PoliTo at MULTI-Fake-DetectiVE: Improving FND-CLIP for Multimodal Italian Fake News Detection

The MULTI-Fake-DetectiVE challenge addresses the automatic detection of Italian fake news in a multimodal setting, where both textual and visual components contribute as potential sources of fake content. This paper describes the PoliTO approach to the tasks of fake news detection and analysis of the modality contributions. Our solution turns out to be the best performer on both tasks. It leverages the established FND-CLIP multimodal architecture and proposes ad hoc extensions including sentiment-based text encoding, image transformation in the frequency domain, and data augmentation via back-translation. Thanks to its effectiveness in combining visual and textual content, our solution contributes to fighting the spread of disinformation in the Italian news flow

Recombinant factor VIIa concentrate versus plasma-derived concentrates for treating acute bleeding episodes in people with haemophilia and inhibitors

In people with haemophilia, therapeutic clotting agents might be recognised as a foreign protein and induce anti-factor VIII antibodies, known as 'inhibitors'. Drugs insensitive to such antibodies, either recombinant or plasma-derived, are called factor VIII 'by-passing' agents and used for treatment of bleeding in people with inhibitors

Food Policy Processes in the City of Rome: A Perspective on Policy Integration and Governance Innovation

In the food policy arena, the topic of governance and how to create a governance system that would deal with cross-cutting issues, including new ways of perceiving the public sphere, the policymaking, and the involvement of the population, has become an important field of study. The research presented in this article focuses on the case study of Rome, comparing different paths that various groups of actors have taken toward the definition of urban food policy processes: the Agrifood Plan, Food Policy for Rome, and Community Gardens Movement. The aim of the research is to understand the state of the art about different paths toward food strategies and policies that are currently active in the Roman territory while investigating the relationship between policy integration and governance innovation structures. Indeed, this paper dives into the governance structure of the three food policy processes, the actors and sectors involved, and the goals and instruments selected to achieve a more sustainable food system for the city. In this context, their characteristics are analyzed according to an innovative conceptual framework, which, by crossing two recognized theoretical systems, on policy integration and governance innovation frameworks, allows to identify the capacity of policy integration and governance innovation. The analysis shows that every process performs a different form of governance, implemented according to the actor and backgrounds that compose the process itself. The study demonstrates that governance innovation and policy integration are strongly linked and that the conception and application of policy integration changes according to the governance vision that a process has

Communication Concept of DeConSim: a Decentralized Control Simulator for Production Systems

Abstract In recent years, the price pressure and demand for personalised products in manufacturing increased continuously. As a reaction, technologies from the Information and Communication Technology (ICT) sector are applied by the industry, this trend is also known as digitalisation or Industry 4.0. Digitalisation might be effectively supported by simulation of production lines and will become crucial for manufacturing companies who want to benefit from the advances in ICT technologies, thus improving efficiency and competitiveness. Many agent-based simulators exist, nevertheless, most of them were developed for biological and social sciences whose use and specifications differ significantly from industrial applications. The commercial ones are expensive in terms of licensing, support and require specific training. We propose a communication concept for the simulator that we are developing called DeConSim, which aims at decentralized systems and is composed of several modules (representing resources, each with its own task and intelligence) and a core. DeConSim is capable of operating in two different modes: simulation mode and realtime mode. The former is used to achieve pure simulation results as fast as possible. The latter operates in real-time enabling DeConSim, in contrast to other existing simulators, to become the operational core of the control communication infrastructure of a real production line

Isolated hepatocytes versus hepatocyte spheroids: in vitro culture of rat hepatocytes.

The use of hepatocytes that express liver-specific functions to develop an artificial liver is promising. Unfortunately, the loss of specialized liver functions (dedifferentiation) is still a major problem. Different techniques, such as collagen entrapment, spherical multicellular aggregates (spheroids), and coculture of hepatocytes with extracellular matrix, have been used to improve the performance of hepatocytes in culture. The aim of this study was to compare two different models of hepatocyte isolation in culture: isolated hepatocytes (G1) and hepatocyte spheroids (60% hepatocytes, 40% nonparenchymal cells, and extracellular matrix) (G2). To test functional activity of hepatocytes, both synthetic and metabolic, production of albumin and benzodiazepine transformation into metabolites was tested. G2 showed a high albumin secretion, while a decrease after 15 days of culture in G1 was noted. Diazepam metabolites were higher in G2 than in G1 in all samples, but had statistical significance at days 14 and 21 (p < 0.01). The glycogen content, after 30 days of culture, was very low in G1 (14.2 ± 4.4%), while in G2 it was 72.1 ± 2.6% (p < 0.01). Our study confirms the effectiveness of a culture technique with extracellular matrix and nonparenchymal cells. Maintenance of a prolonged functional activity has been related to restoration of cell polarity and close cell-to-cell contact. We showed that isolated hepatocytes maintain their functional activity for a period significantly reduced, when compared to the hepatocyte spheroids. We confirmed the role of extracellular matrix as a crucial component to promote hepatocyte homeostasis, and the close link between cellular architecture and tissue-specific functions

