Variabilità dei principi attivi in <i>Hypericum perforatum</i> L. vegetante in Sardegna

Vista la grande importanza farmacologica che sta rivestendo l’estratto d’iperico nella cura della depressione (2-3) abbiamo ritenuto interessante indagare la specie spontanea presente in Sardegna. Le analisi sono state effettuate via HPLC

Sintesi di nuovi derivati della beta-ciclodestrina carichi negativamente e loro applicazione quali selettori chirali in elettroforesi capillare

In questa sede vengono proposti gli schemi seguiti per la sintesi di nuovi singoli isomeri di: β-ciclodestrine perfunzionalizzate nelle posizioni C2 e C3 e con carica negativa nella posizione C6; β-ciclodestrine perfunzionalizzate con carica negativa nella posizione C6; β-ciclodestrine monofunzionalizzate con carica negativa nella posizione C2

seasonal variation of essential oil in rosmarinus officinalis leaves in sardinia

Rosmarinus officinalis L. is an aromatic plant belonging to the Lamiaceae family widely distributed in the Mediterranean area. The interest on this species is related to the multiple uses of the plant as a food ingredient, in the pharmaceutical and cosmetic industries. The chemical composition of essential oil (EO) from 5 accessions of R. officinalis L., collected monthly through a full year in Sardinia, has been studied by gas chromatography (GC) and GC-mass spectrometry technique. The EO ranged from 0.29% to 0.89%. The qualitative determinations revealed the presence of 27 compounds belonging to 6 chemical groups (hydrocarbon monoterpene, alcohols, aldehydes, ketones, esters, hydrocarbon sesquiterpene). Overall the GC-flame ionization detector analysis showed the presence of 7 major compounds: α-pinene (26%-28%), camphene (5%-8%), 1,8-cineole (15%-25%), borneol (5%-11%), camphor (3%-12%), verbenone (6%-15%), and bornyl acetate (4%-7%). Chromatographic data were also subjected to a chemometric approach that evidenced discrimination of the samples according to the site of collection

Chemical Composition of Thymus Serrulatus Hochst. Ex Benth. Essential Oils from Ethiopia: A Statistical Approach:

From the essential oil (EO) obtained from the aerial parts of T. serrulatus collected in Ethiopia, fifty-three compounds were identified by GC/MS, accounting for more than 99% of the total volatile fraction. Thymol and carvacrol were the main compounds, ranging from 10.0 ± 0.9 to 43.8 ± 3.8% and 4.5 ± 0.4 to 39.1 ± 3.8%, respectively, of the total. o-Cymene, γ-terpinene, and linalool were the most representative compounds in all the EOs

Chemical Composition and Antimicrobial Activities of Essential Oil of Stachys Glutinosa L. from Sardinia

The oil composition of Stachys glutinosa L. from two different areas of Sardinia was analyzed by GC/MS. The oil from Gallura plants was characterized by the four main constituents: terpinen-4-ol (12.7%), α-terpinyl acetate (10.6%), trans-cadina-1(6),4-diene (8.5%), and α-terpineol (8.4%) whilst α-cedrene (19.2%), α-terpineol (18.5%), terpinen-4-ol (12.6%), and α-terpinyl acetate (8.6%) were the main compounds in the oil from Ulassai plants. The oils showed good bacteriostatic activities against Vibrio cholerae (MIC 0.6%), all the Candida tested (1.25%) and Rodotorula rubra (2.5%). There were also bactericidal activities against Candida glabrata (1.25 %) and Rodotorula rubra (2.5%)

Sintesi di nuovi singoli isomeri di -ciclodestrina neutri e carichi negativamente quali selettori chirali in elettroforesi capillare

In questo studio sono stati sintetizzati diversi derivati delle Ciclodestrine (CD) come singoli isomeri sia neutri che anionicamente derivatizzati utilizzando due diversi approcci sintetici uno di selettiva protezione e l'altra di selettiva attivazione dei gruppi funzionali presenti nella struttura CD

Geographical variation of the chemical composition in essential oils extracted from Sardinian Salvia verbenaca

