Summary of MOI and <i>F_WS</i> estimates in Burkina Faso, Mali and the combined (West Africa) population.

<p>Summary of MOI and <i>F_WS</i> estimates in Burkina Faso, Mali and the combined (West Africa) population.</p

<i>F_WS</i> against MSP1 MOI, MSP2 MOI, and maximum MOI.

<p><i>F_WS</i> against MSP1 MOI, MSP2 MOI, and maximum MOI.</p

Summary of sequencing coverage per lane across 59 clinical samples.

<p>Data is averaged across all sample lanes within the given ranges of human DNA quantities. On average, each sample was sequenced on 2 lanes.</p

Human DNA percentage post-purification with Lymphoprep plus Plasmodipur against parasitaemia in Burkina Faso.

<p>The dashed line indicates the line of best fit. IRBC = Infected Red Blood Cell.</p

Frequency distribution of human DNA proportions post WBC-depletion.

<p>a) Mali, b) Burkina Faso. Human DNA proportions are presented as the percentage human DNA in the total sample as estimated by quantitative real-time PCR (see methods). The Malian samples (a) represent a range of WBC-depletion methods. All Burkinabe samples (b) were processed using the Lymphoprep plus Plasmodipur approach.</p

Square root of average coverage per base against human DNA proportion for 59 sequenced clinical samples.

<p>Sequence read lengths were either 54 bp (grey spots) or 76 bp (black spots). Each dot represents a sample. Where a sample was sequenced on more than one lane, the average sequence coverage is presented. The median (Square root of average coverage) intra-sample standard deviation was 0.91, and inter-quartile range was 0.70–1.35.</p

Distributions of Illumina Read Alignments against the <i>P. vivax</i>, Human, and <i>P. falciparum</i> Reference Genomes.

<p>*Mixed-species confirmed by PCR. **Poor maturation in <i>ex vivo</i> culture. Note: % <i>P. vivax</i> reads does not include reads which cross-map against <i>P. falciparum.</i></p

Sequence Breadth Distributions across the <i>P. vivax</i> Sal-1 Reference Genome and in Coding and Non-coding Regions.

<p>Samples = 22 independent, pure <i>P. vivax</i> isolates. CDS = coding sequence.</p

Comparative Sequence Depth Distributions between the <i>P. vivax</i> and <i>P. falciparum</i> Genome in THA-009 and DRW-004.

<p>Plots indicate the number of bases (y-axis) with a given sequence depth (x-axis) in different regions of the <i>P. vivax</i> (green) and <i>P. falciparum</i> (blue) genome. Whilst the <i>P. vivax</i> coding and non-coding sequences peak close to the nominal total sequence depth, the <i>P. falciparum</i> coding and non-coding sequences peak at moderately different coverage depths.</p

Sequence Depth Distributions across the <i>P. vivax</i> Sal-1 Reference Genome and in Coding and Non-coding Regions.

<p>Samples = 22 independent, pure <i>P. vivax</i> isolates. CDS = coding sequence.</p