Additional file 1 of Astrocytes of the optic nerve exhibit a region-specific and temporally distinct response to elevated intraocular pressure

Additional file 1: Supplementary Figure 1. (A-B) In-situ hybridization (A, A’) and immunohistochemical staining (B) of the mouse optic nerve for LCN2. We observed very sparse labeling of LCN2 at 7 days after microbead injections (arrowheads). (C-H) Longitudinal sections of the mouse ONH region immunostained for NDUFC2 and COX5B in untreated and microbead injected mice at 30 days. (I, J) Gene set enrichment analysis using the gene ontology (GO) and KEGG database

Additional file 12 of Astrocytes of the optic nerve exhibit a region-specific and temporally distinct response to elevated intraocular pressure

Additional file 12: Supplementary Table 10. Pathways differentiating ONH astrocytes from those in the ONP at 30 days

Additional file 9 of Astrocytes of the optic nerve exhibit a region-specific and temporally distinct response to elevated intraocular pressure

Additional file 9:  Supplementary Table 7. Differentially expressed genes and pathways identified in ONP 30 vs 7 days

Additional file 6 of Astrocytes of the optic nerve exhibit a region-specific and temporally distinct response to elevated intraocular pressure

Additional file 6: Supplementary Table 4. Differentially expressed genes and significant pathways identified in ONH 30 vs 7 days

Additional file 2 of Astrocytes of the optic nerve exhibit a region-specific and temporally distinct response to elevated intraocular pressure

Additional file 2: Supplementary Figure 2. (A) Heatmap showing the per-sample activity score for each transcription factor identified as significantly different between ONH 30 vs 0 days.  While there are significant differences based on the log2 fold change of genes as input, the heatmap showed limited clustering by condition. (B) Table summarizing the known motifs found to be enriched in the transcription start site +/- 2kb region of our input gene list, with a p-value < 0.001.  For each known motif, the corresponding transcription factor is specified. (B) Table summarizing the known motifs found to be enriched in the transcription start site +/- 2kb region of our input gene list, with a p-value < 0.001.  For each known motif, the corresponding transcription factor is specified. (D) In-situ hybridization showing the localization of Cartpt mRNA in the optic nerve of a microbead injected mouse at 30 days. (E) Longitudinal sections of the mouse ONH region showing immunostaining for CARTPT and SOX9 in microbead injected mice at 30 days

Additional file 3 of Astrocytes of the optic nerve exhibit a region-specific and temporally distinct response to elevated intraocular pressure

Additional file 3: Supplementary Table 1. The quantity and quality of RNA and cDNA measured using the Agilent 2100 Bioanalyzer

Additional file 10 of Astrocytes of the optic nerve exhibit a region-specific and temporally distinct response to elevated intraocular pressure

Additional file 10: Supplementary Table 8. Common differentially expressed genes in all three timpoint comparisons in the ONP

Additional file 11 of Astrocytes of the optic nerve exhibit a region-specific and temporally distinct response to elevated intraocular pressure

Additional file 11: Supplementary Table 9. Differentially expressed genes and pathways identified in ONP 30 vs 0 days

Additional file 4 of Astrocytes of the optic nerve exhibit a region-specific and temporally distinct response to elevated intraocular pressure

Additional file 4: Supplementary Table 2. Differential expression analysis using a likelihood ratio test (LRT) focusing on genes in the ONH that differ over time (0, 7 and 30 days) in microbead injected mice

Additional file 5 of Astrocytes of the optic nerve exhibit a region-specific and temporally distinct response to elevated intraocular pressure

Additional file 5: Supplementary Table 3. Differentially expressed genes in pairwise comparisons of ONH 7 vs 0 days