A) Titration curve of humAb binding to HBsAg in an ELISA.

<p>Diamonds: ADRI-2F3, Squares: PK-3D1, Triangles PK-10C7. B) Western blot showing reactivity of humAb ADRI-2F3 (lanes 1 and 2), PK-10C7 (lane 3) and PK-3D1 (lane 4). Lane 5; positive control serum, lane 6; negative control. MW indicates a molecular weight marker. Note that in lanes 3, 4 and 5 a 24 kDa band is recognized, indicating binding to the HBsAg polypeptide. C) Competitive inhibition of serial dilutions (0 ng/ml—400 ng/ml) of humAb ADRI-2F3, and its corresponding recombinant recADR12F3, binding to HBsAg by 200 ng of murine mAb Hyb-824 specific for the HBsAg common “a” determinant. D) Competitive inhibition of humAb ADRI-2F3, recADRI2F3, PK-10C7, PK-3D1 by murine mAb Hyb-824. CM3B6, a HCV-NS3 specific humAb, served as negative control. Co20-F10 is an isotype control.</p

Neutralizing activity of three human monoclonal antibodies PK-3D1, PK-10C7 and ADRI-2F3 in comparison with mouse monoclonal antibody MA18/7.

<p>It is shown herein that a dilution of 1:10,000 of monoclonal antibody supernatant ADRI-2F3 completely neutralizes the activity of HBV, whereas EC50 is reached at a dilution of 1: 100,000.</p