44 research outputs found
Distribution of study specimens tested using enrichment culture, AMD-LFA and PCR.
<p>Distribution of study specimens tested using enrichment culture, AMD-LFA and PCR.</p
Receiver operating characteristic curve for AMD-LFA and PCR in comparison with enrichment culture.
<p>Receiver operating characteristic curve for AMD-LFA and PCR in comparison with enrichment culture.</p
Performance evaluation of Active Melioidosis Detect-Lateral Flow Assay (AMD-LFA) for diagnosis of melioidosis in endemic settings with limited resources - Fig 1
<p>AMD-LFA test showing a) faint bands and b) strong bands.</p
Diagnostic accuracy of LFA compared with enrichment culture and PCR.
<p>Diagnostic accuracy of LFA compared with enrichment culture and PCR.</p
Kinetics for clearance of CPS from serum.
<p>Mice were intravenously injected with 100 Ī¼g, 20 Ī¼g, or 4 Ī¼g of CPS. Blood samples were collected at the designated time points, and CPS concentrations in serum samples were determined using quantitative sandwich ELISA. The data were best described by a two-parameter monophasic exponential decay model (<i>y = ae</i><sup><i>-bx</i></sup><i>)</i>, where <i>a</i> is the <i>Y</i> intercept and <i>b</i> is the rate constant for clearance. Data shown are mean Ā± standard deviation for five mice per dose per time point. Half-life (t<sub>1/2</sub>) values calculated from the elimination rate constant (<i>b</i>) derived from the model fitting were similar for all three doses of CPS. The results demonstrate that CPS is eliminated rapidly from serum with a half-life of 4 hours, 4.4 hours, or 2.9 hours for the doses of 100 Ī¼g, 20 Ī¼g, or 4 Ī¼g CPS, respectively.</p
<i>In vivo</i> Distribution and Clearance of Purified Capsular Polysaccharide from <i>Burkholderia pseudomallei</i> in a Murine Model
<div><p><i>Burkholderia pseudomallei</i> is the causative agent of melioidosis, a severe infection prominent in northern Australia and Southeast Asia. The āgold standardā for melioidosis diagnosis is bacterial isolation, which takes several days to complete. The resulting delay in diagnosis leads to delayed treatments, which could result in death. In an attempt to develop better methods for early diagnosis of melioidosis, <i>B</i>. <i>pseudomallei</i> capsular polysaccharide (CPS) was identified as an important diagnostic biomarker. A rapid lateral flow immunoassay utilizing CPS-specific monoclonal antibody was developed and tested in endemic regions worldwide. However, the <i>in vivo</i> fate and clearance of CPS has never been thoroughly investigated. Here, we injected mice with purified CPS intravenously and determined CPS concentrations in serum, urine, and major organs at various intervals. The results indicate that CPS is predominantly eliminated through urine and no CPS accumulation occurs in the major organs. Immunoblot analysis demonstrated that intact CPS was excreted through urine. To understand how a large molecule like CPS was eliminated without degradation, a 3-dimenational structure of CPS was modeled. The predicted CPS structure has a rod-like shape with a small diameter that could allow it to flow through the glomerulus of the kidney. CPS clearance was determined using exponential decay models and the corrected Akaike Information Criterion. The results show that CPS has a relatively short serum half-life of 2.9 to 4.4 hours. Therefore, the presence of CPS in the serum and/or urine suggests active melioidosis infection and provides a marker to monitor treatment of melioidosis.</p></div
Organ distribution of CPS.
<p>Mice were intravenously injected with 100 Ī¼g CPS per mouse. Internal organs (lungs, liver, spleen, and kidneys) were collected at various time points post-injection. The organs were homogenized in PBS. CPS amount per organ was determined by quantitative sandwich ELISA. The amount of CPS in blood samples was calculated from the CPS concentration in serum as shown in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0005217#pntd.0005217.g001" target="_blank">Fig 1</a>. Data shown are mean Ā± standard deviation for five mice per time point. The negative values after subtraction of CPS amounts from serum found in each organ were adjusted to zero. The results showed that no significant amount of CPS accumulated in any of the colletced organs.</p
Excreted CPS detection by AMD<sup>ā¢</sup> LFI.
<p>A urine sample from a CPS-treated mouse was serially diluted in mouse control urine to the indicated CPS concentrations. Each concentration of the urine sample then was tested with AMD<sup>ā¢</sup> LFI. The tests were assessed by four examiners in a randomized, semi-blinded manner (panel A), and by using a lateral flow reader (panel B). The results demonstrated that AMD<sup>ā¢</sup> LFI could detect excreted CPS as low as 0.2 ng/mL.</p
Western blot analysis of excreted CPS.
<p>Purified CPS (lane 1) and urine samples (lanes 2ā7) were separated on 7.5% SDS-PAGE gels. All samples including purified CPS were incubated with proteinase K at 60Ā°C for 1 hour, followed by boiling for 10 min before loading on the gels. Lanes 2, 3, and 4 were loaded with control urine spiked with CPS and incubated at 37Ā°C for 30 min, 2 hours, and 8 hours, respectively. Lanes 5, 6, and 7 were urine from CPS-injected mice collected at 30 min, 2 hours, and 8 hours post-injection, respectively. The volume of sample loaded into each lane was adjusted to contain an equal amount of CPS, approximately 1 Ī¼g/lane. After blotting, membranes were probed with mAb 4C4 (1 Ī¼g/mL). Intact CPS was observed in urine samples from CPS-treated mice.</p
Protection in passively immunized mice following i.n. challenge with <i>B. pseudomallei</i> strain 1026b.
<p>BALB/c mice were administered 1 mg of either CPS IgG3 mAb 3C5 or LPS IgG3 mAb 4C7 alone or 1 mg of each mAb in combination by the i.p. route. Intranasal challenge was performed 18 h later with 15 LD<sub>50</sub> of <i>B. pseudomallei</i>. Mice were monitored for 21 days after which gross pathology and spleen cfu were determined on survivors (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035386#pone-0035386-t001" target="_blank">Table 1</a>). Control mice were treated with 1 mg of an irrelevant IgG3 mAb. <i>p</i> values of survival vs. controls are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035386#pone-0035386-t001" target="_blank">Table 1</a>.</p