2 research outputs found
Cell-penetrating peptide conjugates to enhance the antitumor effect of paclitaxel on drug-resistant lung cancer
<p>To conquer the drug resistance of tumors and the poor solubility of paclitaxel (PTX), two PTX-cell-penetrating peptide conjugates (PTX-CPPs), PTX-TAT and PTX-LMWP, were synthesized and evaluated for the first time. Compared with free PTX, PTX-CPPs displayed significantly enhanced cellular uptake, elevated cell toxicity, increased cell apoptosis, and decreased mitochondrial membrane potential (Δψm) in both A549 and A549T cells. PTX-LMWP exhibited a stronger inhibitory effect than PTX-TAT in A549T cells. Analysis of cell-cycle distribution showed that PTX-LMWP influenced mitosis in drug-resistant A549T tumor cells via a different mechanism than PTX. PTX-CPPs were more efficient in inhibiting tumor growth in tumor-bearing mice than free PTX, which suggested their better <i>in vivo</i> antitumor efficacy. Hence, this study demonstrates that PTX-CPPs, particularly PTX-LMWP, have outstanding potential for inhibiting the growth of tumors and are a promising approach for treating lung cancer, especially drug-resistant lung cancer.</p
Peptide-22 and Cyclic RGD Functionalized Liposomes for Glioma Targeting Drug Delivery Overcoming BBB and BBTB
Chemotherapy
outcomes for the treatment of glioma remain unsatisfied due to the
inefficient drug transport across BBB/BBTB and poor drug accumulation
in the tumor site. Nanocarriers functionalized with different targeting
ligands are considered as one of the most promising alternatives.
However, few studies were reported to compare the targeting efficiency
of the ligands and develop nanoparticles to realize BBB/BBTB crossing
and brain tumor targeting simultaneously. In this study, six peptide-based
ligands (Angiopep-2, T7, Peptide-22, cÂ(RGDfK), D-SP5 and Pep-1), widely
used for brain delivery, were selected to decorate liposomes, respectively,
so as to compare their targeting ability to BBB or BBTB. Based on
the <i>in vitro</i> cellular uptake results on BCECs and
HUVECs, Peptide-22 and cÂ(RGDfK) were picked to construct a BBB/BBTB
dual-crossing, glioma-targeting liposomal drug delivery system cÂ(RGDfK)/Pep-22-DOX-LP. <i>In vitro</i> cellular uptake demonstrated that the synergetic
effect of cÂ(RGDfK) and Peptide-22 could significantly increase the
internalization of liposomes on U87 cells. <i>In vivo</i> imaging further verified that cÂ(RGDfK)/Pep-22-LP exhibited higher
brain tumor distribution than single ligand modified liposomes. The
median survival time of glioma-bearing mice treated with cÂ(RGDfK)/Pep-22-DOX-LP
(39.5 days) was significantly prolonged than those treated with free
doxorubicin or other controls. In conclusion, the cÂ(RGDfK) and Peptide-22
dual-modified liposome was constructed based on the targeting ability
screening of various ligands. The system could effectively overcome
BBB/BBTB barriers, target to tumor cells and inhibit the growth of
glioma, which proved its potential for improving the efficacy of chemotherapeutics
for glioma therapy