The three bio-markers expression by immunohistochemistry.

<p>(A) High levels of COX-2 expression in hypopharyngeal squamous cell carcinoma: diffuse cytoplasmic staining. (B) Low levels of COX-2 expression in epithelium adjacent to carcinoma. (C) High levels of CD44v6 expression in hypopharyngeal squamous cell carcinoma: cell membrane staining. (D) High levels of CD44v6 expression in epithelium adjacent to carcinoma as well as staining in carcinoma tissues. (E) High levels of CD147 expression in hypopharyngeal squamous cell carcinoma: diffuse cytoplasmic staining. (F) Low level of CD147 expression in epithelium adjacent to carcinoma.</p

The three bio-markers expression in tumor tissues with and without lymph node metastasis.

<p>(P) High levels of COX-2 expression in tumor tissue with lymph node metastasis. (Q) Low levels of COX-2 expression in tumor tissue without lymph node metastasis. (R) High levels of CD44v6 expression in tumor tissue with lymph node metastasis. (S) Low levels of CD44v6 expression in tumor tissue without lymph node metastasis. (T) High levels of CD147 expression in tumor tissue with lymph node metastasis. (U) Low levels of CD147 expression in tumor tissue without lymph node metastasis.</p

The three bio-markers expression in well, moderate and pooly differentiated tumor tissues.

<p>(G) COX-2 expression in well differentiated tumor tissues. (H) COX-2 expression in moderate differentiated tumor tissues. (I) COX-2 expression in poorly differentiated tumor tissues. (J) CD44v6 expression in well differentiated tumor tissues. (K) CD44v6 expression in moderate differentiated tumor tissues. (L) CD44v6 expression in poorly differentiated tumor tissues. (M) CD147 expression in well differentiated tumor tissues. (N) CD147 expression in moderate differentiated tumor tissues. (O) CD147 expression in poorly differentiated tumor tissues.</p

The three bio-markers expression in carcinoma and adjacent epithelium.

*<p><i>P</i><0.001.</p

Correlation of clinicopathologic characteristics of the patients with immunohistochemistry results.

*<p><i>P</i><0.001.</p

Kaplan-Meier curve for the overall survival of the patients with hypopharyngeal carcinoma, according to the expression of P₅₀ of COX-2.

<p>Kaplan-Meier curve for the overall survival of the patients with hypopharyngeal carcinoma, according to the expression of P₅₀ of COX-2.</p

Kaplan-Meier survival analysis by level of the three bio-markers expression.

*<p>log rank <i>P</i><0.05.</p><p>n₁ is the number of total patients of relative percentile;</p><p>n (%) is the number of dead patients of relative percentile (n/n₁*100%).</p

Effects of OVX, HFD and HFDD on histological change in tibia tissue.

<p>A. Fluorescence staining in tibia tissue. (×40); B. Masson-Goldner trichrome staining in tibia tissue (×40); C. undyed cortical bone change in tibia tissue (×40).</p

Disruption of the IFN-γ gene accelerates hepatic fibrosis and partially abolish poly I∶C-mediated suppression.

<p>(A–C) Wild-type and IFN-γ−/− mice were infected with 18–20 cercariae of <i>S. japonicum</i>, and injected with poly I∶C (0.5 µg/g, i.p.) or saline from week 5 to week 10 post-infection. All mice were euthanized at week 10 post-infection. (A–B) Liver tissues were subjected to stain for quantifying collage (A) and α-SMA positive areas (B). Data were shown as mean ± SEM from 7–9 mice per each group. *, <i>P</i><0.05 versus corresponding saline-treated wild-type mice. #, <i>P</i><0.05 versus corresponding poly I∶C-treated wild-type mice. (C) Intrahepatic IL-4, IL-5, and IL-13 mRNA expression levels were measured by quantitative PCR. Results were expressed as fold amplification over normal, uninfected liver following normalization with β-actin. Data were expressed as mean ± SEM (n≥7 for each group).</p

Effects of high-fat diets on the BMD and BMC of right femurs and lumbar vertebras in 13-month-old rats ().

<p>Effects of high-fat diets on the BMD and BMC of right femurs and lumbar vertebras in 13-month-old rats ().</p