Control-oriented implementation and model order reduction of a lithium-ion battery electrochemical model

The use of electrochemical models makes it computationally intractable for online implementation as the model is subject to a complicated mathematical structure including partial-differential equations (PDE). This paper is based on the single particle model with electrolyte dynamics. Methods to solve the PDEs in the governing equations are given. Model order reduction techniques are applied to the electrochemical model to reduce the order from 350 to 14. The models solved by numerical solution, residue grouping method and balanced truncation method are compared with experimental data of a coin cell for validation. The results show that the reduced order model can decrease simulation time 75 times compared with the high order model. And the accuracy of the model is kept with 2.3% root mean square error comparing with the experiment results

Multiple-Locus Variable-Number Tandem-Repeat Analysis of Pathogenic Yersinia enterocolitica in China

The predominant bioserotypes of pathogenic Yersinia enterocolitica in China are 2/O: 9 and 3/O: 3; no pathogenic O: 8 strains have been found to date. Multiple-Locus Variable-Number Tandem-Repeat Analysis (MLVA) based on seven loci was able to distinguish 104 genotypes among 218 pathogenic Y. enterocolitica isolates in China and from abroad, showing a high resolution. The major pathogenic serogroups in China, O: 3 and O: 9, were divided into two clusters based on MLVA genotyping. The different distribution of Y. enterocolitica MLVA genotypes maybe due to the recent dissemination of specific clones of 2/O: 9 and 3/O: 3 strains in China. MLVA was a helpful tool for bacterial pathogen surveillance and investigation of pathogenic Y. enterocolitica outbreaks

Identification of stably expressed QTL for resistance to black shank disease in tobacco (Nicotiana tabacum L.) line Beinhart 1000-1

Cigar line Beinhart 1000-1 has effective durable resistance to black shank (BS) and is considered one of the most resistant sources in tobacco (Nicotiana tabacum L.). To investigate the inheritance and identification of stable quantitative trait loci (QTL) for BS response, F2, BC1F2 individuals and BC1F2:3 lines were produced from a cross between Beinhart 1000-1 and Xiaohuangjin 1025. Two major quantitative trait loci (M-QTL) named qBS7 and qBS17 were repeatedly detected under different conditions. QTL qBS7 was mapped to the region between PT30174 and PT60621 and explained 17.40%–25.60% of the phenotypic variance under different conditions. The other QTL qBS17 in interval PT61564–PT61538 of linkage group 17 was detected in a BC1F2 population in the field and in BC1F2:3 in both the field and at the seedling stage, explaining 6.90% to 11.60% of the phenotypic variance. The results improve our understanding of the inheritance of resistance to BS and provide information that can be used in marker-assisted breeding. Keywords: Phytophthora nicotianae, Quantitative trait locus, Plant disease resistance, Simple sequence repeat

Comprehensive genome sequence analysis of Ralstonia solanacearum gd-2, a phylotype I sequevar 15 strain collected from a tobacco bacterial phytopathogen

IntroductionPlant bacterial wilt is an important worldwide disease caused by Ralstonia solanacearum which is a complex of species.MethodsIn this study, we identified and sequenced the genome of R. solanacearum strain gd-2 isolated from tobacco.ResultsStrain gd-2 was identified as R. solanacearum species complex (RSSC) phylotype I sequevar 15 and exhibited strong pathogenicity to tobacco. The genome size of gd-2 was 5.93 Mb, including the chromosomes (3.83 Mb) and the megaplasmid (2.10 Mb). Gene prediction results showed that 3,434 and 1,640 genes were identified in the chromosomes and plasmids, respectively. Comparative genomic analysis showed that gd-2 exhibited high conservation with ten highly similar strain genomes and the differences between gd-2 and other genomes were mainly located at positions GI12-GI14. 72 type III effectors (T3Es) were identified and RipAZ2 was a T3E specific to gd-2 compared with other eight sequenced strain.DiscussionOur study provides a new basis and evidence for studying the pathogenic mechanism of R. solanacearum

Highly and nonpathogenic <i>Y. enterocolitica</i> strains used in this study.

<p>Highly and nonpathogenic <i>Y. enterocolitica</i> strains used in this study.</p

Table_1_Comprehensive genome sequence analysis of Ralstonia solanacearum gd-2, a phylotype I sequevar 15 strain collected from a tobacco bacterial phytopathogen.XLS

IntroductionPlant bacterial wilt is an important worldwide disease caused by Ralstonia solanacearum which is a complex of species.MethodsIn this study, we identified and sequenced the genome of R. solanacearum strain gd-2 isolated from tobacco.ResultsStrain gd-2 was identified as R. solanacearum species complex (RSSC) phylotype I sequevar 15 and exhibited strong pathogenicity to tobacco. The genome size of gd-2 was 5.93 Mb, including the chromosomes (3.83 Mb) and the megaplasmid (2.10 Mb). Gene prediction results showed that 3,434 and 1,640 genes were identified in the chromosomes and plasmids, respectively. Comparative genomic analysis showed that gd-2 exhibited high conservation with ten highly similar strain genomes and the differences between gd-2 and other genomes were mainly located at positions GI12-GI14. 72 type III effectors (T3Es) were identified and RipAZ2 was a T3E specific to gd-2 compared with other eight sequenced strain.DiscussionOur study provides a new basis and evidence for studying the pathogenic mechanism of R. solanacearum.</p

Cluster analysis of 148 pathogenic <i>Y. enterocolitica</i> in Ningxia province.

<p>Cluster analysis of 148 pathogenic <i>Y. enterocolitica</i> in Ningxia province.</p

PFGE result of the strains isolated from Henan province in 2006.

<p>PFGE result of the strains isolated from Henan province in 2006.</p