The Neighbor net analysis identifies seven distinct genetic clusters.

<p>Neighbor-Net analysis of the concatenated nucleotide sequences based on uncorrected p-distance matrices. Bootstrap values (1,000 replicates) are shown on the edges (percentages). Seven different genetic clusters were identified (I to VII). The MLEE-based taxa and MLSA-based clusters (I to VII) are color-coded. Sub-networks of clusters I, II, IV, VI and VII are magnified and color-coded. Clusters III and V are not magnified due to the low number of genotypes.</p

Geographical distribution of cluster II, VI and VII genotypes.

<p>The neighbor-Net analyses of clusters II, VI and VII (defined in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002255#pntd-0002255-g001" target="_blank">Figure 1</a>) are represented. The genotypes are color-coded according to their geographical origins (detailed in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002255#pntd.0002255.s009" target="_blank">Table S1</a>).</p

Individual Neighbor net analysis of the seven housekeeping genes.

<p>Neighbor-Net analysis of each of the seven gene sequences based on uncorrected p-distance matrices. The seven genetic clusters are color-coded according to <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002255#pntd-0002255-g001" target="_blank">Figure 1</a>.</p

Mass spectra from isolates belonging to either <i>L. (V) braziliensis, L. (V) guyanensis ((V)</i> stands for <i>Viannia</i> subgenus), <i>L. (L) major, L. (L) infantum</i> ((L) stands for <i>Leishmania</i> subgenus).

<p>The two pairs of peaks discriminating the <i>Viannia</i> subgenus from the <i>Leishmania</i> subgenus are labeled in green and blue, respectively and indicated by vertical dotted lines. The 11120+/−(7) peak that identifies the <i>Viannia</i> subgenus is shown in insert squares at the right side of the figure to improve readability. Peaks differentiating species complexes are labeled with their corresponding molecular weights in colored squares. The software automatically provides the molecular weights for all peaks above signal background (grey labels). Peaks that identify species in each subgenus are shown in Black. A few peaks above background were not labeled on the figure to improve readability, the complete spectra are provided as supplementary <b><a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002841#pntd.0002841.s001" target="_blank">figure S1</a></b>.</p

Origin of the imported leishmaniasis cases in metropolitan France, French Guiana, and French West indies (1998–2020).

Maps were designed with the Articque software (https://www.articque.com).</p

Distribution of <i>Leishmania</i> species according to the place of transmission from 1998 to 2020).

(n = 3393, for 2986 other cases species was missing) Maps were designed with the Articque software (https://www.articque.com).</p

Age of patients according to the leishmaniasis clinical form in metropolitan France (n = 509; 8 missing data); red, mean ± standard deviation.

CL: Cutaneous Leishmaniasis; MCL: Muco-Cutaneous Leishmaniasis; VL: Visceral Leishmaniasis. (TIF)</p

Immune profiles among the patients with visceral leishmaniasis in metropolitan France (n = 385; missing data = 22).

(TIF)</p