Effect of fertilization of winter pea and wheat mixture on pea seed yield

Trogodišnjim istraživanjima (1995. do 1998.g.) utvrđivan je utjecaj učinkovitosti bakterizacije sjemena ozimog graška i prihrane dušikom na broj i masu suhe tvari kvržica, te prinose zrna smjese graška cv. Maksimirski ozimi i pšenice cv. Sana. Prije sjetve izvršena je predsjetvena bakterizacija sjemena graška s sojem Rhizobium leguminosarum bv. viciae iz zbirke Zavoda za Mikrobiologiju Agronomskog fakulteta Sveučilišta u Zagrebu. Ukupno najveći broj kvržica na korijenu graška utvrđen je na bakteriziranoj varijanti 2 (31 kvržica/biljci), kao i masa suhe tvari kvržica (0,160 g/biljci). Prosječni prinosi zrna graška iznosili su od 1993 kg ha-1 (kontrola) do 2426 kg ha-1 (bakterizacija). Prosjećni prinosi zrna pšenice iznosili su od 1896 kg ha-1 (kontrola) do 2960 kg ha-1 (prihrana dušikom). Prinosi zrna ozime smjese graška i pšenice su iznosili od 3889 kg ha-1 (kontrola) do 5207 kg ha-1 (prihrana dušikom). Najveći broj mahuna (12,3) i zrna po biljci graška (44,0) je utvrđen na bakteriziranoj varijanti 2. Najveća masa 1000 zrna (115,7 g) i masa zrna po biljci graška (5,09 g) također je utvrđena na bakteriziranoj varijanti 2.Three year field trials (1995-1998) were carried out to determin the effect of seed winter pea inoculation and nitrogen top-dressing on number and nodule dry weight g/plant of pea root and also on the yield of winter pea cv. Maksimirski ozimi and wheat cv. Sana mixture. Just before sowing the inoculation of pea seeds was performed by the variety of Rhizobium leguminosarum bv. viciae which is part of the microbial collection of the Department of Microbiology at the Faculty of Agriculture University of Zagreb. The highest total nodule number on pea root (31 nodule/plant) was determined on the inoculated variant 2 as well as nodule dry weight (0,160 g/plant). Average yield of winter pea were ranging from 1993 kg ha-1 (control) up to 2426 kg ha-1 (inoculation). Average yield of winter wheat were ranging from 1896 kg ha-1 (control) up to 2960 kg ha-1 (nitrogen top-dressing). Average yield of winter peas in mixture wheat were ranging from 3889 kg ha-1 (control) up to 5207 kg ha-1 (nitrogen top-dressing). The highest number of pods (12,3) and of seeds per plant (44,0) was determined on the inoculated variant 2. The highest weight of 1000 seeds (115,7 g) and weight of seeds per plant (5,09 g) was determined on the inoculated variant 2

Effect of inoculation and nitrogen top-dressing on quality and yields of winter pea in wheat mixture

