Additional file 1 of ASTRAL-III: polynomial time species tree reconstruction from partially resolved gene trees

Supplementary material and appendices. Appendices A, B, and C in addition to Figures S1–S8, and Tables S1–S4 are all provided as one Additional file 1. (PDF 476 kb

Total Synthesis of (±)-Minfiensine via a Formal [3+2] Cycloaddition

(±)-Minfiensine (<b>1</b>) was synthesized in 10 steps in 26% overall yield with the 1,2,3,4-tetrahydro-9a,4a-iminoethanocarbazole core constructed through a [3+2] cycloaddition reaction between indole and an azaoxyallylic cation

Legislative Documents

Also, variously referred to as: Senate bills; Senate documents; Senate legislative documents; legislative documents; and General Court documents

Additional file 10: of Genome-wide analysis of GRAS transcription factor gene family in Gossypium hirsutum L.

Table S5. The expression data of qRT-PCR. (XLS 36 kb

Additional file 3: of Genome-wide analysis of GRAS transcription factor gene family in Gossypium hirsutum L.

Figure S6. Phylogenetic trees and evolutionary profile of GRAS genes in green plants. (A) Phylogenetic tree showing the evolutionary relationship between plants (marked with different colors) GRASs. Tree topology is a consensus from NJ, UPGMA. Bootstrap values from the original trees higher than 50% are shown (NJ/UPGMA). (B) Evolutionary profile of GRAS subfamilyes’ genes in green plants. (TIF 1487 kb

Additional file 4: of Genome-wide analysis of GRAS transcription factor gene family in Gossypium hirsutum L.

Figure S2. Chromosomal locations of GRAS members in G. hirsutum L.. (TIF 2000 kb

Additional file 11: of Genome-wide analysis of GRAS transcription factor gene family in Gossypium hirsutum L.

Table S3. The public available expression profile of GRAS members. (XLS 123 kb

Data_Sheet_1_IL-9 Deficiency Promotes Pulmonary Th17 Response in Murine Model of Pneumocystis Infection.PDF

Introduction<p>Pneumocystis pneumonia (PCP) remains a severe complication with high mortality in immunocompromised patients. It has been well accepted that CD4⁺ T cells play a major role in controlling Pneumocystis infection. Th9 cells were the main source of IL-9 with multifaced roles depending on specific diseases. It is unclear whether IL-9/Th9 contributes to the immune response against PCP. The current study aims to explore the role of IL-9 and the effect of IL-9 on Th17 cells in murine model of PCP.</p>Materials and methods<p>Mice were intratracheally injected with 1 × 10⁶Pneumocystis organisms to establish the murine model of Pneumocystis infection. Pneumocystis burden was detected by TaqMan real-time PCR. Using IL-9-deficient (IL-9^−/−) mice, flow cytometry, real-time PCR and enzyme-linked immunosorbent assay (ELISA) were conducted to investigate the immune function related to Th17 response in defense against Pneumocystis infection.</p>Results<p>Reduced Pneumocystis burden was observed in lungs in IL-9^−/− mice compared with WT mice at 3-week postinfection. IL-9^−/−mice exhibited stronger Th17 immune responses than WT PCP mice through flow cytometer and real-time PCR. ELISA revealed higher levels of IL-17 and IL-23 in bronchoalveolar lavage fluid from IL-9^−/− mice than WT mice. And IL-9 deficiency promoted Th17 differentiation from CD4⁺ naive T cells. IL-17A neutralization increased Pneumocystis burden in IL-9^−/− mice.</p>Conclusion<p>Although similar basic clearance of Pneumocystis organisms was achieved in both WT and IL-9^−/− PCP mice, IL-9 deficiency could lower Pneumocystis organism burden and promote pulmonary Th17 cells response in the early stage of infection.</p

Additional file 6: of Genome-wide analysis of GRAS transcription factor gene family in Gossypium hirsutum L.

Figure S4. Motifs describution of GRAS members in G. hirsutum L.. development stages in G. hirsutum L.. (TIF 6550 kb

Additional file 2: of Genome-wide analysis of GRAS transcription factor gene family in Gossypium hirsutum L.

Figure S1. Phylogenetic tree built with the UPGMA method. (TIF 8356 kb