Spinal cord anaplastic meningioma with extra-neural metastasis in a cat

<div><p>ABSTRACT: Meningiomas are the most frequent neoplasms involving the brain in dogs and cats, and are occasionally observed in the spinal cord. They cause compression of the central nervous system; however, do not infiltrate the neuropile and rarely metastasize to other organs. The present study describes a case of anaplastic spinal meningioma with extra neural metastasis in a 20 years-old cat. Clinically, the feline presented a clinical history of 120 days with paresis of the hind limbs and loss of the tail’s movements, with subsequent death. At necropsy, there was an irregular and soft whitish mass involving the meninges from the lumbar intumescence to the sacral region of the spinal cord. Similar white nodular masses were observed in the lungs, liver and kidneys. Microscopically, both were composed of a poorly limited and infiltrative neoplastic proliferation composed by spindle, round and epithelioid cells, with a high cellular pleomorphism. On IHC, there was a severe immunostaining for vimentin and S100. Histopathologic and IHC analysis are important tools for definitive diagnosis of meningiomas in cats, and differentiation of other common neurologic disorders in these animals.</p></div

Fibrinous pleuropneumonia caused by Pasteurella multocida associated with bovine lymphoma

<div><p>ABSTRACT: In this work, we describe an unusual case of fibrinous pleuropneumonia caused by Pasteurella multocida associated with generalized lymphadenomegaly in a bovine. The animal had a one-month history of generalized superficial lymphadenomegaly that progressed to anorexia and submandibular oedema, resulting in spontaneous death. At necropsy, the parenchyma of the lymph nodes and multiple organs was obliterated by a dense proliferation of round neoplastic cells (lymphoma). Additionally, the neoplasm presented multifocal areas of haemorrhage and necrosis, characteristic of lymphoma. The parietal and visceral pleura and parietal pericardium were enlarged and covered diffusely with large amounts of a yellowish fibrillary material. The lungs were mildly enlarged, non-collapsed, and firm and exhibited interlobular septae that were thickened with a gelatinous material. Histopathological examination showed that the parietal and visceral pleura were enlarged due to a diffuse and severe inflammatory infiltrate composed of degenerate neutrophils associated with severe fibrin deposition, characteristic of fibrinous pleuropneumonia. Pleura and parietal pericardium fragments were cultivated in aerobic and microaerobic microbiological conditions. Round greyish colonies of gram-negative coccobacilli that were shiny and non-haemolytic were observed in sheep blood agar. The biochemical profile was indicative of Pasteurella spp. Molecular identification was performed by partial 16S rRNA amplification following sequencing. Pasteurella multocida was confirmed as the primary bacterium associated with the bovine fibrinous pleuropneumonia. We are able to infer that the lymphoma caused immunodepression, which increased the animal’s susceptibility to atypical infectious microorganisms such as pathogenic P. multocida.</p></div

Association of APEC with HD11 chicken macrophages.

<p>Cells were infected at a MOI of 150 CFU/cell as described in Section 2.4. (A) At 6 h p.i., the association of bacteria with macrophages was visualized by Giemsa staining. Magnification is 1000×. (B) Graph shows percentage of infected cells (grey bars) and number of associated bacteria per cell (black bars) for each strain. Data are mean ± standard deviation of three experiments performed in triplicate. 100–200 cells were counted in each sample.</p

Mean organ lesion scores in chickens infected with APEC MT78 and UEL17.

a<p><i>n</i> = 5 chickens per strain at 24 h p.i.</p>b<p>Organ lesions were scored as described <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041031#pone.0041031-Anto1" target="_blank">[2]</a>: air sacs 0–3; lungs 0–5; heart 0–3; liver 0–2 and spleen 0–1; maximum additive lesion score: 14.</p

TUNEL-positive cells in the lungs of APEC-infected chickens.