Dvogodišnjim istraživanjima (1999. do 2001.g.) utvrđivan je utjecaj bakterizacije sjemena ozimog graška i prihrane dušikom na broj i masu suhe tvari kvržica na korijenu graška, te prinos suhe tvari i krmnu vrijednost smjese graška cv. Maksimirski ozimi i pšenice cv. Sana. Prije sjetve izvršena je predsjetvena bakterizacija sjemena graška sojem Rhizobium leguminosarum bv. viciae iz zbirke Zavoda za Mikrobiologiju Agronomskog fakulteta Sveučilišta u Zagrebu. Najveći broj kvržica na korijenu graška utvrđen je na varijanti bakteriziranoj sojem Rhizobium leguminosarum bv. viciae (34 kvržice/biljci), kao i masa suhe tvari kvržica na korijenu graška (0,185 g/biljci). Ukupni prinosi suhe tvari iznosili su od 14.41 t ha-1 (kontrola bez bakterizacije sjemena graška i prihrane dušikom) do 17.04 t ha-1 (bakterizacija sojem Rhizobium leguminosarum bv. viciae). Prinosi sirovih bjelančevina graška u 2001. g. varirali su od 1781 kg ha-1 (prihrana dušikom 2 x 100 kg/ha KAN-a) do 2269 kg ha-1 (bakterizacija), a pšenice od 902 kg ha-1 (kontrola) do 1270 kg ha-1 (prihrana dušikom). Ukupni prinosi sirovih bjelančevina smjese iznosili su od 2812 kg ha-1 (kontrola) do 3294 kg ha-1 (bakterizacija).Two year field trials (1999-2001) were carried out to determine the effect of seed winter pea inoculation and nitrogen top-dressing on number and nodule dry weight g /plant of pea root and also on the dry matter yield and fodder value of winter pea cv. Maksimirski ozimi and wheat cv. Sana mixture. Just before sowing the inoculation of pea seeds was performed by the indigenous variety of Rhizobium leguminosarum bv. viciae which is part of the microbial collection of the Department of Microbiology at the Faculty of Agriculture University of Zagreb. The highest total nodule number on pea root (34 nodule/plant) was determined on the inoculated variant variety of Rhizobium leguminosarum bv. viciae as well as nodule dry weight (0,185 g/plant). Total dry matter yields were ranging from 14,41 t ha-1 (control without inoculated seed of winter pea and nitrogen top-dressing) up to 17,04 t ha-1 (inoculated variety of Rhizobium leguminosarum bv. viciae). Yields of pea crude proteins in 2001 were ranging from 1781 kg ha-1 (nitrogen top-dressing 2 x 100 kg ha-1 27 % ammonium nitrate NH4NO3) up to 2269 kg ha-1 (inoculation) and in wheat, those values were from 902 kg ha-1 (control) up to 1270 kg ha-1 (nitrogen top-dressing). Total crude proteins mixture yields were from 2812 kg ha-1 (control) up to 3294 kg ha-1 (inoculation)

Utjecaj bakterijskih inokulanta na kemijski sastav i fermentaciju silaže lucerne

Managing alfalfa silage in livestock production systems is an important issue in order to maintain the silage quality and achieve maximum profitable production of milk and meat. The aim of this study was to estimate effects of commercial bacterial inoculants on chemical composition and fermentation of alfalfa silage, under field conditions in the commercial dairy farm, during 2017. The silage mass was subdivided into five equal parts (control - silage without inoculant) and silages treated with commercial bacterial inoculants (PIO 1 - Pioneer 11H50, PIO2 - Pioneer 11AFT, SIL - Silko and BON - Bonsilage alfa) all ensiled in microsilos. After 90 days of ensiling, silages were analysed for chemical and nutritional composition and fermentation characteristics. Dry matter and crude protein value were higher, lactic acid and acetic acid value were significantly higher in silage treated with bacterial inoculant PIO1, PIO2, SIL and BON compared to control silage. Contrary, alfalfa silage treated with a bacterial inoculant PIO1, PIO2, SIL and BON had lower values of acid detergent fibre, neutral detergent fibre and pH and significantly lower values butyric acid, alcohols and NH3-N/total nitrogen compared to control silage. Results showed that bacterial inoculant PIO1, PIO2, SIL and BON increases silage quality compared to control silage.alfalfa; chemical composition; fermentation parameters; inoculants; silag

Genotypic Characterisation of Indigenous Rhizobium leguminosarum bv. viciae Field Population in Croatia

Genetic diversity of thirteen Rhizobium legumnosarum bv. viciae strains isolated from different field sites in continental part of Croatia was investigated. All rhizobial isolates were obtained either from plants grown in pots containing soil samples or from field grown plants. The strains were analyzed for DNA polymorphism using two DNA fingerprinting methods - randomly amplified polymorphic DNA (RAPD-PCR) and repetitive extragenomic palindromic- PCR (rep-PCR). Both methods resulted in very similar grouping of strains. Cluster analysis of rep- and RAPD-PCR profi les showed significant differences among Rh. leguminosarum bv. viciae isolates. The highest differences were detected among reference strains and all field isolates revealing considerable genetic diversity of rhizobial field populations. These results suggest the presence of adapted indigenous Rhizobium leguminosarum bv. viciae strains, probably with higher competitive ability, whose symbiotic properties have to be evaluated in further investigations

Effect of inoculation and nitrogen top-dressing on yield and fodder value of winter pea in wheat mixture

