Exploring the synthetic potency of the first furanothioglycoligase through original remote activation.

International audienceThioglycosidic bonds are of utmost importance in biomolecules as their incorporation led to more stable glycomimetics with potential drug activities. Until now only chemical methods were available for their incorporation into glycofuranosyl conjugates. Herein, we wish to describe the use of the first furanothioglycoligase for the preparation of a great variety of thioaryl derivatives with moderate to excellent yields. Of great interest, a stable 1-thioimidoyl arabinofuranose, classically used in chemical glycosylation, was able to efficiently act as a donor through an original enzymatic remote activation mechanism. Study of the chemical structure as well as the nucleophilicity of the thiol allowed us to optimize this biocatalyzed process. As a consequence, this mutated enzyme constitutes an original, mild and eco-friendly method of thioligation

Investigation on the proliferation of Gram negative bacterial cells onto sol-gel carriers

International audienceThis work is related to the synthesis and characterization of new hybrid sol-gel materials. Several carriers based on poly (N-acryloylglycine) (NAGly) composed of poly (ethylene glycol) dimethacrylate (PEGDM) were prepared. The effectiveness of biofilms formation of bacterial strains Escherichia coli and Pseudomonas fluorescents on the matrices was investigated by biochemical methods. Two types of hybrid gels were synthesized based on PEGDM, 2-hydroxyethyl acrylate (HEA) and N-acryloxysuccinimide ester (NAS) with incorporation of inorganic precursors. The rheological properties of the gels and formed biofilms were investigated by quartz crystal microbalance (qCM). The experimental results demonstrated that the obtained matrices are appropriate for biofilms formatio