REZALIM-ICIA's method for determination of PAHs from water samples

Polycyclic aromatic hydrocarbons (PAHs) are a class of pollutant chemical compounds consisting of fused aromatic rings with great impact on human health and environment. A modem method for determining these pollutants from water at nanoscale level was developed in REZALIM-ICIA laboratory using an HPLC-FLD instrument. The column, its temperature, the mobile phase and the excitation and emission wavelengths were optimized in order to achieve the proposed goal. Calibration curves for each compound were obtained in 5-60 ng/ml concentration range. The method was tested on spiked samples and the results proved to be satisfying

Fast determination of thiacloprid by photoinduced chemiluminescence

This paper was published in Applied Spectroscopy and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/as/abstract.cfm?URI=as-68-6-642. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.A new and sensitive application of the chemiluminescence detection has been developed for the determination of the pesticide thiacloprid in water. It was based on the on-line photoreaction of thiacloprid in basic medium, with quinine acting as sensitizer of the chemiluminescent response; Cerium (IV) in sulfuric acid medium was used as oxidant. A high automation and reproducibility was provided by a flow injection analysis (FIA) manifold. The validation of the method was performed in terms of selectivity, linearity, LOD, precision and accuracy. Liquid chromatography with UV detection was used as reference for mineral, tap, ground and spring water samples. The proposed method is fast (throughput of 130 h-1), sensitive (LOD of 0.8 ng mL-1 without preconcentration steps and 0.08 ng mL-1 with solid phase extraction (SPE)), low-cost and possible to couple with separative methods for the simultaneous determination of other pesticides. The enhanced chemiluminescence intensity was linear with thiacloprid concentration over the (2-80) and (80-800) ng mL-1 ranges. A possible reaction mechanism is also discussed.The authors thank the Ministerio de Educacion y Ciencia of Spain and the Fondo Europeo de Desarrollo Regional for financial support, Project CTM2006-11991.Catalá Icardo, M.; López Paz, JL.; Pérez Plancha, LM. (2014). Fast determination of thiacloprid by photoinduced chemiluminescence. Applied Spectroscopy. 68(6):642-648. https://doi.org/10.1366/13-07330S642648686Tomizawa, M., & Casida, J. E. (2005). 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THE PRESENT OF PAH’S IN MEAT PRODUCTS FOUND ON THE CLUJ-NAPOCA MARKET

This study focuses on the present of 15 PAH’s in meat products found on the market in Cluj-Napoca. The meat products that were analyzed are: 6 summer salami samples, 1 Hungarian salami sample, 2 pork baloney samples, 2 pork sausages samples and 5 bacon samples. PAH’s have been found in all samples in different quantities ranging from 0,0073 to 88,65,25. The samples were analyses using an HPLC with FLD detection

AN EASY AND RELIABLE METHOD FOR PAH EXTRACTION FROM FOOD SAMPLES

A simple and reliable method for the determination of 16 PAHs from meat and meat products is introduced. The method uses just 10 g of sample and has a high recovery of 70-85%. Prior to the liquid/liquid extraction with cyclohexane the meat samples are saponified using an alcoholic solution of KOH. The samples were purified using a Florosil column and analyzed with a HPLC-FLD instrument. The method was tested on sever meat products that are found on the Cluj-Napoca market with excellent results

EVALUATION OF METALS AND PAH (POLYCYCLIC AROMATIC HYDROCARBONS) CONTAMINATION LEVEL FROM CLUJ-NAPOCA SOIL

Four soil samples (3 from the city exits and one from the Central Park) from Cluj-Napoca were analyzed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and High Performance Liquid Chromatography (HPLC) techniques to establish their level of contamination with metals and polycyclic aromatic hydrocarbons (PAH). The results for metal analysis obtained by ICP-MS showed a contamination of all investigated samples with Ni and As above the normal limits foreseen by Romanian legislation. Also one of the sample presented contamination with Mn above the normal limits. The results for PAH obtained by HPLC show a small contamination with naphthalene, phenanthrene and total PAH in the west exit of Cluj-Napoca. These results suggest that a further investigation of the soil from Cluj-Napoca it is necessary before drawing final conclusions

COMPARATIVE STUDY REGARDING THE TOTAL POLYFENOL CONTENT OF SOME TINCTURES AND OILS FROM ROMANIAN MARKET

In the last decade, there has been much interest in the potential health benefits of plant tinctures because of their high content of polyphenols. 27 samples of tinctures and oils from different plants were analyzed using Folin-Ciocalteu method in order to determine the total polyphenols content. Measurements were compared with the calibration curve of Gallic acid (25, 50, 100, 250, 500 ppm) and the results were expressed as Gallic acid equivalents. Values obtained ranged within very broad limits 6 - 964 mg/L GAE. The lowest concentration was found in Aloe paradisiacum and Aloe marlothi (6 and 9 mg/L GAE) and the heights concentration in Cinnamomum sp. (964 mg/L GAE). The concentration of total polyphenols found in Cinnamomum sp. was 5.6 times bigger than the average concentration of total polyphenols found for the others tinctures (172 mg/L GAE)