Dvogodišnjim istraživanjima (1999. do 2001.g.) utvrđivan je utjecaj učin-kovitosti bakterizacije sjemena ozimog graška i prihrane dušikom na broj i aktivnost kvržica na korijenu graška, te prinos zelene mase, suhe tvari i krmnu vrijednost smjese graška cv. Maksimirski ozimi i pšenice cv. Sana. Prije sjetve izvršena je predsjetvena bakterizacija sjemena graška s autohtonim sojem Rhizobium leguminosarum bv. viciae iz zbirke Zavoda za mikrobiologiju Agronomskog fakulteta Sveučilišta u Zagrebu. U utvrđivanju (25. 04. 2000. i 2001.) ukupni najveći broj kvržica na korijenu graška utvrđen je na bakteriziranoj varijanti (154), kao i aktivni broj kvržica 144. Prosječni prinosi zelene mase smjese iznosili su od 46,3 t ha-1 (kontrola) do 54,7 t ha-1 (bakterizacija). U ukupnom prinosu zelene mase, udio mase graška iznosio je od 28 % (prihrana dušikom) pa do 34 % (kontrola). Ukupni prinosi suhe tvari iznosili su od 11,65 t ha-1 (kontrola) pa do 13,25 t ha-1 (prihrana dušikom). U masi bakterizirane varijante 2 i kontrolne varijante 1 je utvrđen veći udio graška u suhoj tvari (29,6 i 28 %) u odnosu na dušikom prihranjivane varijante 3 (23,8 %) i 4 (22,5 %). Prinosi sirovih bjelančevina graška u 2001. g. varirali su od 196 kg ha-1 (kontrola) do 300 kg ha-1 (bakterizacija), a pšenice od 1 707 kg ha-1 (kontrola) do 2 111 kg ha-1 (prihrana dušikom). Ukupni prinosi sirovih bjelančevina smjese iznosili su od 1 903 kg ha-1 (kontrola) do 2 407 kg ha-1 (prihrana dušikom).Two year field trials (1999-2001) were carried out to determin the effect of seed winter pea inoculation and nitrogen top-dressing on number and activity of pea root nodules and also on the green mass and dry matter yield of winter pea cv. Maksimirski ozimi and wheat cv. Sana mixture. Just before sowing the inoculation of pea seeds was performed by the indigenous variety of Rhizobium leguminosarum bv. viciae which is part of the microbial collection of the Department of Microbiology at the Faculty of Agriculture University of Zagreb. In the investigation period (25th April, 2000 and 2001) the highest total nodule number on pea root was determined on the inoculated variant (154) as well as active nodule 144. Average mixture green mass yield were ranging from 46,3 t ha-1 (control) up to 54,7 t ha-1 (inoculation). Pea mass content in total green mass yield was from 28 % (nitrogen top-dressing) up to 34 % (control). Total dry matter yields were ranging from 11,65 t ha-1 (control) up to 13,25 t ha-1 (nitrogen top-dressing). Inoculated mass variant 2 and control variant 1 had (29,6 % and 28 %) a higher pea mass content in respect to nitrogen top-dressing variants 3 and 4 (23,8 and 22,5 %). Pea crude proteins yields in 2001 were ranging from 196 kg ha-1 (control) up to 300 kg ha-1 (inoculation) and for wheat, those values ranged from 1 707 kg ha-1 (control) up to 2 111 kg ha-1 (nitrogen top-dressing). Total crude proteins mixture yield were from 1 903 kg ha-1 (control) up to 2 407 kg ha-1 (nitrogen top-dressing)

Effect of inoculation and nitrogen top-dressing on yield and fodder value of winter pea in triticale mixture

