Additional file 1: Table S1. of Loss of DIP2C in RKO cells stimulates changes in DNA methylation and epithelial-mesenchymal transition

- Primers for generation and validation of isogenic DIP2C cell lines and DIP2C overexpressing cells. Primers are indicated by their number in the text. Primers were purchased from Sigma Aldrich. Table S2. - Primers for RT-qPCR. Primer pairs for amplification of the transcript indicated by the respective name. Primers were purchased from Sigma Aldrich. Table S8. - Summary of DNA methylation analysis in DIP2C −/− cells. Table S9. - Genes with change in methylation (beta diff ≥│0.3│) at promoter sites and gene expression (log2 fold change ≥│2│) in DIP2C −/− #1-1 compared to RKO. (PDF 199 kb

Additional file 2: Table S3. of Loss of DIP2C in RKO cells stimulates changes in DNA methylation and epithelial-mesenchymal transition

- Genes with ≥4 fold change in expression in DIP2C knockout cells. Table S4. - Gene set overlap results for differentially expressed genes (≥4-fold change up or down) investigated with GSEA MSigDB Hallmarks gene set. Table S5. - Functional annotation chart for differentially expressed genes (>4-fold change up or down) investigated with the DAVID Functional annotation tool using the GO Biological process (GO_BP_FAT) annotation category. Table S6. - Genes with ≥│0.3│ units change in median promoter DNA methylation in DIP2C knockout cells. Table S7. - Genes with ≥│0.3│ units change in median gene body DNA methylation in DIP2C knockout cells. Table S10. - Gene set overlap results for promoter differentially methylated genes (≥│0.3│ change in methylation level at promoter sites in DIP2C−/− #1-1) investigated with GSEA MSigDB Hallmarks gene set. (XLSX 152 kb