gas gaps. A 3D Montecarlo code was developed and integrated with Garfield++ to simulate the avalanche processes with space charge effects which allow actual charge and timing spectrums. The results of this study are presented with examples of timing RPCs of gas gaps 0.02 cm and 0.03 cm

Marine lipopeptide Iturin A inhibits Akt mediated GSK3β and FoxO3a signaling and triggers apoptosis in breast cancer

Akt kinase is a critical component of the PI3K/Akt signaling pathway, which is frequently over expressed in human cancers including breast. Therapeutic regimens for inhibiting breast cancer with aberrant Akt activity are essential. Here, we evaluated antitumor effect of a marine bacteria derived lipopeptide ‘Iturin A’ on human breast cancer in vitro and in vivo through disrupting Akt pathway. Proliferation of MDA-MB-231 and MCF-7 breast cancer cells were significantly inhibited by Iturin A and it induced apoptosis as confirmed by increased Sub G1 populations, DNA fragmentation, morphological changes and western blot analysis. Furthermore, Iturin A inhibited EGF induced Akt phosphorylation (Ser473 and Thr308) and its downstream targets GSK3β and FoxO3a. Iturin A inactivated MAPK as well as Akt kinase leading to the translocation of FoxO3a to the nucleus. Gene silencing of Akt in MDA-MB-231 and MCF-7 cells reduced the sensitivity of cancer cells to Iturin A. Interestingly, overexpression of Akt with Akt plasmid in cancer cells caused highly susceptible to induce apoptosis by Iturin A treatment. In a xenograft model, Iturin A inhibited tumor growth with reduced expressions of Ki-67, CD-31, P-Akt, P-GSK3β, P-FoxO3a and P-MAPK. Collectively, these findings imply that Iturin A has potential anticancer effect on breast cancer

Celecoxib alleviates tamoxifen-instigated angiogenic effects by ROS-dependent VEGF/VEGFR2 autocrine signaling

Background: Tamoxifen (TAM) is widely used in the chemotherapy of breast cancer and as a preventive agent against recurrence after surgery. However, extended TAM administration for breast cancer induces increased VEGF levels in patients, promoting new blood vessel formation and thereby limiting its efficacy. Celecoxib (CXB), a selective COX-2 inhibitor, suppresses VEGF gene expression by targeting the VEGF promoter responsible for its inhibitory effect. For this study, we had selected CXB as non-steroidal anti-inflammatory drug in combination with TAM for suppressing VEGF expression and simultaneously reducing doses of both the drugs. Methods: The effects of CXB combined with TAM were examined in two human breast cancer cell lines in culture, MCF7 and MDA-MB-231. Assays of proliferation, apoptosis, angiogenesis, metastasis, cell cycle distribution and receptor signaling were performed. Results: Here, we elucidated how the combination of TAM and CXB at nontoxic doses exerts anti-angiogenic effects by specifically targeting VEGF/VEGFR2 autocrine signaling through ROS generation. At the molecular level, TAM-CXB suppresses VHL-mediated HIF-1α activation, responsible for expression of COX-2, MMP-2 and VEGF. Besides low VEGF levels, TAM-CXB also suppresses VEGFR2 expression, confirmed through quantifying secreted VEGF levels, luciferase and RT-PCR studies. Interestingly, we observed that TAM-CXB was effective in blocking VEGFR2 promoter induced expression and further 2 fold decrease in VEGF levels was observed in combination than TAM alone in both cell lines. Secondly, TAM-CXB regulated VEGFR2 inhibits Src expression, responsible for tumor progression and metastasis. FACS and in vivo enzymatic studies showed significant increase in the reactive oxygen species upon TAM-CXB treatment. Conclusions: Taken together, our experimental results indicate that this additive combination shows promising outcome in anti-metastatic and apoptotic studies. In a line, our preclinical studies evidenced that this additive combination of TAM and CXB is a potential drug candidate for treatment of breast tumors expressing high levels of VEGF and VEGFR2. This ingenious combination might be a better tailored clinical regimen than TAM alone for breast cancer treatment

Sighting of the Greater Adjutant stork Leptoptilos Dubius in Vikramshila Gangetic Dolphin Sanctuary, Bihar, India

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