Dvogodišnjim istraživanjima (1999. do 2001. g.) utvrđivan je utjecaj bakterizacije sjemena ozimog graška kao i utjecaj prihrane dušikom na broj i aktivnost kvržica na korijenu graška, na prinos zelene mase, suhe tvari i na krmnu vrijednost smjese graška cv. Maksimirski ozimi i tritikale cv. Clercal. Prije sjetve izvršena je predsjetvena bakterizacija sjemena graška autohtonim sojem Rhizobium leguminosarum bv. viciae iz zbirke Zavoda za mikrobiologiju Agronomskog fakulteta Sveučilišta u Zagrebu. Najveći ukupni broj kvržica na korijenu graška (141), kao i aktivni broj kvržica (133) utvrđen je na bakteriziranoj varijanti. Prosječni prinosi zelene mase smjese iznosili su od 48,2 t ha-1 (kontrola) do 52,1 t ha-1 (prihrana dušikom). Ukupni prinosi suhe tvari iznosili su od 11,6 t ha-1 (kontrola) do 13,3 t ha-1 (prihrana dušikom). Prinosi sirovih bjelančevina graška u 2001. g. varirali su od 196 kg ha-1 (kontrola) do 333 kg ha-1 (prihrana dušikom), a tritikale od 1 562 kg ha-1 (kontrola) do 2 105 kg ha-1 (prihrana dušikom). Ukupni prinosi sirovih bjelančevina smjese iznosili su od 1 758 kg ha-1 (kontrola) do 2 438 kg ha-1 (prihrana dušikom).Two year field trials (1999-2001) were carried out to determin the effect of seed winter pea inoculation and nitrogen top-dressing on a number and activity of pea root nodules and also on the green mass and dry matter yield of winter pea cv. Maksimirski ozimi and triticale cv. Clercal mixture. Wright before sowing the inoculation of pea seeds was performed by the indigenous variety of Rhizobium leguminosarum bv. viciae which is part of microbial collection of the Department of Microbiology at the Faculty of Agriculture University of Zagreb. The highest total nodule number on pea root (141) was determined on the inoculated variant as well as active nodule (133). Average mixture green mass yield were ranging from 48,2 t ha-1 (control) up to 52,1 t ha-1 (nitrogen top-dressing). Total dry matter yields were ranging from 11,6 t ha-1 (control) up to 13,3 t ha-1 (nitrogen top-dressing). Pea crude proteins yields in 2001 were ranging from 196 kg ha-1 (control) up to 333 kg ha-1 (nitrogen top-dressing) and for triticale, those values were from 1 562 kg ha-1 (control) up to 2 105 kg ha-1 (nitrogen top-dressing). Total crude proteins mixture yield were from 1 758 kg ha-1 (control) up to 2 438 kg ha-1 (nitrogen top-dressing)

Effect of sowing date on yield and quality of high moisture ear and grain production for maize

Namjenska proizvodnja kukuruza (Zea mays L.) za vlažno zrno i klip ima prednosti u usporedbi s proizvodnjom suhog zrna, budući da se mogu uzgajati hibridi dulje vegetacije i zato što nema troškova sušenja. Dvogodišnji pokusi provedeni na pokušalištu Maksimir Agronomskog fakulteta u Zagrebu imali su za cilj istražiti utjecaj naknadnih rokova sjetve na prinos i kvalitetu vlažnog zrna i klipa kukuruza u usporedbi s onim iz optimalnog roka sjetve. Hibridi kukuruza vegetacijskih skupina FAO 200 (PR39K38) i 300 (PR38P05) sijani su unutar optimalnog i dva naknadna roka sjetve i uzgajani u uvjetima intenzivne agrotehnike. Prvi naknadni rok sjetve bio je sredinom svibnja, a drugi početkom lipnja. Prinos zrna i klipa značajno je opao u naknadnim rokovima u usporedbi s optimalnim rokom sjetve, unatoč činjenici da su istraživani hibridi dostigli stadij fiziološke zrelosti prije prvih jesenskih mrazeva. Pad prinosa u naknadnim rokovima sjetve prvenstveno je rezultat manjeg broja zrna na klipu, a dijelom i smanjene mase 1 000 zrna. Oba hibrida ostvarila su sličan prinos klipa, ali je hibrid dulje vegetacije (PR38P05) imao značajno veće prinose zrna od hibrida kraće vegetacije (PR39K38,) jer je potonji hibrid imao značajno manji indeks krunjenja (82,1 %) od prvoga (87,0 %). Rok sjetve i vegetacijska sezona nisu utjecali na sadržaj proteina i masti u zrnu. Hibridi se također nisu razlikovali po sadržaju proteina, dok je PR38P05 imao apsolutno mali, ali značajno veći sadržaj masti u zrnu od PR39K38. Za proizvodnju vlažnog zrna i klipa u naknadnimm rokovima sjetve do prve dekade lipnja mogu se uspješno uzgajati hibridi grupa FAO 200 i 300 uz zadržavanje kvalitete, ali niže prinose, u usporedbi s onim iz optimalnog roka sjetve.High moisture ear and grain production of maize (Zea mays L.) has advantages in comparison with dry grain production because longer maturity hybrids might be grown and there are no grain drying costs. A two year study was carried out at the Faculty of Agriculture experimental field Maksimir to evaluate the effect of delayed sowing dates on yield and quality of high moisture ear and grain production when compared to optimum sowing date. Maize hybrids belonging to the maturity groups FAO 200 (PR39K38) and 300 (PR38P05) were sown at optimum (early May) and two delayed sowing dates (middle May and early June) and grown under intensive cropping system. When compared to optimum sowing date, grain and ear yield significantly decreased with delayed sowing dates despite the fact that grown hybrids reached physiological maturity before the first autumn frosts. These yield reductions at delayed sowing dates were mainly associated with fewer grains per ear, and partly due to lighter 1000-grain weights. Both hybrids resulted in similar ear yield; however, a longer maturity hybrid (PR38P05) had larger grain yields than a shorter-maturity hybrid (PR39K38) because the latter had significantly smaller shelling index (82,1 %) than the former one (87,0 %). Sowing date and growing conditions showed no significant effect on grain protein and oil contents. Hybrids also had similar grain protein content, whereas PR38P05 had absolutely small, but significantly higher grain oil content than PR39K38. Thus, delayed sowing of the maize hybrids of FAO 200 - 300 maturity groups might occur into early June with no effect on grain quality, but with significant yield losses when compared to optimum sowing date

