The effects of taurine on aminolevulinic acid dehydratase activity in nonylphenol-induced toxicity

The aim of this study was to investigate the effects of taurine on blood aminolevulinic acid dehydratase (ALAD) activity in nonylphenol-induced rats. Forty rats were divided into 5 groups each containing 8 Wistar-albino male rats: control group (C) by standard rat feed, taurine group (T) by standard rat feed+3% taurine (v/w) in drinking water, nonlyphenol group (NP) by standard rat feed+50 ?g kg-1 diet Nonlyphenol, Nonlyphenol+Taurine group (NPT) by standard rat feed+50 ?g kg-1 diet Nonlyphenol+3% taurine (v/w) in drinking water and alcohol group (A) by standard rat feed +50 ?L kg-1 diet alcohol were fed ad libitum for 30 days during the study. The blood ALAD activity significantly increased in T group compared the other experimental groups. Nonlyphenol treatment significantly decreased the blood ALAD activity as compared to control. Decreased levels of blood ALAD activity in NP group were significantly increased in NPT group. The ALAD activity significantly decreased in A group compared the T groups. The results demonstrate that taurine could provide great advantages against to side effects of nonlyphenol toxication on ALAD activity in rats those exposed to Nonylphenol. © 2011 Academic Journals Inc

Effects of shearing procedures on oxidant-antioxidant status in Chios sheep

The aim of the study was to evaluate the stress response and the oxidant/ antioxidant equilibrium against shearing process in sheep. Blood malon-dialdehyde (MDA) and glutathione (GSH) concentrations, plasma cortisol, glucose and cholesterol concentrations as well as plasma total antioxidant activity (AOA) were measured in 18 female Chios sheep one hour before a traditional shearing procedure and immediately after. Circulating MDA and cortisol concentrations were dramatically increased compared to initial values after shearing whereas GSH concentrations were significantly depressed. The variations of the other biochemical parameters were not significant. These results demonstrate that MDA and GSH are the most powerful markers for evaluating the oxidant/antioxidant status and that shearing was a stressful situation leading to an oxidative stress which can be amplified by strong glucocorticoid secretion

The radioprotective potential of Spinacia oleracea and Aesculuc hippocastanum aganist ionizing radiation with their antioxidant and antimicrobial properties

The present study, was designed to determine the possible protective effects of Spinacia oleracea L. extract (S.E) and Aesculuc hippocastanum L. extract (AE) against oxidative tissue damage induced by radiation as well as examinig their in vitro antibacterial potential. The animals were divided totally 4 groups as Control (C), Radiation (R), S.E Extract + Radiation (SER) and AE Extract + Radiation (AER) group. AER and SER groups were also separated to 2 subgroups as SER 1, SER 15, AER 1 and AER 15. C group was administered by 1 mL 0,9 % saline every other day during 20 days. The R group rats were received 1 mL q 0,9% saline every other day i.p. and other day 0.5 Gy radiation during 20 days (n = 10). The other groups, SER and AER were administered by 50 mg kg -1 i.p. S.E and AE extract every other day and other day 0.5 Gy radiations, respectively for 20 days. At the end of experimental period, the animals sacrificed by anesthetizing at 1 (group-C, R, SER 1 and AER 1) and 15 days (group-SER 15 and AER 15) postirradiation. Malondialdehyde (MDA) and reduced Glutathione (GSH) levels in tissues and in vitro antimicrobial activity in the plant extracts were determined. The results indicate that S.E and AE treatment decreases the tissue oxidative stress in irradiation-induced oxidative tissue damage by maintaining the GSH recycling activity and free radical scavenging potential. Moreover, our results demonstrate that, in animals exposed to irradiation, S.E and AE could provide great advantages against to systemic infection from endogenous and exogenous organisms increased after exposure to ionizing radiation. Consequently, the natural compound found in S. oleracea and A. hippocastanum including antioxidants, antimicrobials and other phytonutrients, substantially could be protect the tissue from radiation damage and its complications. © Medwell Journals, 2008

The antioxidant effect of boron on oxidative stress and DNA damage in diabetic rats

The aim of the study was the evaluation of the potentially antioxidant effect of boron against streptozotocin (STZ)-induced diabetes in rats. Animals were allocated into 5 groups of 6 rats each. The control group was fed standard rat feed and received no added treatment. In the diabetic group, STZ was injected intraperitoneally at a single dose of 50 mg/kg. Boron was given to animals in the other groups at dosages of 5, 10, and 20 mgB/kg for 28 days. STZ induced a significant increase of 8-hydroxy-2?-deoxyguanosine as an oxidative stress marker, total oxidant status, oxidative stress index, and DNA damage, whereas it decreased total antioxidant capacity. Also, M30 as an apoptotic marker in a serum was increased by STZ treatment. In addition, pancreatic ?-cells were examined by immunohistochemical methods, and the degeneration of islet cells was observed in STZ-induced diabetic rats. In contrast, boron in a dose-dependent manner decreased amelioration of oxidant status, DNA, and tissue damage in diabetic rats. In conclusion, boron treatment shows an antioxidant effect in diabetes by decreasing oxidative stress and preserving pancreatic ?-cells' integrity

Protective effects of boron on cyclophosphamide induced lipid peroxidation and genotoxicity in rats

PubMed ID: 24530163The aim of the present study was to evaluate the possible protective effect of boron (B) on cyclophosphamide (CYC) induced oxidative stress in rats. Totally, thirty Wistar albino male rats were fed standard rodent diet and divided into 5 equal groups: physiological saline was given intraperitoneally (i.p.) to the control group (vehicle treated), to the second group only 75mgkg-1 CYC was given i.p. on the 14th d, and boron was administered (5, 10, and 20mgkg-1, i.p.) to the other groups for 14 d and CYC (75mgkg-1, i.p.) on the 14th d. CYC caused increase of malondialdehyde and decrease of glutathione levels, decrease of superoxide dismutase activities in erythrocyte and tissues, decrease of erythrocyte, heart, lung, and brain catalase, and plasma antioxidant activities. Also, CYC treatment caused to DNA damage in mononuclear leukocytes. Moreover, B exhibited protective action against the CYC-induced histopathological changes in tissues. However, treatment of B decreased severity of CYC-induced lipid peroxidation and genotoxicity on tissues. In conclusion, B has ameliorative effects against CYC-induced lipid peroxidation and genotoxicity by enhancing antioxidant defence mechanism in rat. © 2014 Elsevier Ltd