<p>Lungs were fixed at 12 h p.i. (A) Clogged parabronchus of MT78-infected chicken, stained with HE; the boxed region is amplified and was stained with anti-O2 antibody in (D), and by TUNEL in (G). (B) Clogged parabronchus of IMT5155-infected chicken, stained with HE; the boxed region is amplified and was stained with anti-O2 antibody in (E), and by TUNEL in (H). (C) Lung section from PBS-inoculated chicken, showing intact parabronchi, open and aerated atria and air capillaries, and absence of TUNEL-positive cells. (F) Parabronchus of UEL17-infected chicken, stained by TUNEL. (I) Parabronchus of IMT5104-infected chicken, stained by TUNEL. All <i>E. coli</i>-infected chickens had TUNEL-positive cells in inflamed lung areas. Scale bars, A, B and C, 200 µm; D–I, 50 µm. PL, parabronchial lumen; A, atria; AC, air capillary; S, interparabronchial septa; BV, blood vessel.</p

Lungs from chickens infected intratracheally with IMT5155 (10⁹ CFU).

<p>Lungs were fixed 24 h p.i.; sections were stained with (A) HE or (B) anti-O2 antibody and counter-stained with hematoxylin, and examined with a light microscope. Inflamed parabronchi coincide with the presence of bacteria, labeled with anti-O2. Scale bars 400 µm. BV, blood vessel.</p

sj-pdf-1-vet-10.1177_03009858231186101 – Supplemental material for Auricular and laryngeal chondritis in nursery and finishing pigs

Supplemental material, sj-pdf-1-vet-10.1177_03009858231186101 for Auricular and laryngeal chondritis in nursery and finishing pigs by Anderson H. Gris, Manoela M. Piva, Claiton I. Schwertz, Ana P. Mori, Camila Saremba, Daniel M. Simon, Luciana Sonne, Saulo P. Pavarini and David Driemeier in Veterinary Pathology</p

The effect of bacterial inactivation on APEC-induced HD11 macrophage apoptosis.

<p>Macrophages were infected with viable bacteria (closed symbols), UV-inactivated bacteria (pink symbols) or UV-inactivated bacteria in the presence of 5 µg/mL polymixin B (open symbols) and caspase activation was analyzed as described in the legend to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041031#pone-0041031-g005" target="_blank">Fig. 5</a>. Open circles, uninfected cells; closed circles, cells irradiated with 0.02 J UV (positive control). For viable bacteria and UV-inactivated bacteria data are from two experiments performed in duplicate; the mean ± standard deviation of <i>V</i>_max for each curve is indicated on the right of the curve. For UV-inactivated bacteria in the presence of polymixin B, data are from one experiment.</p

(A) Hydrolysis of caspase 3/7 substrate by cell extracts from <i>E. coli</i>-infected HD11 macrophages.

<p>Macrophages were infected with MT78 (closed squares), UEL17 (open squares), IMT5155 (closed triangles) or IMT5104 (open triangles) at MOI of 150 CFU per cell. At 6 h p.i., cell extracts were prepared and analyzed using the caspase 3/7 substrate as described in Section 2.5. Open circles, uninfected cells; closed circles, cells irradiated with 0.02 J UV (positive control). Data are representative of at least four experiments performed in duplicate; the mean ± standard deviation of <i>V</i>_max for each curve is indicated on the right of the curve. (B) TUNEL analysis of HD11 cells. Macrophages were treated as in (A), and after 8 h, samples were fixed with formaldehyde (3.7% in PBS) and analyzed for TUNEL-positive cells (yellow).</p

Lungs from chickens infected intratracheally with PBS (<i>upper left</i>) or 10⁹ CFU of APEC MT78 (<i>upper centre</i>), UEL17 (<i>upper right</i>), IMT5155 (<i>lower centre</i>), and A_fecal IMT5104 (<i>lower left</i>).

<p>Lungs were fixed at 12 h p.i.. Lungs from UEL17-infected chicken fixed at 24 h p.i. are also shown (<i>lower right</i>). Sections were stained with HE and examined under a light microscope. The lungs of PBS-inoculated chickens had intact parabronchi, and open and aerated atria and air capillaries. The lungs of <i>E. coli</i>-infected chickens had inflamed areas next to intact areas. Scale bars 200 µm. PB, primary bronchus; SB, secondary bronchus; PL, parabronchial lumen; A, atria; AC, air capillary; S, interparabronchial septa; BV, blood vessel; Cat, cartilaginous plates supporting primary bronchus.</p