Značenje zrna ozimog graška cv. Maksimirski rani u proizvodnji mlijeka na obiteljskim gospodarstvima

Forage pea (Pisum sativum L.) is gaining importance as a forage legume in the Republic of Croatia. Pea seed contains 20-30 percent of protein, it is utilized without thermal treatment in feeding different types and categories of livestock, and with stable yield it provides an appreciable income per hectare. Two-year field trials (2005-2006) were carried out to determine the effect of winter pea seed inoculation and nitrogen top-dressing on the number and mass (g/plant-1) of root nodules and also on the yield and quality of winter pea cv. Maksimirski rani in a mixture with wheat cv. Sana. Just before sowing, pea seeds were inoculated with the strain Rhizobium leguminosarum bv. viciae 1001 from the microbial collection of the Department of Microbiology, Faculty of Agriculture, University of Zagreb. The highest number of root nodules (43 nodules/plant), as well as the highest nodule mass (0.219 g/plant-1) were determined in the inoculated variant. The highest number of pods (19.0) and seeds per plant (60) were determined in the inoculated variant as well. The highest 1000-seed mass (132 g) and seed mass per plant (7.93 g) were also determined in the inoculated variant. Average pea seed yield ranged from 2949 kg ha-1 (control) up to 3353 kg ha-1 (inoculation). The conclusion of this research is that the highest seed (3353 kg ha-1) and crude protein yields (833 kg ha-1) were obtained with inoculated forage winter pea cv. Maksimirski rani. Seed inoculation of the studied pea cultivar Maksimirski rani with the strain Rhizobium leguminosarum bv. viciae 1001 influenced also higher milk production per hectare compared to the control and the nitrogen top-dressed variant.Krmni grašak (Pisum sativum L.) postaje sve zastupljenija krmna mahunarka na oranicama Republike Hrvatske. Zrno graška sadrži 20-30% bjelančevina, koristi se bez termičke obrade u ishrani različitih vrsta i kategorija stoke, a uz stabilan prinos osigurava značajan dohodak po hektaru. Dvogodišnjim istraživanjima (od 2005. do 2006.) utvrđivan je utjecaj učinkovitosti bakterizacije sjemena ozimog graška cv. Maksimirski rani i prihrane dušikom na broj i masu suhe tvari kvržica na korijenu graška (g/biljka-1), prinose i kvalitetu zrna graška cv. Maksimirski rani u smjesi s pšenicom cv. Sana. Prije sjetve izvršena je predsjetvena bakterizacija sjemena graška sojem Rhizobium leguminosarum bv. viciae 1001 iz zbirke Zavoda za mikrobiologiju Agronomskog fakulteta Sveučilišta u Zagrebu. Ukupno najveći broj kvržica na korijenu graška utvrđen je na bakteriziranoj varijanti (43 kvržica/biljka), kao i masa suhe tvari kvržica (0,219 g/biljka-1). Najveći broj mahuna (19,0) i zrna po biljci graška (60) utvrđen je na bakteriziranoj varijanti. Najveća masa 1000 zrna (132 g) i masa zrna po biljci graška (7,93 g) također je utvrđena na bakteriziranoj varijanti. Prosječni prinosi zrna graška cv. Maksimirski rani iznosili su od 2949 kg ha-1 (kontrola) do 3353 kg ha-1 (bakterizacija). Na temelju ovih istraživanja možemo zaključiti da je najveći prinos zrna (3353 kg ha-1) i sirovih bjelančevina (833 kg ha-1) ostvaren bakterizacijom zrna ozimog graška cv. Maksimirski rani. Bakterizacija sjemena istraživanog kultivara graška Maksimirski rani sojem Rhizobium leguminosarum bv. viciae 1001 utjecala je i na veću proizvodnju mlijeka po hektaru u odnosu na kontrolu i varijantu prihranjivanu dušikom

