Multiplication et bulbaison in vitro du glaïeul (Gladiolus grandiflorus Hort.)

In vitro Multiplication and Bulb Formation of (Gladiolus grandiflorus Hort.). In order to set up a new bulb formation and micropropagation of gladiolus in vitro, trials were undertaken using two ornemental gladiolus cultivars 'Peter pears' and 'White friend ship'. The explants used are apical buds with 2 to 3 mm length taken from the cormes. In vitro initiation and multiplication were done for the two cultivars on Murashige and Skoog medium to which 2 mg. l-1 of BA and 0.5 mgl-1 of AIB were added. In vitro root development and bulb formation have been occurred in MS Agar Agar medium with 0.5 mg.l-1 of AIB 30 days later than the same MS medium containing 6% of saccharose and without Agar

Alpha-1 antitrypsin gene polymorphism in Chronic Obstructive Pulmonary Disease (COPD)

Alpha-1-antitrypsin (AAT) plays an important role in the pathogenesis of emphysema, the pathological lesion underlying the majority of the manifestations of Chronic Obstructive Pulmonary Disease (COPD). In this study we tested the hypothesis that common AAT polymorphisms influence the risk of developing COPDs. We investigated PiM1 (Ala213Val), PiM2 (Arg101His), PiM3 (Glu376Asp), PiS (Glu264Val) and PiZ (Glu342Lys) SERPINA1 alleles in 100 COPD patients and 200 healthy controls. No significant differences were observed in allele frequencies between COPD patients and controls, neither did haplotype analysis show significant differences between the two groups. A cross-sectional study revealed no significant relationship between common SERPINA1 polymorphisms (PiM1, PiM2, PiM3) and the emphysematous type of COPD. In addition, FEV1 annual decline, determined during a two-year follow up period, revealed no difference among carriers of the tested polymorphisms

The role of recent admixture in forming the contemporary West Eurasian genomic landscape

Over the past few years, studies of DNA isolated from human fossils and archaeological remains have generated considerable novel insight into the history of our species. Several landmark papers have described the genomes of ancient humans across West Eurasia, demonstrating the presence of large-scale, dynamic population movements over the last 10,000 years, such that ancestry across present-day populations is likely to be a mixture of several ancient groups [1-7]. While these efforts are bringing the details of West Eurasian prehistory into increasing focus, studies aimed at understanding the processes behind the generation of the current West Eurasian genetic landscape have been limited by the number of populations sampled or have been either too regional or global in their outlook [8-11]. Here, using recently described haplotype-based techniques [11], we present the results of a systematic survey of recent admixture history across Western Eurasia and show that admixture is a universal property across almost all groups. Admixture in all regions except North Western Europe involved the influx of genetic material from outside of West Eurasia, which we date to specific time periods. Within Northern, Western, and Central Europe, admixture tended to occur between local groups during the period 300 to 1200 CE. Comparisons of the genetic profiles of West Eurasians before and after admixture show that population movements within the last 1,500 years are likely to have maintained differentiation among groups. Our analysis provides a timeline of the gene flow events that have generated the contemporary genetic landscape of West Eurasia

A new concise representation of frequent patterns through disjunctive search space

International audienc

Etude expérimentale des représentations concises des itemsets fréquents

Rapport technique, Lens (France) - Tunis (Tunisie

An experimental study of concise representations for frequent patterns

Department of Computer Sciences, Faculty of Sciences of Tun is - CRIL Len

Exploring the disjunctive search space towards discovering new exact concise representations for frequent patterns

Rapport technique, Lens (France) - Tunis (Tunisie

Survie et transmission des bactéries à intérêt sanitaire dans les plantes et le sol irrigués par les eaux usées traitées

La plupart des stations d'épuration installées dans les villes tunisiennes rejettent les eaux usées traitées dans l'environnement (mer, cours d'eau...), 15 % seulement de ces eaux sont utilisées dans l'agriculture. La valorisation de ces eaux demeure donc une nécessité particulièrement durant les saisons sèches mais elle est limitée car les eaux usées jouent un rôle de vecteurs des germes entériques susceptibles d'être pathogènes et dont la présence dans l'environnement constitue un risque sanitaire important. Nous avons essayé de voir au cours de ce travail le devenir des cellules de Salmonella typhimurium incubées dans le sol irrigué par les eaux usées ainsi que leur résistance à certains facteurs de stress. Cinq pots renfermant du sol ont été ensemencés par trigonelle et irrigués par les eaux usées contaminées par les bactéries à étudier: Salmonella typhimurium, Aeromonas hydrophila, Pseudomonas aeruginosa, Pseudomonas fluorescens et Escherichia coli. Au cours de la croissance de ces plantes, nous avons suivi l'évolution de différentes souches dans le sol et les plantes. Les résultats obtenus ont permis de voir que seules les deux souches de Pseudomonas passent au niveau des feuilles et que les autres bactéries restent adsorbées au niveau des racines et de la partie inférieure de la tige. Les cellules de salmonelles stressées subissent des modifications biochimiques et perdent certains de leurs caractères clefs. Elles donnent des formes naines capables de traverser les membranes de 0,45 µ de porosité