The quantitative FMT light intensity of the orthotopic tumor.

<p>The quantitative FMT light intensity of the orthotopic tumor.</p

The FMT of the orthotopic tumor model.

<p>The coronal degree view of the orthotopic tumor progression and drug response was monitored by the FMT system.</p

The mouse body weight and tumor volume.

<p>A. The mouse body weight of the subcutaneous tumor model. B. The mouse tumor volume of the subcutaneous tumor model.</p

The 3D segmentation images and CD31 immunohistochemistry of the tumor vessels.

<p>A and B show the 3D segmentation images by CTA The white arrows show the microvessel sprouting, the blood vessel near the tumor was marked in red, and the blood vessel away from the tumor was marked in blue. C and D show CD31 immunohistochemistry of the tumor vessels. The black arrow indicates the microvascular areas.</p

The quantitative BLI light intensity of the subcutaneous tumor.

<p>The quantitative BLI light intensity of the subcutaneous tumor.</p

The BLI of subcutaneous tumor model. Continuous BLI light intensity observation of the subcutaneous tumor model from day 0 to day 12.

<p>The BLI of subcutaneous tumor model. Continuous BLI light intensity observation of the subcutaneous tumor model from day 0 to day 12.</p

The Micro-CT/BLI/FMT system and the process of FMT imaging.

<p>A. The schematic of the multi-modality system. The red dashed line represents the BLI/FMT system; the blue dashed line represents the Micro-CT system. B. The figure of the multi-modality system, which is located in the black lead housing. C. The planar fluorescent imaging of mouse orthotopic tumor model at 0°degree, 90°degree, 180°degree and 270°degree.</p

Verifying the validity of the orthotopic tumor model.

<p>Laparotomy was performed on the mouse with the liver exposed in the field of view A, and then the liver was dissected. BLI was carried out again and the optical signal was distributed in the hepatic lobular margins in B. C. The H&E stain revealed that there were tumor nodes in the liver tissue.</p

The 3D FMT reconstruction of the orthotopic tumor model.

<p>A and B. The FMT reconstruction of the orthotopic tumor model and the partial enlarged view.</p

The SLN resection experiments in rabbits.

<p>When the SLN was dissected, we took a photo as shown in a. fluorescent image. The b. color image was acquired at the same time. c. The overlay image also showed the fluorescent position in the color image. After resection, the SLN was put on medical gauze. It was shining brightly as d. the fluorescent image. Although in the e. color image there was no difference in the light, the f. overlay image showed that it was illuminated. All of the dissections were sent in for pathological examination. All of the tissue sections were judged to be the SLN.</p