: Salvia verbenaca is a perennial herbaceous plant well appreciated as medicinal herb which can be found in Mediterranean area, Europe, Africa and Asia. In this study, S. verbenaca flowers and leaves were collected in six different geographical areas of Sardinia (Italy). Information about the variation of the chemical composition of plants grown in different locations were obtained from gas chromatography analysis of the extracted essential oils. Gas chromatography analysis detected 33 compounds, which have been grouped in oxygenated monoterpenes, monoterpene hydrocarbons, sesquiterpenes and other compounds. The chemical composition of each group resulted significantly affected by sampling site in terms of geography and altitude. Concerning the geographical distribution of the detected chemicals, sesquiterpenes were found in considerable amount in three localities, monoterpenes hydrocarbons in two and other compounds were predominant only in one site. Regarding the altitude level, monoterpenes, sesquiterpenes and other compounds were predominant, respectively, at medium and medium-high altitude

Variabilità dell'olio essenziale di <i>Helichrysum italicum</i> (Roth) G. Don

Per lo studio dell'olio essenziale di Helichrysum italicum, sono state prese in considerazione varie stazioni di E. italicum (Roth) G. Don subsp. microphyllum (Willd.) Nyman vegetante allo stato spontaneo sia nel Nord che nel Sud della Sardegna. Le analisi sono state condotte via Gas-massa e ci hanno permesso di identificare 69 costituenti per un totale compreso tra il 92.9% ed il 99.9% dei costituenti totali

Profiling of the Bioactive Compounds in Flowers, Leaves and Roots of Vinca sardoa

Vinca sardoa (Stern) Pignatti (Apocynaceae) is largely distributed in Sardinia where it is considered a typical endemism. Since the plant is used in traditional folk medicine, the aim of the present work was to identify and quantify the polyphenolic metabolites, due to the well-known importance of polyphenolic compounds, as well as to evaluate antioxidant activity in different parts of the plant. The compounds were identified and determined in the methanol extracts of leaves, flowers, and roots by developing different LC-MS/MS methods. The obtained data show that leaves possess the highest amount of polyphenols, in particular quinic acid (3401 mg/100 g), chlorogenic acid (1082 mg/100 g), caffeoylquinic acid isomer 1 (190 mg/100 g), and robinin (633 mg/100 g). Likewise, antioxidant tests showed that leaves possess the main radical scavenging activities in both ABTS (49.19 ± 3.41 μg/mL, 30.88 ± 3.04 μg/mL at time zero and after 50 min, respectively) and DPPH assays (223.97 ± 30.81 μg/mL, 109.52 ± 12.89 μg/mL at time zero and after 30 min, respectively). Taking into account that leaves differed most from flowers and roots in the content of caffeoylquinic acid and chlorogenic acid, of which antioxidant properties are widely recognized, it is reasonable to assume that these two compounds are involved in the differences described. The relationship between the high polyphenolic content and interesting antioxidant activities, justifies its use in ethnobotany and may be suggest a use of this specie, after removal of the alkaloid fraction, in the pharmaceutical, phytotherapy, and cosmetic industries

Rosmarinus officinalis L.: chemical modifications of the essential oil and evaluation of antioxidant and antimicrobial activity.

Rosmarinus officinalis essential oil was separated into its hydrocarbon and oxygenated fractions. The major compounds in the hydrocarbon fraction were α-pinene (44.2%), camphene (24.5%), and limonene (11.7%), while in the oxygenated fraction they were 1,8-cineole (37.6%), camphor (16.5%), and bornyl acetate (21.4%). The hydrocarbon fraction was submitted to a hydroformylation process and the antioxidant activity of the product was screened by the DPPH and β-carotene/linoleic acid tests. The hydroformylated fraction maintained the antioxidant activity of the whole oil. The MIC (minimal inhibitory concentration) and the MBC (minimal bactericidal concentration) of the essential oil, hydrocarbon, oxygenated and hydroformylated fractions were also tested on several microorganisms. Aeromonas sobria and Candida strains were the most susceptible micro-organisms. The hydroformylated fraction exhibited a MBC against Candida strains resistant to the other fractions