29 research outputs found

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    Compressed file containing: 1) Forward sequence file 2) Reverse sequence file 3) File with experiment name, sample name and the tags, the forward primer and the reverse primer sequence associated

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    Compressed file containing: 1) Forward sequence file 2) Reverse sequence file 3) File with experiment name, sample name and the tags, the forward primer and the reverse primer sequence associated

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    Compressed file containing: 1) Forward sequence file 2) Reverse sequence file 3) File with experiment name, sample name and the tags, the forward primer and the reverse primer sequence associated

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    Compressed file containing: 1) Forward sequence file 2) Reverse sequence file 3) File with experiment name, sample name and the tags, the forward primer and the reverse primer sequence associated

    Environmental DNA reveals quantitative patterns of fish biodiversity in large rivers despite its downstream transportation

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    International audienceDespite the ecological and societal importance of large rivers, fish sampling remains costly and limited to specific habitats (e.g., river banks). Using an eDNA metabarcoding approach, we regularly sampled 500 km of a large river (Rhône River). Comparisons with long-term electrofishing surveys demonstrated the ability of eDNA metabarcoding to qualitatively and quantitatively reveal fish assemblage structures (relative species abundance) but eDNA integrated a larger space than the classical sampling location. Combination of a literature review and field data showed that eDNA behaves in the water column like fine particulate organic matter. Its detection distance varied from a few km in a small stream to more than 100 km in a large river. To our knowledge, our results are the first demonstration of the capacity of eDNA metabarcoding to describe longitudinal fish assemblage patterns in a large river, and metabarcoding appears to be a reliable, cost-effective method for future monitoring

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    File containing experiment name, sample name and the tags, the forward primer and the reverse primer sequence associated

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    File containing experiment name, sample name and the tags, the forward primer and the reverse primer sequence associated

    Run10

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    Compressed file containing: 1) Forward sequence file 2) Reverse sequence file 3) File with experiment name, sample name and the tags, the forward primer and the reverse primer sequence associated
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