Construction and characterization of the ex-situ modified macroporous bacterial cellulose scaffold as a potential epidermal graft

Background: Skin is a 3-dimensional (3-D) tissue that mainly consists 2 layers, comprising the epidermis and dermis. Skin tissue engineering scaffolds are used commonly as 3-D analogs of the extracellular matrix (ECM) of the skin. Bacterial cellulose (BC) has great importance in skin tissue engineering because of its resemblance to ECM and its biocompatibility. The lack of 3-D microporosity and limited biodegradation capacity has restricted its application as a scaffold for skin tissue engineering. Controlled 3-D microporosity of BC via surface modification techniques are required for potential tissue engineering applications.Methods: Freeze-drying is an ex-situ surface modification technique for making macroporous BC scaffolds (MBCSs). This study proposed a new approach to the freeze-drying method for the arrangement of the pore size of MBCSs specifically for the human keratinocyte cell line (KER-CT). Different concentrations of MBCS (0.25%, 0.50%, and 0.75%) were prepared and the KER-CT cell viability was detected via 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay.Result: The results of this study indicated that the KER-CT cells were able to proliferate all of the concentrations of MBCS, and the best cell viability value was observed with 0.75% MBCS. The results were supported by FESEM and light microscopic observations.Conclusion: These findings suggested that 0.75% MBCS might be of use in epidermal tissue engineering applications

Bazı gram (+) ve gram (-) bakteri grupları arasında elektrotransformasyon yöntemi ile rekombinant bakteri üretimi olasılığının araştırılması

TEZ2139Tez (Yüksek Lisans) -- Çukurova Üniversitesi, Adana, 1996.Kaynakça (s. 72-79) var.iv, 79 s. ; rnk. res. ; 30 cm.

Lipozom-protoplast elektrofüzyon metodu ile Aspergillus niger kökenli glikoamilaz geninin saccharomyces cerevisiae hücrelerine aktarılması ve ekspresyonu

TEZ3548Tez (Doktora) -- Çukurova Üniversitesi, Adana, 2000.Kaynakça (s. 120-127) var.vii, 128 s. : rnk. res. ; 30 cm.…Bu çalışma Ç.Ü. Bilimsel Araştırma Projeleri Birimi Tarafından Desteklenmiştir. Proje No: FBE. 98D. 29

The isolation and characterization of glucoamylase enzyme of an Aspergillus niger natural isolate

Doğal bir izolat olan Aspergillus niger Z10 suşunun, karbon kaynağı olarak sadece %2 oranında çözünür nişasta içeren sıvı Czapek Dox besi ortamından ekstraselüler glikoamilaz enzimi izole edilmiştir. Enzimin Nişastalı-SDS poliakrilamid jel elektroforezi ile moleküler ağırlığının yaklaşık olarak 100.000 Dalton, optimal pH aralığının 4.5-5.5 ve sıcaklığa olan direncinin ise oldukça yüksek olduğu saptanmıştır.An extracellular glucoamylase enzyme was isolated from Aspergillus niger Z10 natural isolate in a liquid Czapek Dox medium which contained soluble starch (2%) as the sole carbon source. The molecular weight of the enzyme was found to be about 100.000 Daltons through Starch-SDS Polyacrylamide Gel Electrophoresis. The optimal pH range of the enzyme was found to be 4.5-5.5 and it was determined that the thermal stability of this enzyme is quite high

Endüstriyel topraklardan izole edilen bazı bakterilerde ağır metal dirençliliğinin araştırılması

