Expression of heme oxygenase-1 mRNA in vitreous-treated retinal pigment epithelial cells

Low passage donor retinal pigment epithelial (RPE) cells were incubated with or without vitreous for up to 24 h. RNA was extracted at each time point and heme oxygenase-1 (HO-1) mRNA was measured by qPCR. Ribosomal protein, large, P0 (RPLP0) was used as an internal standard to correct for small differences in the amount of cDNA used in each reaction. Each point at a particular time indicates a different RPE/vitreous donor pair. The number of donor/vitreous pairs examined at each time point is shown. At 6, 12, and 24 h, REST-XL analysis indicated that HO-1 mRNA was significantly increased (p<p><b>Copyright information:</b></p><p>Taken from "Vitreous induces heme oxygenase-1 expression mediated by transforming growth factor-β and reactive oxygen species generation in human retinal pigment epithelial cells"</p><p></p><p>Molecular Vision 2007;13():66-78.</p><p>Published online 24 Jan 2007</p><p>PMCID:PMC2503184.</p><p></p

Vitreous increases c-fos mRNA levels in retinal pigment epithelial cells

Retinal pigment epithelial (RPE) cells were treated for various times with control medium or vitreous-containing medium, and the levels of mRNA for c-fos were measured using qPCR. Each point at a particular treatment time indicates a different RPE/vitreous donor pair, and the number of donor/vitreous pairs is shown. At 30 min, REST-XL analysis showed that c-fos mRNA was significantly increased (*p<p><b>Copyright information:</b></p><p>Taken from "Vitreous induces heme oxygenase-1 expression mediated by transforming growth factor-β and reactive oxygen species generation in human retinal pigment epithelial cells"</p><p></p><p>Molecular Vision 2007;13():66-78.</p><p>Published online 24 Jan 2007</p><p>PMCID:PMC2503184.</p><p></p

Expression of metallothionein mRNAs in vitreous-treated retinal pigment epithelial cells

Retinal pigment epithelial (RPE) cells were incubated with or without vitreous for up to 48 h. RNA was extracted at each time point and RNAs for metallothionein-1a (MT-1a; quadrangle) and metallothionein-2a (MT-2a; diamond) were measured by qPCR. Ribosomal protein, large, P0 (RPLP0) was used as an internal standard to control for small differences in the amount of cDNA used in each reaction. Each point at a particular treatment time indicates a different RPE/vitreous donor pair. The number of donor/vitreous pairs examined at each time point is shown. At all time points, REST-XL analysis indicated that MT-1 and MT-2a mRNAs were significantly increased (p<p><b>Copyright information:</b></p><p>Taken from "Vitreous induces heme oxygenase-1 expression mediated by transforming growth factor-β and reactive oxygen species generation in human retinal pigment epithelial cells"</p><p></p><p>Molecular Vision 2007;13():66-78.</p><p>Published online 24 Jan 2007</p><p>PMCID:PMC2503184.</p><p></p

N-acetyl cysteine has no effect on the vitreous-mediated change in metallothionein expression

Cells were incubated with vitreous for 12 h with or without 2 mM N-acetyl cysteine (NAC). RNA was extracted and metallothionein-1 (MT-1) and metallothionein-2a (MT-2a) mRNA expression was measured by qPCR. Ratios show the change in mRNA expression compared to control (no change=ratio of 1). Asterisk (*) indicates that REST-XL analysis revealed a change that is significantly different from control (p<p><b>Copyright information:</b></p><p>Taken from "Vitreous induces heme oxygenase-1 expression mediated by transforming growth factor-β and reactive oxygen species generation in human retinal pigment epithelial cells"</p><p></p><p>Molecular Vision 2007;13():66-78.</p><p>Published online 24 Jan 2007</p><p>PMCID:PMC2503184.</p><p></p