1 research outputs found
Highly Sensitive SERS Quantification of the Oncogenic Protein c‑Jun in Cellular Extracts
A surface-enhanced
Raman scattering (SERS)-based sensor was developed
for the detection of the oncoprotein c-Jun at nanomolar levels. c-Jun
is a member of the bZIP (basic zipper) family of dimeric transcriptional
activators, and its overexpression has been associated with carcinogenic
mechanisms in several human cancers. For our sensing purpose, we exploited
the ability of c-Jun to heterodimerize with its native protein partner,
c-Fos, and therefore designed a c-Fos peptide receptor chemically
modified to incorporate a thiophenol (TP) group at the N-terminal
site. The TP functionality anchors the c-Fos protein onto the metal
substrate and works as an effective SERS probe to sense the structural
rearrangements associated with the c-Fos/c-Jun heterodimerization