Observation of a single-beam gradient force acoustical trap for elastic particles: acoustical tweezers

We demonstrate the trapping of elastic particles by the large gradient force of a single acoustical beam in three dimensions. Acoustical tweezers can push, pull and accurately control both the position and the forces exerted on a unique particle. Forces in excess of 1 micronewton were exerted on polystyrene beads in the submillimeter range. A beam intensity less than 50  W/cm^{2} was required, ensuring damage-free trapping conditions. The large spectrum of frequencies covered by coherent ultrasonic sources provides a wide variety of manipulation possibilities from macroscopic to microscopic length scales. Our observations could open the way to important applications, in particular, in biology and biophysics at the cellular scale and for the design of acoustical machines in microfluidic environments

A volumetric display for visual, tactile and audio presentation using acoustic trapping

Science-fiction movies such as Star Wars portray volumetric systems that not only provide visual but also tactile and audible 3D content. Displays, based on swept volume surfaces, holography, optophoretics, plasmonics, or lenticular lenslets, can create 3D visual content without the need for glasses or additional instrumentation. However, they are slow, have limited persistence of vision (POV) capabilities, and, most critically, rely on operating principles that cannot also produce tactile and auditive content. Here, we present for the first time a Multimodal Acoustic Trap Display (MATD): a mid-air volumetric display that can simultaneously deliver visual, auditory, and tactile content, using acoustophoresis as the single operating principle. Our system acoustically traps a particle and illuminates it with red, green, and blue light to control its colour as it quickly scans through our display volume. Using time multiplexing with a secondary trap, amplitude modulation and phase minimization, the MATD delivers simultaneous auditive and tactile content. The system demonstrates particle speeds of up to 8.75m/s and 3.75m/s in the vertical and horizontal directions respectively, offering particle manipulation capabilities superior to other optical or acoustic approaches demonstrated to date. Beyond enabling simultaneous visual, tactile and auditive content, our approach and techniques offer opportunities for non-contact, high-speed manipulation of matter, with applications in computational fabrication and biomedicine

Standing waves for acoustic levitation

Standing waves are the most popular method to achieve acoustic trapping. Particles with greater acoustic impedance than the propagation medium will be trapped at the pressure nodes of a standing wave. Acoustic trapping can be used to hold particles of various materials and sizes, without the need of a close-loop controlling system. Acoustic levitation is a helpful and versatile tool for biomaterials and chemistry, with applications in spectroscopy and lab-on-a-droplet procedures. In this chapter, multiple methods are presented to simulate the acoustic field generated by one or multiple emitters. From the acoustic field, models such as the Gor'kov potential or the Flux Integral are applied to calculate the force exerted on the levitated particles. The position and angle of the acoustic emitters play a fundamental role, thus we analyse commonly used configurations such as emitter and reflector, two opposed emitters, or arrangements using phased arrays

Application of In Vivo Induced Antigen Technology (IVIAT) to Bacillus anthracis

In vivo induced antigen technology (IVIAT) is an immuno-screening technique that identifies bacterial antigens expressed during infection and not during standard in vitro culturing conditions. We applied IVIAT to Bacillus anthracis and identified PagA, seven members of a N-acetylmuramoyl-L-alanine amidase autolysin family, three P60 family lipoproteins, two transporters, spore cortex lytic protein SleB, a penicillin binding protein, a putative prophage holin, respiratory nitrate reductase NarG, and three proteins of unknown function. Using quantitative real-time PCR comparing RNA isolated from in vitro cultured B. anthracis to RNA isolated from BALB/c mice infected with virulent Ames strain B. anthracis, we confirmed induced expression in vivo for a subset of B. anthracis genes identified by IVIAT, including L-alanine amidases BA3767, BA4073, and amiA (pXO2-42); the bacteriophage holin gene BA4074; and pagA (pXO1-110). The exogenous addition of two purified putative autolysins identified by IVIAT, N-acetylmuramoyl-L-alanine amidases BA0485 and BA2446, to vegetative B. anthracis cell suspensions induced a species-specific change in bacterial morphology and reduction in viable bacterial cells. Many of the proteins identified in our screen are predicted to affect peptidoglycan re-modeling, and our results support significant cell wall structural remodeling activity during B. anthracis infection. Identification of L-alanine amidases with B. anthracis specificity may suggest new potential therapeutic targets

Scattering mean free path in continuous complex media: beyond the Helmholtz equation

We present theoretical calculations of the ensemble-averaged (or effective or coherent) wave field propagating in a heterogeneous medium considered as one realization of a random process. In the literature, it is usually assumed that heterogeneity can be accounted for by a random scalar function of the space coordinates, termed the potential. Physically, this amounts to replacing the constant wave speed in Helmholtz' equation by a space-dependent speed. In the case of acoustic waves, we show that this approach leads to incorrect results for the scattering mean free path, no matter how weak the fluctuations. The detailed calculation of the coherent wave field must take into account both a scalar and an operator part in the random potential. When both terms have identical amplitudes, the correct value for the scattering mean free paths is shown to be more than 4 times smaller (13/3, precisely) in the low-frequency limit, whatever the shape of the correlation function. Based on the diagrammatic approach of multiple scattering, theoretical results are obtained for the self-energy and mean free path within Bourret's and on-shell approximations. They are confirmed by numerical experiments

Nanoparticle-coated microbubbles for combined ultrasound imaging and drug delivery

Biomedical microbubbles stabilized by a coating of magnetic or drug-containing nanoparticles show great potential for theranostics applications. Nanoparticle-coated microbubbles can be made to be stable, to be echogenic, and to release the cargo of drug-containing nanoparticles with an ultrasound trigger. This Article reviews the design principles of nanoparticle-coated microbubbles for ultrasound imaging and drug delivery, with a particular focus on the physical chemistry of nanoparticle-coated interfaces; the formation, stability, and dynamics of nanoparticle-coated bubbles; and the conditions for controlled nanoparticle release in ultrasound. The emerging understanding of the modes of nanoparticle expulsion and of the transport of expelled material by microbubble-induced flow is paving the way toward more efficient nanoparticle-mediated drug delivery. This Article highlights the knowledge gap that still remains to be addressed before we can control these phenomena

Virulence of Staphylococcus aureus Small Colony Variants in the Caenorhabditis elegans Infection Model

Small colony variants (SCVs) of Staphylococcus aureus are slow-growing morphological variants that have been implicated in persistent, relapsing, and antibiotic-resistant infections. The altered phenotype of SCVs in most strains has been attributed to defects in electron transport due to mutations in hemin or menadione biosynthesis. The pathogenic capacity of SCVs compared to phenotypically normal strains is variable depending on the attribute examined, with some studies showing reduced virulence of SCVs and others demonstrating normal or heightened virulence. Recently, the nematode Caenorhabditis elegans has been successfully employed as an alternative host to investigate virulence mechanisms of a variety of bacterial pathogens, including S. aureus. In this study, we show that clinical SCVs as well as hemB- and menD-deficient mutants of S. aureus are greatly reduced in virulence in the C. elegans infection model