Soil samples were collected from an industrial area in Kazanlı, Mersin, Turkey. Six heavy metals, Cr, Cu, Ni, Co, Cd, and Zn, were tested. ICP-AES analyses were carried out to detect the heavy metal levels of the soil samples. The analysis of test samples revealed high levels of Ni and Cr. A total of 272 Pseudomonas spp. and 161 Bacillus spp. strains were isolated from soils from 4 different sampling locations. The maximum tolerable metal concentrations (MTCs) of Cr, Cu, Ni, Co, Cd, and Zn for each isolate were determined. Resistance to Cr was found in 73.9% of the bacterial strains isolated from soil samples, whereas 26% of the isolates exhibited resistance to Ni, 18.4% to Zn, 11.5% to Cd, 9.2% to Co, and 7.3% to Cu. Observed maximum MTCs were 5 mM for Zn and Cu, 3 mM for Ni, and 2 mM for Cr. Plasmids of sizes 1.8, 2.1, and 28 kb were detected in most Pseudomonas spp. strains with resistance to Cu, Cr, Zn, and Ni.Bu çalışmada, Kazanlı / Mersin – Türkiye, endüstri bölgesinden toprak örnekleri toplanmıştır. Cr, Cu, Ni, Co, Cd, and Zn olmak üzere altı ağır metal test edilmiştir. Toprak örneklerindeki ağır metal seviyesini belirlemek için ICP-AES analizleri yapılmıştır. Analizler test örneklerinde yüksek düzeyde Ni ve Cr bulunduğunu göstermiştir. Dört farklı örnekleme bölgesinden toplam 272 adet Pseudomonas spp. ve 161 Bacillus spp. suşu izole edilmiştir. Her bir izolatın Cr, Cu, Ni, Co, Cd ve Zn için MTC değerleri belirlenmiştir. Toprak örneklerinden izole edilen bakterilerin % 73,9’u Cr’a karşı direnç gösterirken, % 26’sı Ni, % 18,4’ü Zn, % 11,5’i Cd, % 9,2’si Co ve % 7,3’ü Cu’a karşı dirençli bulunmuştur. En yüksek MTC’ler, Zn ve Cu için 5 mM, Ni için 3 mM, Cr için 2 mM bulunmuştur. Cu, Cr, Zn ve Ni’e direnç gösteren Pseudomonas spp. suşlarında 1,8, 2,1 ve 28 kb büyüklüğünde ortak plazmidler saptanmıştır

Isolation Of Alcohol Tolerant, Osmotolerant And Thermotolerant Yeast Strains And Improvement Of Their Alcohol Tolerance By Uv Mutagenesis

In this study, the yeast strains were isolated from grapes by serial dilution technique to determine their alcohol-, sugar- and themnotolerance. 34 wild type yeast strains were isolated and alcohol-, sugar- and thermotolerance of these strains were determined. The maximum alcohol tolerance was found to be 9% (v/v) in yeast strain which is named Y2. Thennotolerance behavior of 6 strains were investigated. The strains were treated with UV light with intervals of 20, 30, 40 and 50 seconds. Selected resistant colonies were investigated for alcohol tolerance. It was found that alcohol tolerance increased from 9% (v/v) to 12% (v/v) on Y2 strain

Electrofusion of Saccharomyces cereviciaea Auxotrophic Mutants of Identical Mating Type by Using a Laboratory-made System

Yeast protoplasts from haploid auxotroph strains DC6 (MATa) and FY73 (MATa) were exposed to an inhomogeneous alternating field (AC 200 V/cm). Due to the dielectrophoretic aggregation two or more cells with close membrane contact were formed between the two electrodes. The effects of different DC pulses were investigated to determine critical fusogenic pulse strength. In the results, the maximum fusant cells were obtained only when 8 kV field strengths were applied. These findings showed that the optimum fusogenic pulse strength for yeast protoplasts is 8 kV/cm under our experimental conditions. After fusion, the DNA contents of both hybrid and individual parental strains were compared. The amount of DNA in the hybrid was found to be about twofold higher than that in the individual parental strain

Some properties of Crude Carboxymethyl Cellulase of Aspergillus niger Z10 Wild Type Strain

A carboxymethyl cellulase enzyme was prepared from a wild type strain of Aspergillus niger Z10. Analyses of the enzyme preparation by SDS-PAGE revealed two protein bands showing cellulolytic activity. The molecular weight of these bands was estimated to be around 83,000 and 50,000. The optimum temperature of the enzyme was observed to be around 40°C. It was found that the enzyme's activity has a broad pH range between 3 to 9 and 41.2% of the original activity was retained after heat treatment at 90°C for 15 min