Western blot analysis of HIF-1α expression in CAL33 cells under normoxia and hypoxia.

<p>A) HIF-1α expression at different times (0–24 h) after setup of hypoxia; 3 h+3 h: incubation of cells done twice for 3 hours under hypoxia, interrupted by a 10 min phase under normoxic conditions. B) HIF-1α expression at different times (0–180 min) after release of CAL33 from hypoxia and incubation under normoxic conditions; control: normoxic cells; CoCl₂: cells treated with CoCl₂ used as positive control; 160 K, 105 K, 75 K molecular weight markers (RPN800, GE Healthcare). Unspecific staining of protein bands other than HIF-1α was used as sample loading control.</p

Relative risk for cell survival/viability after irradiation with photons/²¹³Bi-anti-EGFR-MAb under normoxia/hypoxia.

<p>Relative risk (RR) (▪) denotes RR-fold higher survival of CAL33 cells irradiated under hypoxia compared to normoxia. RR was calculated according to the Generalized Linear Mixed Model (GLMM). Overall cell survival/viability is significantly (RR-fold) higher under hypoxia, if the 95%- confidence interval (•) doesn't include the value 1 (red line).</p

Clonogenic survival and viability of normoxic and hypoxic cells after irradiation with photons or ²¹³Bi-anti-EGFR-MAb.

<p>Results of single experiments are shown as pale coloured graphs, results of means as bold coloured graphs. A) Number of clones (clonogenic assay) and B) absorbance (WST viability assay) as a function of activity concentration (²¹³Bi-anti-EGFR-MAb) or photon dose, respectively.</p