Determination of antimicrobial susceptibilities on infected urines without isolation

A method is described for the quick determination of the susceptibilities of various unidentified bacteria contained in an aqueous physiological fluid sample, particularly urine, to one or more antibiotics. A bacterial adenosine triphosphate (ATP) assay is carried out after the elimination of non-bacterial ATP to determine whether an infection exists. If an infection does exist, a portion of the sample is further processed, including subjecting parts of the portion to one or more antibiotics. Growth of the bacteria in the parts are determined, again by an ATP assay, to determine whether the unidentified bacteria in the sample are susceptible to the antibiotic or antibiotics under test

The nonlinear Bernstein-Schr\"odinger equation in Economics

In this paper we relate the Equilibrium Assignment Problem (EAP), which is underlying in several economics models, to a system of nonlinear equations that we call the "nonlinear Bernstein-Schr\"odinger system", which is well-known in the linear case, but whose nonlinear extension does not seem to have been studied. We apply this connection to derive an existence result for the EAP, and an efficient computational method.Comment: 8 pages, submitted to Lecture Notes in Computer Scienc

Geometric View of Measurement Errors

The slope of the best fit line from minimizing the sum of the squared oblique errors is the root of a polynomial of degree four. This geometric view of measurement errors is used to give insight into the performance of various slope estimators for the measurement error model including an adjusted fourth moment estimator introduced by Gillard and Iles (2005) to remove the jump discontinuity in the estimator of Copas (1972). The polynomial of degree four is associated with a minimun deviation estimator. A simulation study compares these estimators showing improvement in bias and mean squared error

Application of firefly luciferase assay for adenosine triphosphate (ATP) to antimicrobial drug sensitivity testing

The development of a rapid method for determining microbial susceptibilities to antibiotics using the firefly luciferase assay for adenosine triphosphate (ATP) is documented. The reduction of bacterial ATP by an antimicrobial agent was determined to be a valid measure of drug effect in most cases. The effect of 12 antibiotics on 8 different bacterial species gave a 94 percent correlation with the standard Kirby-Buer-Agar disc diffusion method. A 93 percent correlation was obtained when the ATP assay method was applied directly to 50 urine specimens from patients with urinary tract infections. Urine samples were centrifuged first to that bacterial pellets could be suspended in broth. No primary isolation or subculturing was required. Mixed cultures in which one species was predominant gave accurate results for the most abundant organism. Since the method is based on an increase in bacterial ATP with time, the presence of leukocytes did not interfere with the interpretation of results. Both the incubation procedure and the ATP assays are compatible with automation

Discovery and Characterization of Transiting SuperEarths Using an All-Sky Transit Survey and Follow-up by the James Webb Space Telescope

Doppler and transit surveys are finding extrasolar planets of ever smaller mass and radius, and are now sampling the domain of superEarths (1-3 Earth radii). Recent results from the Doppler surveys suggest that discovery of a transiting superEarth in the habitable zone of a lower main sequence star may be possible. We evaluate the prospects for an all-sky transit survey targeted to the brightest stars, that would find the most favorable cases for photometric and spectroscopic characterization using the James Webb Space Telescope (JWST). We use the proposed Transiting Exoplanet Survey Satellite (TESS) as representative of an all-sky survey. We couple the simulated TESS yield to a sensitivity model for the MIRI and NIRSpec instruments on JWST. We focus on the TESS planets with radii between Earth and Neptune. Our simulations consider secondary eclipse filter photometry using JWST/MIRI, comparing the 11- and 15-micron bands to measure CO2 absorption in superEarths, as well as JWST/NIRSpec spectroscopy of water absorption from 1.7-3.0 microns, and CO2 absorption at 4.3-microns. We project that TESS will discover about eight nearby habitable transiting superEarths. The principal sources of uncertainty in the prospects for JWST characterization of habitable superEarths are superEarth frequency and the nature of superEarth atmospheres. Based on our estimates of these uncertainties, we project that JWST will be able to measure the temperature, and identify molecular absorptions (water, CO2) in one to four nearby habitable TESS superEarths.Comment: accepted for PASP; added discussion and figure for habitable planets; abridged Abstrac

Quantifying structure in networks

We investigate exponential families of random graph distributions as a framework for systematic quantification of structure in networks. In this paper we restrict ourselves to undirected unlabeled graphs. For these graphs, the counts of subgraphs with no more than k links are a sufficient statistics for the exponential families of graphs with interactions between at most k links. In this framework we investigate the dependencies between several observables commonly used to quantify structure in networks, such as the degree distribution, cluster and assortativity coefficients.Comment: 17 pages, 3 figure

SYMPA, a dedicated instrument for Jovian Seismology. II. Real performance and first results

Context. Due to its great mass and its rapid formation, Jupiter has played a crucial role in shaping the Solar System. The knowledge of its internal structure would strongly constrain the solar system formation mechanism. Seismology is the most efficient way to probe directly the internal structure of giant planets. Aims. SYMPA is the first instrument dedicated to the observations of free oscillations of Jupiter. Principles and theoretical performance have been presented in paper I. This second paper describes the data processing method, the real instrumental performance and presents the first results of a Jovian observation run, lead in 2005 at Teide Observatory. Methods. SYMPA is a Fourier transform spectrometer which works at fixed optical path difference. It produces Doppler shift maps of the observed object. Velocity amplitude of Jupiter's oscillations is expected below 60 cm/s. Results Despite light technical defects, the instrument demonstrated to work correctly, being limited only by photon noise, after a careful analysis. A noise level of about 12 cm/s has been reached on a 10-night observation run, with 21 % duty cycle, which is 5 time better than previous similar observations. However, no signal from Jupiter is clearly highlighted.Comment: 13 pages, 26 figure

Solar Magnetic Field Studies Using the 12-Micron Emission Lines. IV. Observations of a Delta-Region Solar Flare

We have recently developed the capability to make solar vector (Stokes IQUV) magnetograms using the infrared line of MgI at 12.32 microns. On 24 April 2001, we obtained a vector magnetic map of solar active region NOAA 9433, fortuitously just prior to the occurrence of an M2 flare. Examination of a sequence of SOHO/MDI magnetograms, and comparison with ground-based H-alpha images, shows that the flare was produced by the cancellation of newly emergent magnetic flux outside of the main sunspot. The very high Zeeman-sensitivity of the 12-micron data allowed us to measure field strengths on a spatial scale which was not directly resolvable. At the flare trigger site, opposite polarity fields of 2700 and 1000 Gauss occurred within a single 2 arc-sec resolution element, as revealed by two resolved Zeeman splittings in a single spectrum. Our results imply an extremely high horizontal field strength gradient (5 G/km) prior to the flare, significantly greater than seen in previous studies. We also find that the magnetic energy of the cancelling fields was more than sufficient to account for the flare's X-ray luminosity.Comment: 14 pages, 5 figures, accepted for Ap.