The energy sensor AMPK regulates Hedgehog signaling in human cells through a unique Gli1 metabolic checkpoint

Hedgehog signaling controls proliferation of cerebellar granule cell precursors (GCPs) and its aberrant activation is a leading cause of Medulloblastoma, the most frequent pediatric brain tumor. We show here that the energy sensor AMPK inhibits Hh signaling by phosphorylating a single residue of human Gli1 that is not conserved in other species.Studies with selective agonists and genetic deletion have revealed that AMPK activation inhibits canonical Hh signaling in human, but not in mouse cells. Indeed we show that AMPK phosphorylates Gli1 at the unique residue Ser408, which is conserved only in primates but not in other species. Once phosphorylated, Gli1 is targeted for proteasomal degradation. Notably, we show that selective AMPK activation inhibits Gli1-driven proliferation and that this effect is linked to Ser408 phosphorylation, which represents a key metabolic checkpoint for Hh signaling.Collectively, this data unveil a novel mechanism of inhibition of Gli1 function, which is exclusive for human cells and may be exploited for the treatment of Medulloblastoma or other Gli1 driven tumors

miRNAs as serum biomarkers for Duchenne muscular dystrophy

Dystrophin absence in Duchenne muscular dystrophy (DMD) causes severe muscle degeneration. We describe that, as consequence of fibre damage, specific muscle-miRNAs are released in to the bloodstream of DMD patients and their levels correlate with the severity of the disease. The same miRNAs are abundant also in the blood of mdx mice and recover to wild-type levels in animals ‘cured’ through exon skipping. Even though creatine kinase (CK) blood levels have been utilized as diagnostic markers of several neuromuscular diseases, including DMD, we demonstrate that they correlate less well with the disease severity. Although the analysis of a larger number of patients should allow to obtain more refined correlations with the different stages of disease progression, we propose that miR-1, miR-133, and miR-206 are new and valuable biomarkers for the diagnosis of DMD and possibly also for monitoring the outcomes of therapeutic interventions in humans. Despite many different DMD therapeutic approaches are now entering clinical trials, a unifying method for assessing the benefit of different treatments is still lacking

Detecting failure of a material handling system through a cognitive twin

This paper describes a methodology for developing a digital twin (DT) based on a rich semantic model and principles of system engineering. The aim is to provide a general model of digital twins (DT) that can improve decision making based on semantic reasoning on real-time system monitoring. The methodology has been tested on a laboratory pilot plant that acts as a material handling system. The key contribution of this research is to propose a generic information model for DT using foundational ontology and principles of systems engineering. The efficacy of the proposed methodology is demonstrated by the automatic detection of a component level failure using semantic reasoning

Mitogen-activated kinase kinase kinase 1 inhibits hedgehog signaling and medulloblastoma growth through GLI1 phosphorylation

The aberrant activation of hedgehog (HH) signaling is a leading cause of the development of medulloblastoma, a pediatric tumor of the cerebellum. The FDA‑approved HH inhibitor, Vismodegib, which targets the transmembrane transducer SMO, has shown limited efficacy in patients with medulloblastoma, due to compensatory mechanisms that maintain an active HH‑GLI signaling status. Thus, the identification of novel actionable mechanisms, directly affecting the activity of the HH‑regulated GLI transcription factors is an important goal for these malignancies. In this study, using gene expression and reporter assays, combined with biochemical and cellular analyses, we demonstrate that mitogen‑activated kinase kinase kinase&nbsp;1&nbsp;(MEKK1), the most upstream kinase of the mitogen‑activated protein kinase&nbsp;(MAPK) phosphorylation modules, suppresses HH signaling by associating and phosphorylating GLI1, the most potent HH‑regulated transcription factor. Phosphorylation occurred at multiple residues in the C‑terminal region of GLI1 and was followed by an increased association with the cytoplasmic proteins&nbsp;14‑3‑3. Of note, the enforced expression of MEKK1 or the exposure of medulloblastoma cells to the MEKK1 activator, Nocodazole, resulted in a marked inhibitory effect on GLI1 activity and tumor cell proliferation and viability. Taken together, the results of this study shed light on a novel regulatory mechanism of HH signaling, with potentially relevant implications in cancer therapy