Značenje zrna ozimog graška cv. Maksimirski rani u proizvodnji mlijeka na obiteljskim gospodarstvima

Forage pea (Pisum sativum L.) is gaining importance as a forage legume in the Republic of Croatia. Pea seed contains 20-30 percent of protein, it is utilized without thermal treatment in feeding different types and categories of livestock, and with stable yield it provides an appreciable income per hectare. Two-year field trials (2005-2006) were carried out to determine the effect of winter pea seed inoculation and nitrogen top-dressing on the number and mass (g/plant-1) of root nodules and also on the yield and quality of winter pea cv. Maksimirski rani in a mixture with wheat cv. Sana. Just before sowing, pea seeds were inoculated with the strain Rhizobium leguminosarum bv. viciae 1001 from the microbial collection of the Department of Microbiology, Faculty of Agriculture, University of Zagreb. The highest number of root nodules (43 nodules/plant), as well as the highest nodule mass (0.219 g/plant-1) were determined in the inoculated variant. The highest number of pods (19.0) and seeds per plant (60) were determined in the inoculated variant as well. The highest 1000-seed mass (132 g) and seed mass per plant (7.93 g) were also determined in the inoculated variant. Average pea seed yield ranged from 2949 kg ha-1 (control) up to 3353 kg ha-1 (inoculation). The conclusion of this research is that the highest seed (3353 kg ha-1) and crude protein yields (833 kg ha-1) were obtained with inoculated forage winter pea cv. Maksimirski rani. Seed inoculation of the studied pea cultivar Maksimirski rani with the strain Rhizobium leguminosarum bv. viciae 1001 influenced also higher milk production per hectare compared to the control and the nitrogen top-dressed variant.Krmni grašak (Pisum sativum L.) postaje sve zastupljenija krmna mahunarka na oranicama Republike Hrvatske. Zrno graška sadrži 20-30% bjelančevina, koristi se bez termičke obrade u ishrani različitih vrsta i kategorija stoke, a uz stabilan prinos osigurava značajan dohodak po hektaru. Dvogodišnjim istraživanjima (od 2005. do 2006.) utvrđivan je utjecaj učinkovitosti bakterizacije sjemena ozimog graška cv. Maksimirski rani i prihrane dušikom na broj i masu suhe tvari kvržica na korijenu graška (g/biljka-1), prinose i kvalitetu zrna graška cv. Maksimirski rani u smjesi s pšenicom cv. Sana. Prije sjetve izvršena je predsjetvena bakterizacija sjemena graška sojem Rhizobium leguminosarum bv. viciae 1001 iz zbirke Zavoda za mikrobiologiju Agronomskog fakulteta Sveučilišta u Zagrebu. Ukupno najveći broj kvržica na korijenu graška utvrđen je na bakteriziranoj varijanti (43 kvržica/biljka), kao i masa suhe tvari kvržica (0,219 g/biljka-1). Najveći broj mahuna (19,0) i zrna po biljci graška (60) utvrđen je na bakteriziranoj varijanti. Najveća masa 1000 zrna (132 g) i masa zrna po biljci graška (7,93 g) također je utvrđena na bakteriziranoj varijanti. Prosječni prinosi zrna graška cv. Maksimirski rani iznosili su od 2949 kg ha-1 (kontrola) do 3353 kg ha-1 (bakterizacija). Na temelju ovih istraživanja možemo zaključiti da je najveći prinos zrna (3353 kg ha-1) i sirovih bjelančevina (833 kg ha-1) ostvaren bakterizacijom zrna ozimog graška cv. Maksimirski rani. Bakterizacija sjemena istraživanog kultivara graška Maksimirski rani sojem Rhizobium leguminosarum bv. viciae 1001 utjecala je i na veću proizvodnju mlijeka po hektaru u odnosu na kontrolu i varijantu prihranjivanu dušikom

