2 research outputs found
A rare phytosterol, stigmast-5-en-3<i>β</i>,7<i>α</i>,22<i>α</i>-triol and other secondary metabolites from <i>Leea indica</i> showing enhanced <i>in vitro</i> cell migration and proangiogenic activity
Leea indica (Burm. f.) Merr. (Vitaceae) is used for the treatment of wounds in traditional medicine practiced in Sri Lanka. The current study is carried out to investigate its wound healing potential in terms of in vitro cell migration and proangiogenic activity. The scratch wound assay (SWA) guided fractionation of dichloromethane extract of L. indica led to the isolation of a rare phytosterol, stigmast-5-en-3β,7α,22α-triol (1), betulin (2), lupeol (3), and β-sitosterol (4) all of which showed enhanced cell migration in SWA and significant proangiogenic response in chorioallantoic membrane (CAM) assay. The identities of compounds 1–4 were established by the analysis of NMR spectroscopic data and comparison with those reported. This is the first report of the occurrence of compounds 1 and 2 in L. indica.</p
<i>In-vitro</i> cell migration enhancing and pro-angiogenic active secondary metabolites from <i>Jeffreycia zeylanica</i> (L.) H. Rob., S.C. Keeley & Skvarla (Asteraceae)
Jeffreycia zeylanica (L.) H. Rob., S. C. Keeley & Skvarla is used for the treatment of wounds in indigenous medicine practiced in Sri Lanka. The scratch wound assay (SWA) guided fractionation of hexanes extract of J. zeylanica led to the isolation of ethuliacoumarin (1), stigmasterol (2), β-amyrin (3) and lupeol (4) and a non-resolved triterpene alcohol mixture HF5D1, all of which showed enhanced cell migration. The mixture HF5D1 contained glut-5-en-3β-ol (5) and friedelin-3β-ol (6). The identities of compounds 1–6 were established by the analysis of spectroscopic data and comparison of them with those reported. The compounds 1–4 and the non-resolved triterpene alcohol mixture, HF5D1 also exhibited significant proangiogenic response in chorioallantoic membrane (CAM) assay in addition to the enhanced cell migration. This is the first report of the occurrence of the compounds 1, 2, 4 and 5 in this plant.</p