Blood Glucose Control Using a Novel Continuous Blood Glucose Monitor and Repetitive Intravenous Insulin Boluses: Exploiting Natural Insulin Pulsatility as a Principle for a Future Artificial Pancreas

The aim of this study was to construct a glucose regulatory algorithm by employing the natural pulsatile pattern of insulin secretion and the oscillatory pattern of resting blood glucose levels and further to regulate the blood glucose level in diabetic pigs by this method. We developed a control algorithm based on repetitive intravenous bolus injections of insulin and combined this with an intravascular blood glucose monitor. Four anesthetized pigs were used in the study. The animals developed a mildly diabetic state from streptozotocin pretreatment. They were steadily brought within the blood glucose target range of 4.5–6.0 mmol/L in 21 to 121 min and kept within that range for 128 to 238 min (hypoglycemic values varied from 2.9 to 51.1 min). The study confirmed our hypotheses regarding the feasibility of this new principle for blood glucose control, and the algorithm was constantly improved during the study to produce the best results in the last animals. The main obstacles were the drift of the IvS-1 sensor and problems with the calibration procedure, which calls for an improvement in the sensor stability before this method can be applied fully in new studies in animals and humans

A Review of the Role of Critical Parameters in the Design and Operation of Biogas Production Plants

Many operating parameters, individually or together, may influence the performance of anaerobic digestion towards biogas or digestate yield and quality maximization. The most preferred method of optimizing an anaerobic digestion plant often relies on how carefully the crucial parameters, such as pH, temperature, organic loading rate, hydraulic retention time, and pressure, are chosen. There is a large amount of literature available on optimization of anaerobic digestion; however, given the continued development and implementation of innovative technologies, together with the introduction of increasingly complex systems, it is necessary to update present knowledge on process parameters and their role on operational ranges and flexibilities in real-life anaerobic digestion system. Accordingly, the present review discusses the importance of the selection of operational parameters in existing technologies and their impact on biogas yield. Notably, the four broad areas of feedstock utilization (substrate, inoculum, codigestion and pretreatment), process condition (pH, temperature, pressure, and reactor design), reactor control (HRT and OLR) and inhibition (Ammonia and VFAs) are covered in this review. In addition, particular emphasis is placed on the most recent innovations that have been or may be implemented in current or future biogas plants