Identification of Five Putative Yeast RNA Helicase Genes

The RNA helicase gene family encodes a group of eight homologous proteins that share regions of sequence similarity. This group of evolutionarily conserved proteins presumably all utilize ATP (or some other nucleoside triphosphate) as an energy source for unwinding double-stranded RNA. Members of this family have been implicated in a variety of physiological functions in organisms ranging from Escherichia coli to human, such as translation initiation, mitochondrial mRNA splicing, ribosomal assembly, and germinal line cell differentiation. We have applied polymerase chain reaction technology to search for additional members of the RNA helicase family in the yeast Saccharomyces cerevisiae. Using degenerate oligonucleotide primers designed to amplify DNA fragments flanked by the highly conserved motifs V L D E A D and Y I H R I G, we have detected five putative RNA helicase genes. Northern and Southern blot analyses demonstrated that these genes are single copy and expressed in yeast. Several members of the RNA helicase family share sequence identity ranging from 49.2% to 67.2%, suggesting that they are functionally related. The discovery of such a multitude of putative RNA helicase genes in yeast suggests that RNA helicase activities are involved in a variety of fundamentally important biological processes

Medication concepts, records, and lists in electronic medical record systems

Thesis (S.M.)--Harvard-MIT Division of Health Sciences and Technology, 2006.Includes bibliographical references.A well-designed implementation of medication concepts, records, and lists in an electronic medical record (EMR) system allows it to successfully perform many functions vital for the provision of quality health care. A controlled medication terminology provides the foundation for decision support services, such as duplication checking, allergy checking, and drug-drug interaction alerts. Clever modeling of medication records makes it easy to provide a history of any medication the patient is on and to generate the patient's medication list for any arbitrary point in time. Medication lists that distinguish between description and prescription and that are exportable in a standard format can play an essential role in medication reconciliation and contribute to the reduction of medication errors. At present, there is no general agreement on how to best implement medication concepts, records, and lists. The underlying implementation in an EMR often reflects the needs, culture, and history of both the developers and the local users. survey of a sample of medication terminologies (COSTAR Directory, the MDD, NDDF Plus, and RxNorm) and EMR implementations of medication records (OnCall, LMR, and the Benedum EMR) reveals the advantages and disadvantages of each. There is no medication system that would fit perfectly in every single context, but some features should strongly be considered in the development of any new system.(cont.) A survey of a sample of medication terminologies (COSTAR Directory, the MDD, NDDF Plus, and RxNorm) and EMR implementations of medication records (OnCall, LMR, and the Benedum EMR) reveals the advantages and disadvantages of each. There is no medication system that would fit perfectly in every single context, but some features should strongly be considered in the development of any new system.by Jaime Chang.S.M

Monte Carlo modeling of low-energy electron-induced secondary electron emission yields in micro-architected boron nitride surfaces

Surface erosion and secondary electron emission (SEE) have been identified as the most critical life-limiting factors in channel walls of Hall-effect thrusters for space propulsion. Recent wall concepts based on micro-architected surfaces have been proposed to mitigate surface erosion and SEE. The idea behind these designs is to take advantage of very-high surface-to-volume ratios to reduce SEE and ion erosion by internal trapping and redeposition. This has resulted in renewed interest to study electron-electron processes in relevant thruster wall materials. In this work, we present calculations of SEE yields in micro-porous hexagonal BN surfaces using stochastic simulations of electron-material interactions in discretized surface geometries. Our model consists of two complementary parts. First we study SEE as a function of primary electron energy and incidence angle in flat surfaces using Monte Carlo simulations of electron multi-scattering processes. The results are then used to represent the response function of discrete surface elements to individual electron rays generated using a ray-tracing Monte Carlo model. We find that micro-porous surfaces result in SEE yield reductions of over 50% in the energy range experienced in Hall thrusters. This points to the suitability of these micro-architected surface concepts to mitigate SEE-related issues in compact electric propulsion devices

Dynamic Cloud Network Control under Reconfiguration Delay and Cost

Network virtualization and programmability allow operators to deploy a wide range of services over a common physical infrastructure and elastically allocate cloud and network resources according to changing requirements. While the elastic reconfiguration of virtual resources enables dynamically scaling capacity in order to support service demands with minimal operational cost, reconfiguration operations make resources unavailable during a given time period and may incur additional cost. In this paper, we address the dynamic cloud network control problem under non-negligible reconfiguration delay and cost. We show that while the capacity region remains unchanged regardless of the reconfiguration delay/cost values, a reconfiguration-agnostic policy may fail to guarantee throughput-optimality and minimum cost under nonzero reconfiguration delay/cost. We then present an adaptive dynamic cloud network control policy that allows network nodes to make local flow scheduling and resource allocation decisions while controlling the frequency of reconfiguration in order to support any input rate in the capacity region and achieve arbitrarily close to minimum cost for any finite reconfiguration delay/cost values.Comment: 15 pages, 7 figure

The Double-edged Sword: A Mixed Methods Study of the Interplay between Bipolar Disorder and Technology Use

Human behavior is increasingly reflected or acted out through technology. This is of particular salience when it comes to changes in behavior associated with serious mental illnesses including schizophrenia and bipolar disorder. Early detection is crucial for these conditions but presently very challenging to achieve. Potentially, characteristics of these conditions\u27 traits and symptoms, at both idiosyncratic and collective levels, may be detectable through technology use patterns. In bipolar disorder specifically, initial evidence associates changes in mood with changes in technology-mediated communication patterns. However much less is known about how people with bipolar disorder use technology more generally in their lives, how they view their technology use in relation to their illness, and, perhaps most crucially, the causal relationship (if any exists) between their technology use and their disease. To address these uncertainties, we conducted a survey of people with bipolar disorder (N = 84). Our results indicate that technology use varies markedly with changes in mood and that technology use broadly may have potential as an early warning signal of mood episodes. We also find that technology for many of these participants is a double-edged sword: acting as both a culprit that can trigger or exacerbate symptoms as well as a support mechanism for recovery. These findings have implications for the design of both early warning systems and technology-mediated interventions

The Watershed tungsten deposit, NE Queensland, Australia: an example of a Permian metamorphic tungsten upgrade after a Carboniferous magmatic-hydrothermal mineralisation event

Tungsten is considered a strategic metal by various countries, including Australia. Between 1998 and 2016 Australia has been steadily increasing its tungsten production, but it is still far smaller than those of the main producers (e.g., China, Russia). Watershed with its current resources of 49.2 Mt averaging 0.14% WO3 is considered one of the biggest undeveloped tungsten deposits outside of China, and if developed would boost Australia’s tungsten production. We will be presenting the geological, geochemical and structural characteristics of the Watershed deposit, as well as the timing, mineral paragenesis and fluid characteristics of the mineralizing system; with the main goal of improving our understanding of the Watershed tungsten deposit and how to explore for similar deposits in northeast Queensland