Identifying the amino acids of telomerase that are important for recruitment by TPP1

HonorsCell and Molecular Biology (CMB)University of Michiganhttp://deepblue.lib.umich.edu/bitstream/2027.42/162657/1/oanaed.pd

New Insights Regarding the Potential Health Benefits of Isoflavones

Isoflavones are a class of plant secondary metabolites, with an estrogen‐like structure presenting a plethora of biological activities. The chapter discusses important facts about this class of phytoestrogens, from biosynthesis to the latest research about their health benefits. The following major points discussed are: biosynthesis, regulation, isolation, metabolism and bioavailability, isoflavones in diet and intake, and new insights regarding the therapeutic effect including cancer chemoprevention. The chapter ends with a mini review of own research of the anti‐inflammatory and chemopreventive activity of isoflavonoid genistein alone and incorporated in modern pharmaceutical formulations. The chapter updates the interested researchers in the field with the latest progress regarding potential health benefits of isoflavones

Integrated System of Fed Batch ABE Biosynthesis and Solvent Recovery by Pervaporation

Anaerobic fermentation of glucose substrate by Clostridium acetobutylicum bacteria at 34-39 °C produces butanol, acetone, and ethanol solvents as metabolites. In the case of batch and fed batch bioreactors, the control of butanol concentration in the fermentation broth is recommended for diminishing its inhibitory effect on the bacterial system. Solvent recovery from batch or fed batch bioreactors by using an ultrafiltrationpervaporation system could be an efficient solution to improve the process performances. A mathematical model was developed in order to describe the dynamics of solvent production in an integrated system of fed batch fermentation and solvent recovery. Simulations performed under various operating conditions were used for optimizing the biosynthesis process, i.e., for maximizing the solvent production and minimizing the substrate concentration in the fermentation medium. Initial substrate concentration in the bioreactor, feed flow rate, feed substrate concentration, starting time of feed and ultrafiltration-pervaporation, surface area of ultrafiltration and pervaporation units were selected as process control variables

Flexor Enthesopathy of the Elbow in Three Dogs: Imaging and Surgery

The aim of the study was to describe the radiographic and computed tomography (CT), findings in three dogs with elbow flexor enthesopathy. The study was a clinical one with client-owned dogs. In two dogs, lameness was localized to the elbow by clinical examination. Radiographic examination and CT were performed, and flexor enthesopaty was observed also in the third dog as an incidental finding. Flexorenthesopathy was diagnosed in all three dogs (4 joints) by combining the minimal radiographic changes with specific CT findings. Conservative and surgical treatment were performed. In all joints, any other pathology were excluded. In all three dogs, the elbow condition improved on long-term. Flexor enthesopathy at the medial epicondyle is an unrecognized condition and is a possible cause of elbow lameness in the dog

Cerebrospinal Fluid Findings in Six Dogs with Neurological Disease

The aim of the study was to emphasize the cerebrospinal fluid (CSF) findings in dogs with neurological pathology and to support the clinical relevance of the CSF analysis in patients with neurological diseases. A total of six dogs with pleocytosis, from different breeds were included in this study. Cisternal tap was processed and CSF workup was done in all six. Elevated cell count, increased protein level and cytological findings like lymphocytic, monocytic and mixed pleocytosis were also observed. CSF cytological findings were consistent with inflammatory disorders. CSF analysis is a safe diagnostic tool for detecting central nervous system inflammation in our study, still not confirmatory based solely for definitive diagnosis

Phosphorylation controls autoinhibition of cytoplasmic linker protein-170

Author Posting. © American Society for Cell Biology, 2010. This article is posted here by permission of American Society for Cell Biology for personal use, not for redistribution. The definitive version was published in Molecular Biology of the Cell 21 (2010): 2661-2673, doi:10.1091/mbc.E09-12-1036.Cytoplasmic linker protein (CLIP)-170 is a microtubule (MT) plus-end-tracking protein that regulates MT dynamics and links MT plus ends to different intracellular structures. We have shown previously that intramolecular association between the N and C termini results in autoinhibition of CLIP-170, thus altering its binding to MTs and the dynactin subunit p150Glued (J. Cell Biol. 2004: 166, 1003–1014). In this study, we demonstrate that conformational changes in CLIP-170 are regulated by phosphorylation that enhances the affinity between the N- and C-terminal domains. By using site-directed mutagenesis and phosphoproteomic analysis, we mapped the phosphorylation sites in the third serine-rich region of CLIP-170. A phosphorylation-deficient mutant of CLIP-170 displays an "open" conformation and a higher binding affinity for growing MT ends and p150Glued as compared with nonmutated protein, whereas a phosphomimetic mutant confined to the "folded back" conformation shows decreased MT association and does not interact with p150Glued. We conclude that phosphorylation regulates CLIP-170 conformational changes resulting in its autoinhibition.This work was supported by National Institutes of Health grant GM-25062 (to G.G.B.); Netherlands Organization for Scientific Research grants (to A. A. and N. G.); a Cancer Genomics Centre grant (to J.v.H.); and Presidential Program of Russian Academy of Sciences and RFBP grant 05-04-4915 (to E.S.N.)

Use of ASYM® Plates to repair diaphyseal femoral fractures in two cats

Two intact domestic shorthair cats were presented at our clinic because of grade four signs of lameness at right forelimb. The cats have been hit by car and radiographs revealed a slightly displaced long oblique diaphyseal femoral fractures .The patients tolerated the implant and had a very good functional recovery. Radiographic follow-up after 60 days revealed sign of osseous union. The plates were not removed. This report describes the surgery and outcome of dyaphiseal femoral fractures in two cats repaired by ASYM® Plates

Phosphorylation controls autoinhibition of cytoplasmic linker protein-170

Author Posting. © American Society for Cell Biology, 2010. This article is posted here by permission of American Society for Cell Biology for personal use, not for redistribution. The definitive version was published in Molecular Biology of the Cell 21 (2010): 2661-2673, doi:10.1091/mbc.E09-12-1036.Cytoplasmic linker protein (CLIP)-170 is a microtubule (MT) plus-end-tracking protein that regulates MT dynamics and links MT plus ends to different intracellular structures. We have shown previously that intramolecular association between the N and C termini results in autoinhibition of CLIP-170, thus altering its binding to MTs and the dynactin subunit p150Glued (J. Cell Biol. 2004: 166, 1003–1014). In this study, we demonstrate that conformational changes in CLIP-170 are regulated by phosphorylation that enhances the affinity between the N- and C-terminal domains. By using site-directed mutagenesis and phosphoproteomic analysis, we mapped the phosphorylation sites in the third serine-rich region of CLIP-170. A phosphorylation-deficient mutant of CLIP-170 displays an "open" conformation and a higher binding affinity for growing MT ends and p150Glued as compared with nonmutated protein, whereas a phosphomimetic mutant confined to the "folded back" conformation shows decreased MT association and does not interact with p150Glued. We conclude that phosphorylation regulates CLIP-170 conformational changes resulting in its autoinhibition.This work was supported by National Institutes of Health grant GM-25062 (to G.G.B.); Netherlands Organization for Scientific Research grants (to A. A. and N. G.); a Cancer Genomics Centre grant (to J.v.H.); and Presidential Program of Russian Academy of Sciences and RFBP grant 05-04-4915 (to E.S.N.)