Positive species for influenza type A by rRT-PCR and viral isolates obtained in this study.

*<p>Percentage of positive samples obtained by real-time RT-PCR (rRT-PCR) and Virus Isolation (VI) based on the total number of sampled birds.</p><p>N/D: Non-Determined.</p

Phylogenetic trees for internal gene segments NP, M and NS.

<p>All trees were generated in PAUP 4.0b10 using Neighbor-Joining method with 1000 bootstrap replicates (bootstrap values above 70% are shown). Scale bar on the bottom-left indicates number of nucleotide substitutions per site.</p

Phylogenetic trees for N3, N4, and N9 NA genes.

<p>Trees were generated in PAUP 4.0b10 using the Neighbor-Joining method with 1000 bootstrap replicates (bootstrap values above 70% are shown). Scale bar on the bottom-left indicates number of nucleotide substitutions per site.</p

Phylogenetic trees for internal gene segments PB2, PB1, and PA.

<p>All trees were generated in PAUP 4.0b10 using Neighbor-Joining method with 1000 bootstrap replicates (bootstrap values above 70% are shown). Scale bar on the bottom-left indicates number of nucleotide substitutions per site.</p

Location of sample collection sites in the Atlantic (1 and 2) and the Pacific (3, 4, and 5) coasts of Guatemala.

<p>Latitude and longitude of surveillance sites are provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032873#pone-0032873-t001" target="_blank">Table 1</a>.</p

Sites for avian influenza surveillance in wild birds, Guatemala, 2007–2010.

<p>A = Active surveillance (mist nets, live captured birds), K = Hunter-killed.</p

Phylogenetic trees for H5 and H7 HA genes.

<p>Trees were generated in PAUP 4.0b10 using the Neighbor-Joining method with 1000 bootstrap replicates (bootstrap values above 70% are shown). Scale bar on the bottom-left indicates number of nucleotide substitutions per site.</p

Phylogenetic trees for N2 and N8 NA genes.

<p>Trees were generated in PAUP 4.0b10 using Neighbor-Joining method with 1000 bootstrap replicates (bootstrap values above 70% are shown). Scale bar on the bottom-left indicates number of nucleotide substitutions per site.</p

Phylogenetic trees for H3, H8 and H11 HA genes.

<p>Trees were generated in PAUP 4.0b10 using the Neighbor-Joining method with 1000 bootstrap replicates (bootstrap values above 70% are shown). Scale bar on the bottom-left indicates number of nucleotide substitutions per site.</p

Genome constellations of AIVs obtained from wild birds in Guatemala.

<p>Nucleotide percent similarities are shown. The different colors represent different clades supported by bootstrap values >70%. *Isolate CIP049-01 was used as reference to estimate sequence percent similarities.</p