INFLUENCE OF BACTERIZATION ON AGRONOMIC TRAITS OF NEW WINTER PEA VARIETY

Cilj istraživanja bio je utvrditi utjecaj bakterizacije sjemena graška s dva soja Rhizobium leguminosarum bv. viciae na broj i masu kvržica na korijenu graška te prinos i kvalitetu kultivara ozimog graška Maksimirski rani i Ozimi šampion u smjesi sa sortom ozime pšenice Sana. Istraživanje je provedeno u vegetacijskoj godini 2009/2010. na pokušalištu Agronomskog fakulteta Sveučilišta u Zagrebu gdje je izvršena bakterizacija sjemena graška sa sojevima R. leguminosarum bv. viciae 1001 kao referentnim i autohtonim sojem R. leguminosarum bv. v viciae KŽ 26. Primjenom bakterizacije oba kultivara dala su bolje rezultate (broj kvržica, broj mahuna, masu 1000 zrna, masu zrna po biljci, sadržaj sirovih bjelančevina, prinos zrna, masu suhe tvari) u odnosu na kontrolnu varijantu. Maksimirski rani dao je bolje rezultate bakterizacijom sa sojem R. lguminosarum bv. viciae 1001, a kultivar Ozimi šampion sa sojem R. leguminosarum bv. viciae KŽ 26. Ozimi šampion bakteriziran sa sojem R. leguminosarum bv. viciae KŽ 26 imao je najveći broj kvržica na korijenu (43), masu suhe tvari kvržica (0,21 g) i masu zrna po biljci (6,7 g), masu 1000 zrna (149 g), najveći sadržaj sirovih bjelančevina u zrnu (260 g/kg) te najveći prinos zrna (3.082 kg/ha) kao i prinos sirovih bjelančevina (802 kg/ha). Kultivar Maksimirski rani bakteriziran sa sojem Rhizobium leguminosarum bv. viceae 1001 imao je najveći broj mahuna (17) i najveći broj zrna po biljci (52). Istraživanjem je potvrđena važnost kompatibilnosti soja za predsjetvenu bakterizaciju i kultivara graška u poljskim uvjetima. Izbor odgovarajućeg soja za određeni kultivar može značajno povećati prinos i kvalitetu uzgajanog kultivara ozimog graška.The aim of this study was to determine the effect of winter pea seed inoculation with two strains of R. leguminosarum bv. viceae on the number and mass of root nodules, as a yield and quality of winter pea cv. Maksimirski rani and Ozimi šampion in a mixture with wheat cv. Sana. The field trial was carried out in the vegetation period of 2009/2010 at the Faculty of Agriculture in Zagreb where pea seeds were inoculated with the strains R. leguminosarum bv. viciae 1001 and bv.viciae KŽ 26. Both inoculated cultivars had a better results compared to the control variant. Pea cultivar Maksimirski rani gives better results when the seed was inoculated with strain 1001, while cultivar Ozimi šampion gives better results when inoculated with strain KŽ 26. Cultivar Ozimi šampion inoculated with R. leguminosarum bv.viceae KŽ 26 had the highest number of nodules (43) and nodules mass per plant (0,21 g), 1000-seed mass (149 g), seed mass per plant (6,7 g), seed yield (3.082 kg/ha), crude protein content in grain (260 g/kg) and the highest crude protein yield (802 kg/ha). Cultivar Maksimirski rani inoculated with R. leguminosarum bv. viceae KŽ 1001 had the highest number of pods per plant (17) and the largest number of seeds per plant (49). This research confirmed the importance of compatibility tests of different strains and pea cultivars under field conditions. Selection of appropriate strains for a particular cultivar can significantly increase yield and quality of winter pea cultivars