Sorption of microorganisms by wide-porous agarose cryogels containing grafted aliphatic chains of different length

The possibility of fractionation of heterogeneous bacterial populations using wide-porous agarose cryogels containing grafted aliphatic groups with the chain lengths of 4, 7, and 12 carbon atoms was demonstrated for the first time. The maximum sorption of vegetative cells of gram-positive bacteria (60%) was shown for the polymeric carrier with the chain length of 4 carbon atoms, while the hypometabolic cells appearing in the population after prolonged (60-day) cultivation were trapped by wide-porous affinity sorbents with C7- and C12- aliphatic groups much better than vegetative cells. © Pleiades Publishing, Ltd 2009

Sorption of microorganisms by wide-porous agarose cryogels containing grafted aliphatic chains of different length

The possibility of fractionation of heterogeneous bacterial populations using wide-porous agarose cryogels containing grafted aliphatic groups with the chain lengths of 4, 7, and 12 carbon atoms was demonstrated for the first time. The maximum sorption of vegetative cells of gram-positive bacteria (60%) was shown for the polymeric carrier with the chain length of 4 carbon atoms, while the hypometabolic cells appearing in the population after prolonged (60-day) cultivation were trapped by wide-porous affinity sorbents with C7- and C12- aliphatic groups much better than vegetative cells. © Pleiades Publishing, Ltd 2009

Sorption of microorganisms by wide-porous agarose cryogels containing grafted aliphatic chains of different length

The possibility of fractionation of heterogeneous bacterial populations using wide-porous agarose cryogels containing grafted aliphatic groups with the chain lengths of 4, 7, and 12 carbon atoms was demonstrated for the first time. The maximum sorption of vegetative cells of gram-positive bacteria (60%) was shown for the polymeric carrier with the chain length of 4 carbon atoms, while the hypometabolic cells appearing in the population after prolonged (60-day) cultivation were trapped by wide-porous affinity sorbents with C7- and C12- aliphatic groups much better than vegetative cells. © Pleiades Publishing, Ltd 2009

Sorption of microorganisms by wide-porous agarose cryogels containing grafted aliphatic chains of different length

The possibility of fractionation of heterogeneous bacterial populations using wide-porous agarose cryogels containing grafted aliphatic groups with the chain lengths of 4, 7, and 12 carbon atoms was demonstrated for the first time. The maximum sorption of vegetative cells of gram-positive bacteria (60%) was shown for the polymeric carrier with the chain length of 4 carbon atoms, while the hypometabolic cells appearing in the population after prolonged (60-day) cultivation were trapped by wide-porous affinity sorbents with C7- and C12- aliphatic groups much better than vegetative cells. © Pleiades Publishing, Ltd 2009

Functional activity of insulinoma cells (INS-1E) and pancreatic islets cultured in agarose cryogel sponges

Here, we describe the preparation, structure, and properties of cryogel sponges, which represent a new type of macroporous biomaterial for tissue engineering. Cryogels were produced through freeze-thawing techniques, either from agarose alone or from agarose with grafted gelatin. The aim of this study was to evaluate agarose cryogel sponges as scaffolds for Culturing both isolated pancreatic islets and insulinoma cells (INS-IE). In order to evaluate the effect of cell entrapment in artificial scaffolds, cell function reflected by insulin secretion and content was studied in cells cultivated for a 2-week period either in Culture plastic plates or in cryogel sponge disks. Our results show that tumor-derived INS-1E cells grown either on plastic or on cryogels do not differ in their proliferation, morphology, insulin release, and intracellular insulin content. However, isolated pancreatic islets cultivated on cryogels sponge show 15-fold higher basal insulin secretion at 3.0 mM glucose than islets cultivated on plastic plates and fail to respond to stimulation with 16.7 mM glucose. In addition, these islets have about 2-fold lower insulin content compared to those grown in plastic plates. It is possible that the cell dysfunction noted in these in vitro experiments is due to the effect of the limited oxygen supply to the islets cultivated in cryogel sponge. Further in vivo Studies are needed to clarify the nature of such an observation since according to previous reports, agarose and gelatin induce new vessel formation supporting enhanced oxygen supply. (c) 2005 Wiley Periodicals, Inc