Additional file 5: Table S5. of mRNA-seq reveals skeletal muscle atrophy in response to handling stress in a marine teleost, the red cusk-eel (Genypterus chilensis)

Primer sequences for qPCR assay, amplicon size and PCR. (XLSX 10 kb

Additional file 1: Table S1. of mRNA-seq reveals skeletal muscle atrophy in response to handling stress in a marine teleost, the red cusk-eel (Genypterus chilensis)

Complete list of up-regulated and down-regulated transcripts in response to handling stress. (XLSX 66 kb

Additional file 2: Table S2. of mRNA-seq reveals skeletal muscle atrophy in response to handling stress in a marine teleost, the red cusk-eel (Genypterus chilensis)

Enriched Molecular functions of up-regulated and down-regulated transcripts in response to handling stress. (XLSX 10 kb

Over-represented functional categories in clusters of up-regulated transcripts in response to handling stress.

<p>Over-represented functional categories in clusters of up-regulated transcripts in response to handling stress.</p

Plasmatic levels of ALT (alanine aminotransferase), AST (aspartate aminotransferasa), and AP (alkaline phosphatase).

<p>All data are represented as means ± SEM (n = 4). Significant differences between control and stressed groups are shown as * (P < 0.05) and ** (P < 0.01).</p

Hepatic metabolites levels of glycogen, triglyceride, cholesterol and non-esterified fatty acids (NEFA).

<p>All data are represented as means ± SEM (n = 4). Significant differences between control and stressed groups are shown as * (P < 0.05) and ** (P < 0.01).</p

Quantitative real time PCR validation of differentially expressed transcripts.

<p>Expression of fold changes measured by RNA-seq and qPCR are indicated in the grey and black columns, respectively. <i>agtr2</i> (type-2 angiotensin ii receptor), <i>anxa2</i> (annexin a2), <i>cav1</i> (caveolin-1), <i>ctgf</i> (connective tissue growth factor precursor), <i>jun</i> (transcription factor ap-1), <i>lepr</i> (leptin receptor precursor), <i>plat</i> (tissue-type plasminogen activator precursor), <i>plxnd1</i> (plexin-b2 precursor), <i>pkd1</i> (polycystin-1 precursor), <i>s1pr1</i> (sphingosine 1-phosphate receptor 1), <i>thbs1</i> (thrombospondin-2 precursor), <i>tgfbr3</i> (transforming growth factor beta receptor type 3 precursor), <i>hmgcr</i> (3-hydroxy-3-methylglutaryl-coenzyme a reductase), <i>hmgcs1</i> (hydroxymethylglutaryl- cytoplasmic), <i>dhcr7</i> (7-dehydrocholesterol reductase), <i>nsdhl</i> (sterol-4-alpha-carboxylate 3- decarboxylating), <i>acat2</i> (acetyl-CoA acetyltransferase), <i>c14orf1</i> (ergosterol biosynthetic protein), <i>cyp51a1</i> (lanosterol 14-alpha demethylase), <i>cyb5r3</i> (nadh-cytochrome b5 reductase 3), <i>fdps</i> (farnesyl pyrophosphate synthase), <i>tecr</i> (trans- enoyl- reductase), <i>hsd17b7</i> (3-keto-steroid reductase), <i>idi1</i> (isopentenyl-diphosphate delta-isomerase 1), <i>lss</i> (lanosterol synthase), <i>mvd</i> (diphosphomevalonate decarboxylase), <i>fau</i> (40S ribosomal protein). Significant differences between the control and stressed groups are shown as * (P < 0.05) and ** (P < 0.01).</p